Clostridium perfringens (strain 13 / Type A)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium perfringens

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2721 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P33555|PPDA_CLOPE Prepilin peptidase-dependent protein A OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=ppdA PE=3 SV=2
MM1 pKa = 7.64KK2 pKa = 10.51KK3 pKa = 10.47FICDD7 pKa = 3.32VCGYY11 pKa = 9.92IYY13 pKa = 10.59DD14 pKa = 4.32PAVGDD19 pKa = 3.82PDD21 pKa = 4.19NGVEE25 pKa = 4.44PGTEE29 pKa = 4.11FKK31 pKa = 10.71DD32 pKa = 4.2IPDD35 pKa = 3.79DD36 pKa = 4.03WVCPLCGVDD45 pKa = 4.26KK46 pKa = 11.11SQFSEE51 pKa = 4.65TEE53 pKa = 3.59

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8XH47|Q8XH47_CLOPE Uncharacterized protein OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=CPE2638 PE=4 SV=1
MM1 pKa = 7.46FMTYY5 pKa = 8.42QPKK8 pKa = 9.74KK9 pKa = 7.63RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.69EE15 pKa = 3.5HH16 pKa = 6.2GFRR19 pKa = 11.84KK20 pKa = 9.78RR21 pKa = 11.84MKK23 pKa = 8.75TKK25 pKa = 10.24SGRR28 pKa = 11.84NVLKK32 pKa = 10.5RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.99RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2721

0

2721

855859

37

2104

314.5

35.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.446 ± 0.055

1.121 ± 0.018

5.532 ± 0.039

8.141 ± 0.062

4.591 ± 0.038

6.66 ± 0.048

1.289 ± 0.017

9.568 ± 0.056

9.302 ± 0.056

9.339 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.52 ± 0.022

6.398 ± 0.046

2.725 ± 0.023

1.969 ± 0.021

3.334 ± 0.028

6.208 ± 0.035

4.613 ± 0.031

6.537 ± 0.039

0.694 ± 0.018

4.012 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski