Proteome-pI 2.0 - Proteome Isoelectric Point Database

Beihai weivirus-like virus 18

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1L3KLB4|A0A1L3KLB4_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 18 OX=1922746 PE=4 SV=1

MM1 pKa = 7.24FGEE4 pKa = 4.65SKK6 pKa = 10.55FGSTFPEE13 pKa = 3.94ICEE16 pKa = 4.09TCHH19 pKa = 7.47RR20 pKa = 11.84DD21 pKa = 3.26EE22 pKa = 5.15LQFLSKK28 pKa = 10.17PRR30 pKa = 11.84SSEE33 pKa = 3.73DD34 pKa = 2.81HH35 pKa = 5.22MTRR38 pKa = 11.84VMTIKK43 pKa = 10.46RR44 pKa = 11.84CCTRR48 pKa = 11.84CNNTCFQWPEE58 pKa = 3.53FLGYY62 pKa = 10.45CDD64 pKa = 3.48VTICLPVSLVADD76 pKa = 3.71STSFAQRR83 pKa = 11.84HH84 pKa = 4.17QASTKK89 pKa = 8.88WGIKK93 pKa = 9.17PSCADD98 pKa = 3.42LQLVNLEE105 pKa = 4.21CAARR109 pKa = 11.84DD110 pKa = 3.58PFRR113 pKa = 11.84RR114 pKa = 11.84RR115 pKa = 11.84AYY117 pKa = 9.57LTVGHH122 pKa = 7.05AAMLDD127 pKa = 3.36EE128 pKa = 5.39LVFHH132 pKa = 7.04GPGLLDD138 pKa = 6.04CILQLCCFRR147 pKa = 11.84NAKK150 pKa = 10.28GSTSWRR156 pKa = 11.84LCNSARR162 pKa = 11.84SNQSEE167 pKa = 4.31PPLDD171 pKa = 4.82RR172 pKa = 11.84IQEE175 pKa = 3.99VDD177 pKa = 4.03EE178 pKa = 5.68GDD180 pKa = 4.39DD181 pKa = 3.9NQGDD185 pKa = 4.32DD186 pKa = 4.06VNPAAAHH193 pKa = 5.37SPSRR197 pKa = 11.84NANHH201 pKa = 5.35QQQNGGRR208 pKa = 11.84TEE210 pKa = 4.26AEE212 pKa = 3.99LALEE216 pKa = 4.15QALEE220 pKa = 3.96ASNVEE225 pKa = 4.24EE226 pKa = 3.76QRR228 pKa = 11.84RR229 pKa = 11.84AYY231 pKa = 10.77VEE233 pKa = 4.09GGVLHH238 pKa = 6.19STTTLAPGQRR248 pKa = 11.84PEE250 pKa = 4.43LVEE253 pKa = 4.02PPGLEE258 pKa = 4.22GGCASGTRR266 pKa = 11.84QARR269 pKa = 11.84ARR271 pKa = 11.84FPQITSQPNFLHH283 pKa = 7.0SNNEE287 pKa = 3.66QNLQMAHH294 pKa = 6.77DD295 pKa = 4.05MRR297 pKa = 11.84NVGVGDD303 pKa = 4.16HH304 pKa = 6.83NPLQSEE310 pKa = 4.47VQTRR314 pKa = 11.84DD315 pKa = 3.17RR316 pKa = 11.84LVEE319 pKa = 3.85LLKK322 pKa = 10.9QKK324 pKa = 10.38VFTPGRR330 pKa = 11.84LRR332 pKa = 11.84KK333 pKa = 10.14AMVGFEE339 pKa = 4.23SARR342 pKa = 11.84VSALPKK348 pKa = 10.18KK349 pKa = 10.43LSEE352 pKa = 4.22EE353 pKa = 3.42EE354 pKa = 3.91RR355 pKa = 11.84MRR357 pKa = 11.84VEE359 pKa = 4.24LEE361 pKa = 3.83AMNAALSDD369 pKa = 4.06DD370 pKa = 3.77GVGFDD375 pKa = 3.77TVIKK379 pKa = 10.49AFVKK383 pKa = 10.84SEE385 pKa = 3.74VTAKK389 pKa = 10.63NKK391 pKa = 8.89PRR393 pKa = 11.84PIANHH398 pKa = 5.46GNVRR402 pKa = 11.84LYY404 pKa = 11.29ALAKK408 pKa = 8.44VAYY411 pKa = 9.07AFEE414 pKa = 4.46HH415 pKa = 6.51VMFEE419 pKa = 4.3TFKK422 pKa = 11.07GGSIKK427 pKa = 10.68GRR429 pKa = 11.84GKK431 pKa = 10.39KK432 pKa = 8.81EE433 pKa = 3.96AIEE436 pKa = 3.96EE437 pKa = 4.3LFSNMSNMKK446 pKa = 9.73NGRR449 pKa = 11.84WVEE452 pKa = 3.72NDD454 pKa = 2.97LTSFEE459 pKa = 4.53FGISEE464 pKa = 4.16PLKK467 pKa = 10.27QIEE470 pKa = 3.93MDD472 pKa = 3.34IFYY475 pKa = 9.97HH476 pKa = 5.46IAKK479 pKa = 9.84IIGVADD485 pKa = 3.98TGALLFEE492 pKa = 4.65RR493 pKa = 11.84VSNDD497 pKa = 3.12RR498 pKa = 11.84DD499 pKa = 3.4KK500 pKa = 11.55CATWKK505 pKa = 10.68LIYY508 pKa = 10.1TDD510 pKa = 3.15EE511 pKa = 4.5TGQKK515 pKa = 8.38RR516 pKa = 11.84TAKK519 pKa = 10.08IKK521 pKa = 10.17IPQTMRR527 pKa = 11.84EE528 pKa = 3.95SGDD531 pKa = 3.37RR532 pKa = 11.84VTSSGNFLQNLIAWFCFLVDD552 pKa = 3.56PDD554 pKa = 4.08YY555 pKa = 11.88VEE557 pKa = 5.48DD558 pKa = 4.34ALEE561 pKa = 3.94SLIKK565 pKa = 10.6HH566 pKa = 6.21RR567 pKa = 11.84GSKK570 pKa = 10.18LFYY573 pKa = 9.38VSRR576 pKa = 11.84RR577 pKa = 11.84DD578 pKa = 3.09RR579 pKa = 11.84STIEE583 pKa = 3.82LRR585 pKa = 11.84GKK587 pKa = 7.62QVRR590 pKa = 11.84KK591 pKa = 10.19KK592 pKa = 10.75YY593 pKa = 9.56LACLAFEE600 pKa = 5.22GDD602 pKa = 3.83DD603 pKa = 3.25TSGRR607 pKa = 11.84IEE609 pKa = 4.05EE610 pKa = 4.41PVWLNGPYY618 pKa = 10.55NPDD621 pKa = 3.14NSEE624 pKa = 4.06TKK626 pKa = 10.46DD627 pKa = 3.46DD628 pKa = 4.27CLVSSFFRR636 pKa = 11.84RR637 pKa = 11.84WGWKK641 pKa = 9.75AKK643 pKa = 10.3LIWKK647 pKa = 8.09PQEE650 pKa = 3.21GDD652 pKa = 3.16AYY654 pKa = 10.87VRR656 pKa = 11.84FVGYY660 pKa = 9.12EE661 pKa = 3.87ALIHH665 pKa = 6.61DD666 pKa = 4.62SKK668 pKa = 10.95IVYY671 pKa = 10.12DD672 pKa = 3.85GAEE675 pKa = 3.66MVMTPEE681 pKa = 4.71ARR683 pKa = 11.84RR684 pKa = 11.84LLNTKK689 pKa = 8.93SWTTTAVTPAEE700 pKa = 4.52LKK702 pKa = 8.98TCIRR706 pKa = 11.84VYY708 pKa = 10.5AASLADD714 pKa = 3.31GFKK717 pKa = 10.42RR718 pKa = 11.84VEE720 pKa = 3.73PMYY723 pKa = 11.2AFLNAVFDD731 pKa = 4.44SNKK734 pKa = 10.52GGVDD738 pKa = 3.09VDD740 pKa = 3.62AQKK743 pKa = 11.11VKK745 pKa = 10.58EE746 pKa = 3.98YY747 pKa = 10.6LLAVEE752 pKa = 5.45GEE754 pKa = 4.32MPEE757 pKa = 3.73HH758 pKa = 6.63GRR760 pKa = 11.84TVNCPIDD767 pKa = 3.57MPPFEE772 pKa = 6.7GGDD775 pKa = 3.22ADD777 pKa = 3.05KK778 pKa = 10.65WKK780 pKa = 10.67RR781 pKa = 11.84LLRR784 pKa = 11.84RR785 pKa = 11.84SAGDD789 pKa = 3.12FDD791 pKa = 3.75EE792 pKa = 6.18RR793 pKa = 11.84EE794 pKa = 4.14WATMCHH800 pKa = 6.57ISTIDD805 pKa = 3.06IHH807 pKa = 8.6GEE809 pKa = 3.64DD810 pKa = 4.16LAISVPAEE818 pKa = 3.59WRR820 pKa = 11.84NN821 pKa = 3.21

Molecular weight: 92.39 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1L3KLB4|A0A1L3KLB4_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 18 OX=1922746 PE=4 SV=1

MM1 pKa = 7.24FGEE4 pKa = 4.65SKK6 pKa = 10.55FGSTFPEE13 pKa = 3.94ICEE16 pKa = 4.09TCHH19 pKa = 7.47RR20 pKa = 11.84DD21 pKa = 3.26EE22 pKa = 5.15LQFLSKK28 pKa = 10.17PRR30 pKa = 11.84SSEE33 pKa = 3.73DD34 pKa = 2.81HH35 pKa = 5.22MTRR38 pKa = 11.84VMTIKK43 pKa = 10.46RR44 pKa = 11.84CCTRR48 pKa = 11.84CNNTCFQWPEE58 pKa = 3.53FLGYY62 pKa = 10.45CDD64 pKa = 3.48VTICLPVSLVADD76 pKa = 3.71STSFAQRR83 pKa = 11.84HH84 pKa = 4.17QASTKK89 pKa = 8.88WGIKK93 pKa = 9.17PSCADD98 pKa = 3.42LQLVNLEE105 pKa = 4.21CAARR109 pKa = 11.84DD110 pKa = 3.58PFRR113 pKa = 11.84RR114 pKa = 11.84RR115 pKa = 11.84AYY117 pKa = 9.57LTVGHH122 pKa = 7.05AAMLDD127 pKa = 3.36EE128 pKa = 5.39LVFHH132 pKa = 7.04GPGLLDD138 pKa = 6.04CILQLCCFRR147 pKa = 11.84NAKK150 pKa = 10.28GSTSWRR156 pKa = 11.84LCNSARR162 pKa = 11.84SNQSEE167 pKa = 4.31PPLDD171 pKa = 4.82RR172 pKa = 11.84IQEE175 pKa = 3.99VDD177 pKa = 4.03EE178 pKa = 5.68GDD180 pKa = 4.39DD181 pKa = 3.9NQGDD185 pKa = 4.32DD186 pKa = 4.06VNPAAAHH193 pKa = 5.37SPSRR197 pKa = 11.84NANHH201 pKa = 5.35QQQNGGRR208 pKa = 11.84TEE210 pKa = 4.26AEE212 pKa = 3.99LALEE216 pKa = 4.15QALEE220 pKa = 3.96ASNVEE225 pKa = 4.24EE226 pKa = 3.76QRR228 pKa = 11.84RR229 pKa = 11.84AYY231 pKa = 10.77VEE233 pKa = 4.09GGVLHH238 pKa = 6.19STTTLAPGQRR248 pKa = 11.84PEE250 pKa = 4.43LVEE253 pKa = 4.02PPGLEE258 pKa = 4.22GGCASGTRR266 pKa = 11.84QARR269 pKa = 11.84ARR271 pKa = 11.84FPQITSQPNFLHH283 pKa = 7.0SNNEE287 pKa = 3.66QNLQMAHH294 pKa = 6.77DD295 pKa = 4.05MRR297 pKa = 11.84NVGVGDD303 pKa = 4.16HH304 pKa = 6.83NPLQSEE310 pKa = 4.47VQTRR314 pKa = 11.84DD315 pKa = 3.17RR316 pKa = 11.84LVEE319 pKa = 3.85LLKK322 pKa = 10.9QKK324 pKa = 10.38VFTPGRR330 pKa = 11.84LRR332 pKa = 11.84KK333 pKa = 10.14AMVGFEE339 pKa = 4.23SARR342 pKa = 11.84VSALPKK348 pKa = 10.18KK349 pKa = 10.43LSEE352 pKa = 4.22EE353 pKa = 3.42EE354 pKa = 3.91RR355 pKa = 11.84MRR357 pKa = 11.84VEE359 pKa = 4.24LEE361 pKa = 3.83AMNAALSDD369 pKa = 4.06DD370 pKa = 3.77GVGFDD375 pKa = 3.77TVIKK379 pKa = 10.49AFVKK383 pKa = 10.84SEE385 pKa = 3.74VTAKK389 pKa = 10.63NKK391 pKa = 8.89PRR393 pKa = 11.84PIANHH398 pKa = 5.46GNVRR402 pKa = 11.84LYY404 pKa = 11.29ALAKK408 pKa = 8.44VAYY411 pKa = 9.07AFEE414 pKa = 4.46HH415 pKa = 6.51VMFEE419 pKa = 4.3TFKK422 pKa = 11.07GGSIKK427 pKa = 10.68GRR429 pKa = 11.84GKK431 pKa = 10.39KK432 pKa = 8.81EE433 pKa = 3.96AIEE436 pKa = 3.96EE437 pKa = 4.3LFSNMSNMKK446 pKa = 9.73NGRR449 pKa = 11.84WVEE452 pKa = 3.72NDD454 pKa = 2.97LTSFEE459 pKa = 4.53FGISEE464 pKa = 4.16PLKK467 pKa = 10.27QIEE470 pKa = 3.93MDD472 pKa = 3.34IFYY475 pKa = 9.97HH476 pKa = 5.46IAKK479 pKa = 9.84IIGVADD485 pKa = 3.98TGALLFEE492 pKa = 4.65RR493 pKa = 11.84VSNDD497 pKa = 3.12RR498 pKa = 11.84DD499 pKa = 3.4KK500 pKa = 11.55CATWKK505 pKa = 10.68LIYY508 pKa = 10.1TDD510 pKa = 3.15EE511 pKa = 4.5TGQKK515 pKa = 8.38RR516 pKa = 11.84TAKK519 pKa = 10.08IKK521 pKa = 10.17IPQTMRR527 pKa = 11.84EE528 pKa = 3.95SGDD531 pKa = 3.37RR532 pKa = 11.84VTSSGNFLQNLIAWFCFLVDD552 pKa = 3.56PDD554 pKa = 4.08YY555 pKa = 11.88VEE557 pKa = 5.48DD558 pKa = 4.34ALEE561 pKa = 3.94SLIKK565 pKa = 10.6HH566 pKa = 6.21RR567 pKa = 11.84GSKK570 pKa = 10.18LFYY573 pKa = 9.38VSRR576 pKa = 11.84RR577 pKa = 11.84DD578 pKa = 3.09RR579 pKa = 11.84STIEE583 pKa = 3.82LRR585 pKa = 11.84GKK587 pKa = 7.62QVRR590 pKa = 11.84KK591 pKa = 10.19KK592 pKa = 10.75YY593 pKa = 9.56LACLAFEE600 pKa = 5.22GDD602 pKa = 3.83DD603 pKa = 3.25TSGRR607 pKa = 11.84IEE609 pKa = 4.05EE610 pKa = 4.41PVWLNGPYY618 pKa = 10.55NPDD621 pKa = 3.14NSEE624 pKa = 4.06TKK626 pKa = 10.46DD627 pKa = 3.46DD628 pKa = 4.27CLVSSFFRR636 pKa = 11.84RR637 pKa = 11.84WGWKK641 pKa = 9.75AKK643 pKa = 10.3LIWKK647 pKa = 8.09PQEE650 pKa = 3.21GDD652 pKa = 3.16AYY654 pKa = 10.87VRR656 pKa = 11.84FVGYY660 pKa = 9.12EE661 pKa = 3.87ALIHH665 pKa = 6.61DD666 pKa = 4.62SKK668 pKa = 10.95IVYY671 pKa = 10.12DD672 pKa = 3.85GAEE675 pKa = 3.66MVMTPEE681 pKa = 4.71ARR683 pKa = 11.84RR684 pKa = 11.84LLNTKK689 pKa = 8.93SWTTTAVTPAEE700 pKa = 4.52LKK702 pKa = 8.98TCIRR706 pKa = 11.84VYY708 pKa = 10.5AASLADD714 pKa = 3.31GFKK717 pKa = 10.42RR718 pKa = 11.84VEE720 pKa = 3.73PMYY723 pKa = 11.2AFLNAVFDD731 pKa = 4.44SNKK734 pKa = 10.52GGVDD738 pKa = 3.09VDD740 pKa = 3.62AQKK743 pKa = 11.11VKK745 pKa = 10.58EE746 pKa = 3.98YY747 pKa = 10.6LLAVEE752 pKa = 5.45GEE754 pKa = 4.32MPEE757 pKa = 3.73HH758 pKa = 6.63GRR760 pKa = 11.84TVNCPIDD767 pKa = 3.57MPPFEE772 pKa = 6.7GGDD775 pKa = 3.22ADD777 pKa = 3.05KK778 pKa = 10.65WKK780 pKa = 10.67RR781 pKa = 11.84LLRR784 pKa = 11.84RR785 pKa = 11.84SAGDD789 pKa = 3.12FDD791 pKa = 3.75EE792 pKa = 6.18RR793 pKa = 11.84EE794 pKa = 4.14WATMCHH800 pKa = 6.57ISTIDD805 pKa = 3.06IHH807 pKa = 8.6GEE809 pKa = 3.64DD810 pKa = 4.16LAISVPAEE818 pKa = 3.59WRR820 pKa = 11.84NN821 pKa = 3.21

Molecular weight: 92.39 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	821	821	821	821.0	92.39

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
8.161 ± 0.0	2.68 ± 0.0	6.09 ± 0.0	7.917 ± 0.0	4.507 ± 0.0	6.699 ± 0.0	2.314 ± 0.0	4.019 ± 0.0	5.603 ± 0.0	8.039 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.436 ± 0.0	4.507 ± 0.0	4.507 ± 0.0	3.654 ± 0.0	7.186 ± 0.0	6.334 ± 0.0	5.238 ± 0.0	6.334 ± 0.0	1.705 ± 0.0	2.071 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski