Chitinophaga costaii
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4204 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C4ELG9|A0A1C4ELG9_9BACT HupE / UreJ protein OS=Chitinophaga costaii OX=1335309 GN=GA0116948_10912 PE=4 SV=1
MM1 pKa = 7.06 TALEE5 pKa = 4.4 VSTGAYY11 pKa = 7.48 YY12 pKa = 10.54 TDD14 pKa = 4.55 IIDD17 pKa = 5.06 KK18 pKa = 9.93 EE19 pKa = 4.31 DD20 pKa = 3.23 HH21 pKa = 6.71 RR22 pKa = 11.84 LWQIQQIEE30 pKa = 4.28 NGNSVFEE37 pKa = 4.25 GTAYY41 pKa = 10.46 SQWNGIEE48 pKa = 3.67 WDD50 pKa = 3.43
Molecular weight: 5.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.816
IPC2_protein 4.05
IPC_protein 3.859
Toseland 3.694
ProMoST 3.897
Dawson 3.846
Bjellqvist 4.113
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.617
Solomon 3.808
Lehninger 3.77
Nozaki 3.986
DTASelect 4.113
Thurlkill 3.757
EMBOSS 3.795
Sillero 3.986
Patrickios 1.901
IPC_peptide 3.821
IPC2_peptide 3.961
IPC2.peptide.svr19 3.959
Protein with the highest isoelectric point:
>tr|A0A1C4F0A2|A0A1C4F0A2_9BACT AraC-type DNA-binding protein OS=Chitinophaga costaii OX=1335309 GN=GA0116948_110170 PE=4 SV=1
MM1 pKa = 7.55 KK2 pKa = 10.49 SILLVVLLAFTGAFAASAQTRR23 pKa = 11.84 VVIRR27 pKa = 11.84 PVPVAVRR34 pKa = 11.84 PAVVVAPAVPVVVATPVSPVIVRR57 pKa = 11.84 PAAVVVRR64 pKa = 11.84 PAIRR68 pKa = 11.84 RR69 pKa = 11.84 RR70 pKa = 11.84 VVVVRR75 pKa = 11.84 RR76 pKa = 3.66
Molecular weight: 7.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.515
IPC2_protein 11.184
IPC_protein 12.793
Toseland 12.954
ProMoST 13.466
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.501
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.237
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.194
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4204
0
4204
1471284
26
4319
350.0
39.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.581 ± 0.036
0.824 ± 0.014
5.199 ± 0.027
5.0 ± 0.049
4.563 ± 0.025
6.946 ± 0.033
2.317 ± 0.021
6.403 ± 0.032
5.688 ± 0.034
9.952 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.34 ± 0.02
4.994 ± 0.037
4.297 ± 0.023
4.482 ± 0.026
4.363 ± 0.027
5.941 ± 0.03
6.114 ± 0.056
6.569 ± 0.031
1.251 ± 0.014
4.178 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here