Ashy storm petrel gyrovirus
Average proteome isoelectric point is 8.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z4N430|A0A2Z4N430_9VIRU CA1 OS=Ashy storm petrel gyrovirus OX=2249930 PE=3 SV=1
MM1 pKa = 7.31 SFSVLCRR8 pKa = 11.84 DD9 pKa = 3.86 TLRR12 pKa = 11.84 GTLQYY17 pKa = 10.51 NPKK20 pKa = 9.1 YY21 pKa = 10.53 QRR23 pKa = 11.84 DD24 pKa = 3.41 LANWFRR30 pKa = 11.84 DD31 pKa = 3.42 CSRR34 pKa = 11.84 THH36 pKa = 7.06 RR37 pKa = 11.84 AICRR41 pKa = 11.84 CPSYY45 pKa = 10.62 RR46 pKa = 11.84 NHH48 pKa = 6.55 FKK50 pKa = 10.81 APKK53 pKa = 8.97 PQTQEE58 pKa = 4.0 ASTQTEE64 pKa = 4.23 RR65 pKa = 11.84 PPKK68 pKa = 10.36 SVTILPARR76 pKa = 11.84 RR77 pKa = 11.84 GILSSPKK84 pKa = 9.86 DD85 pKa = 3.52 RR86 pKa = 11.84 SPGKK90 pKa = 9.91 RR91 pKa = 11.84 NKK93 pKa = 9.52 ILHH96 pKa = 6.75 FDD98 pKa = 3.68 PFLNEE103 pKa = 3.85 ALYY106 pKa = 10.35 HH107 pKa = 5.3 SKK109 pKa = 10.38 RR110 pKa = 11.84 RR111 pKa = 11.84 RR112 pKa = 11.84 KK113 pKa = 9.39 QLLQEE118 pKa = 5.17 RR119 pKa = 11.84 PDD121 pKa = 3.16 NWHH124 pKa = 6.93 PLWEE128 pKa = 4.14 ITPPLKK134 pKa = 10.78 ACLDD138 pKa = 3.79 FDD140 pKa = 4.19 DD141 pKa = 5.02 TEE143 pKa = 4.59 EE144 pKa = 4.55 DD145 pKa = 3.82 TDD147 pKa = 4.1 TSDD150 pKa = 4.34 DD151 pKa = 3.95 DD152 pKa = 4.58 GGAGEE157 pKa = 5.13 TDD159 pKa = 4.27 DD160 pKa = 5.6 EE161 pKa = 6.15 DD162 pKa = 3.87 DD163 pKa = 4.14 TVPHH167 pKa = 6.46 VDD169 pKa = 4.25 FDD171 pKa = 3.57 IGINGIVDD179 pKa = 5.14 GIPPEE184 pKa = 4.38 PSDD187 pKa = 3.63 ASSSNRR193 pKa = 11.84 TLEE196 pKa = 3.92 QNPYY200 pKa = 10.25 GLPNHH205 pKa = 5.74 TTHH208 pKa = 7.15 SEE210 pKa = 4.05 LLSKK214 pKa = 10.82 VLFSSHH220 pKa = 5.35 KK221 pKa = 9.24 QQ222 pKa = 3.17
Molecular weight: 25.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.904
IPC2_protein 5.779
IPC_protein 5.842
Toseland 6.262
ProMoST 6.198
Dawson 6.148
Bjellqvist 6.135
Wikipedia 6.173
Rodwell 6.135
Grimsley 6.415
Solomon 6.148
Lehninger 6.148
Nozaki 6.415
DTASelect 6.62
Thurlkill 6.634
EMBOSS 6.605
Sillero 6.517
Patrickios 4.38
IPC_peptide 6.16
IPC2_peptide 6.517
IPC2.peptide.svr19 6.457
Protein with the highest isoelectric point:
>tr|A0A2Z4N434|A0A2Z4N434_9VIRU ORF3 OS=Ashy storm petrel gyrovirus OX=2249930 PE=4 SV=1
MM1 pKa = 7.41 SRR3 pKa = 11.84 FRR5 pKa = 11.84 RR6 pKa = 11.84 YY7 pKa = 9.48 RR8 pKa = 11.84 GRR10 pKa = 11.84 YY11 pKa = 7.71 GYY13 pKa = 10.45 LGRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 WRR20 pKa = 11.84 WRR22 pKa = 11.84 NRR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 YY29 pKa = 9.43 RR30 pKa = 11.84 PTRR33 pKa = 11.84 RR34 pKa = 11.84 LRR36 pKa = 11.84 YY37 pKa = 8.22 RR38 pKa = 11.84 YY39 pKa = 9.59 KK40 pKa = 10.2 RR41 pKa = 11.84 YY42 pKa = 9.79 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 NSARR49 pKa = 11.84 AIRR52 pKa = 11.84 RR53 pKa = 11.84 LFFKK57 pKa = 10.03 PNPGTEE63 pKa = 4.14 PVRR66 pKa = 11.84 ITKK69 pKa = 9.79 PYY71 pKa = 9.66 NSLGVTFQGIILIPQTVAAWSLNVPQDD98 pKa = 3.16 QTDD101 pKa = 3.37 NYY103 pKa = 9.97 ISSRR107 pKa = 11.84 VAKK110 pKa = 10.09 IDD112 pKa = 3.37 VSFANLLRR120 pKa = 11.84 AVLPLDD126 pKa = 4.27 YY127 pKa = 9.85 ISKK130 pKa = 10.18 IGGPIPGRR138 pKa = 11.84 LQSQVTGQPSDD149 pKa = 3.6 TPPPKK154 pKa = 10.24 TGWPYY159 pKa = 11.17 AKK161 pKa = 10.38 PMTQDD166 pKa = 2.92 GQTPTSNPSEE176 pKa = 3.83 WWRR179 pKa = 11.84 WALLLMHH186 pKa = 6.9 PTDD189 pKa = 3.3 NVRR192 pKa = 11.84 FLTTPQIMNTEE203 pKa = 3.84 EE204 pKa = 3.94 MGAMFAQYY212 pKa = 10.61 QLLRR216 pKa = 11.84 HH217 pKa = 5.94 EE218 pKa = 4.45 FTRR221 pKa = 11.84 FKK223 pKa = 11.11 VEE225 pKa = 4.59 CIDD228 pKa = 4.95 DD229 pKa = 5.02 GGLKK233 pKa = 9.58 WSPVASLAIQNQYY246 pKa = 9.83 WEE248 pKa = 4.62 IKK250 pKa = 10.25 SNPTSPYY257 pKa = 7.29 TAHH260 pKa = 7.01 TIVQPGTQTEE270 pKa = 4.4 IVNQATGPRR279 pKa = 11.84 TPTDD283 pKa = 3.13 QDD285 pKa = 3.76 CPTRR289 pKa = 11.84 QYY291 pKa = 11.33 PRR293 pKa = 11.84 YY294 pKa = 8.71 LHH296 pKa = 6.72 NGQTPDD302 pKa = 3.18 KK303 pKa = 10.16 LANTFSTILCKK314 pKa = 9.41 EE315 pKa = 3.87 TGYY318 pKa = 10.4 IFPSFATLSAMGAPWAFPPQQKK340 pKa = 9.67 PVGRR344 pKa = 11.84 GSFNKK349 pKa = 10.14 HH350 pKa = 4.65 KK351 pKa = 10.43 MKK353 pKa = 10.6 GRR355 pKa = 11.84 NDD357 pKa = 3.89 PLKK360 pKa = 10.29 GPWMTLLPKK369 pKa = 10.32 NICNVSEE376 pKa = 4.16 NEE378 pKa = 4.18 MVGGDD383 pKa = 3.99 LNTIIGTLFLAQGSNHH399 pKa = 4.21 YY400 pKa = 6.7 TTYY403 pKa = 11.57 KK404 pKa = 10.12 FMTMQDD410 pKa = 3.34 TVQPLIMQTRR420 pKa = 11.84 AWAAVKK426 pKa = 9.98 IQSRR430 pKa = 11.84 WTLANTRR437 pKa = 11.84 RR438 pKa = 11.84 PYY440 pKa = 10.42 RR441 pKa = 11.84 WDD443 pKa = 3.49 VAWSNTTGKK452 pKa = 10.24 FPQQ455 pKa = 3.75
Molecular weight: 52.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 9.955
IPC_protein 10.804
Toseland 10.701
ProMoST 10.482
Dawson 10.833
Bjellqvist 10.584
Wikipedia 11.067
Rodwell 11.008
Grimsley 10.891
Solomon 10.935
Lehninger 10.891
Nozaki 10.672
DTASelect 10.57
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.76
Patrickios 10.657
IPC_peptide 10.935
IPC2_peptide 9.604
IPC2.peptide.svr19 8.298
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
869
90
455
217.3
24.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.674 ± 1.619
1.381 ± 0.387
4.948 ± 1.798
3.567 ± 0.92
3.567 ± 0.311
5.639 ± 0.603
2.647 ± 0.992
4.948 ± 0.348
4.488 ± 0.454
7.595 ± 0.948
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.956 ± 0.68
4.488 ± 0.655
8.861 ± 0.453
4.948 ± 0.825
10.127 ± 1.011
7.02 ± 1.211
8.285 ± 0.891
3.337 ± 0.761
2.186 ± 0.772
3.337 ± 0.811
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here