Panax notoginseng virus B
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z4PGW3|A0A2Z4PGW3_9VIRU RNA-directed RNA polymerase OS=Panax notoginseng virus B OX=2248770 PE=3 SV=1
MM1 pKa = 8.06 DD2 pKa = 4.56 SFVKK6 pKa = 10.55 QFFTEE11 pKa = 4.5 FDD13 pKa = 3.7 SPGVDD18 pKa = 4.25 FNFDD22 pKa = 3.6 SDD24 pKa = 4.69 LKK26 pKa = 7.94 THH28 pKa = 6.63 TYY30 pKa = 8.2 MGCGLRR36 pKa = 11.84 VYY38 pKa = 10.18 DD39 pKa = 3.44 GHH41 pKa = 7.87 INQSRR46 pKa = 11.84 HH47 pKa = 3.57 FHH49 pKa = 6.71 AFQPMTPLGYY59 pKa = 10.23 YY60 pKa = 10.12 DD61 pKa = 4.73 CEE63 pKa = 4.36 VTGGGSSLDD72 pKa = 4.19 GITKK76 pKa = 10.44 NYY78 pKa = 7.14 LTPEE82 pKa = 3.98 GTVNLVTVADD92 pKa = 4.12 TLKK95 pKa = 10.8 GSSGLQTAMLSAHH108 pKa = 6.03 VSHH111 pKa = 6.18 MQHH114 pKa = 6.46 WSWADD119 pKa = 3.15 NHH121 pKa = 6.69 VGLLFNMFRR130 pKa = 11.84 YY131 pKa = 10.05 VLIEE135 pKa = 4.38 KK136 pKa = 9.92 IRR138 pKa = 11.84 EE139 pKa = 4.15 CAGSLDD145 pKa = 4.0 GKK147 pKa = 10.69 LGLYY151 pKa = 10.27 EE152 pKa = 5.11 DD153 pKa = 4.08 GHH155 pKa = 8.16 VGIDD159 pKa = 3.31 MDD161 pKa = 4.63 QFWEE165 pKa = 4.78 DD166 pKa = 4.62 EE167 pKa = 4.22 YY168 pKa = 11.73 CEE170 pKa = 5.05 DD171 pKa = 3.64 GGARR175 pKa = 11.84 KK176 pKa = 8.74 WPGGDD181 pKa = 2.9 VDD183 pKa = 5.49 ASYY186 pKa = 11.25 PDD188 pKa = 3.91 YY189 pKa = 11.36 LRR191 pKa = 11.84 LTNTTPSTGDD201 pKa = 2.83 WALDD205 pKa = 3.43 LRR207 pKa = 11.84 GMNARR212 pKa = 11.84 DD213 pKa = 3.46 ARR215 pKa = 11.84 FVLLMMGAWKK225 pKa = 10.15 RR226 pKa = 11.84 RR227 pKa = 11.84 SRR229 pKa = 11.84 LRR231 pKa = 11.84 LDD233 pKa = 3.38 CTTKK237 pKa = 10.77 KK238 pKa = 10.8 LCDD241 pKa = 3.43 CVLYY245 pKa = 10.51 RR246 pKa = 11.84 SSVEE250 pKa = 3.95 VANITDD256 pKa = 4.5 WIVDD260 pKa = 3.63 KK261 pKa = 10.95 EE262 pKa = 4.17 KK263 pKa = 11.22 GIVPPTLSSSEE274 pKa = 3.47 AWQALKK280 pKa = 10.5 FYY282 pKa = 8.93 VTQNRR287 pKa = 11.84 LFDD290 pKa = 4.06 HH291 pKa = 7.0 FSATLYY297 pKa = 10.45 IIATMMYY304 pKa = 9.52 QFVPATAEE312 pKa = 3.76 ATNWLRR318 pKa = 11.84 LKK320 pKa = 8.83 WTVNIPTFRR329 pKa = 11.84 SVRR332 pKa = 11.84 GRR334 pKa = 11.84 YY335 pKa = 8.47 EE336 pKa = 3.67 FLNEE340 pKa = 4.17 GEE342 pKa = 4.36 PALLSHH348 pKa = 7.09 RR349 pKa = 11.84 CLNEE353 pKa = 3.06 WGYY356 pKa = 11.01 INGKK360 pKa = 6.53 MEE362 pKa = 4.51 KK363 pKa = 9.35 VNLMALVFSQAYY375 pKa = 6.63 HH376 pKa = 6.12 TGLAVRR382 pKa = 11.84 GVRR385 pKa = 11.84 KK386 pKa = 9.83 GLEE389 pKa = 3.98 LHH391 pKa = 7.07 PDD393 pKa = 4.1 DD394 pKa = 5.02 IFSSEE399 pKa = 3.31 IDD401 pKa = 3.97 FYY403 pKa = 11.35 TSANFISAAASEE415 pKa = 4.55 ATRR418 pKa = 11.84 TDD420 pKa = 3.11 TPLPGMVGIHH430 pKa = 5.71 FVVNDD435 pKa = 3.9 DD436 pKa = 3.72 FDD438 pKa = 5.38 VYY440 pKa = 11.41 DD441 pKa = 3.71 EE442 pKa = 4.0 NRR444 pKa = 11.84 RR445 pKa = 11.84 IEE447 pKa = 4.05 TRR449 pKa = 11.84 EE450 pKa = 3.65 NDD452 pKa = 3.95 DD453 pKa = 3.38 EE454 pKa = 5.66 HH455 pKa = 8.68 LDD457 pKa = 4.21 DD458 pKa = 4.13 YY459 pKa = 10.7 HH460 pKa = 5.99 TSKK463 pKa = 11.3 DD464 pKa = 3.41 KK465 pKa = 11.53 VSVKK469 pKa = 10.35 KK470 pKa = 10.97 GSTLLVDD477 pKa = 4.54 VNDD480 pKa = 4.1 STLSRR485 pKa = 11.84 AALDD489 pKa = 4.77 LISKK493 pKa = 9.91 KK494 pKa = 10.33 IKK496 pKa = 9.48 TLMKK500 pKa = 9.96 GVRR503 pKa = 11.84 AKK505 pKa = 11.13 LEE507 pKa = 4.33 DD508 pKa = 4.12 DD509 pKa = 3.69 VDD511 pKa = 4.75 FEE513 pKa = 4.62 LTDD516 pKa = 3.71 EE517 pKa = 4.54 EE518 pKa = 5.13 KK519 pKa = 10.78 LAQEE523 pKa = 4.03 GKK525 pKa = 9.93 MYY527 pKa = 9.56 VTLAADD533 pKa = 3.68 EE534 pKa = 4.15 QEE536 pKa = 4.16 QMEE539 pKa = 4.7 EE540 pKa = 4.09 KK541 pKa = 10.87 VFVHH545 pKa = 5.78 MPWYY549 pKa = 8.26 TFPGVPTMILPLSPFPYY566 pKa = 8.96 NTPFNMKK573 pKa = 9.91 GRR575 pKa = 11.84 ITTDD579 pKa = 2.15 IGKK582 pKa = 9.54 VDD584 pKa = 3.38 RR585 pKa = 11.84 RR586 pKa = 11.84 GLRR589 pKa = 11.84 LVSKK593 pKa = 7.45 TAWEE597 pKa = 4.35 VANLMRR603 pKa = 11.84 LCGYY607 pKa = 10.22 DD608 pKa = 3.06 SRR610 pKa = 11.84 FRR612 pKa = 11.84 DD613 pKa = 3.52 GGEE616 pKa = 3.5 IAGAGTYY623 pKa = 9.04 FSPNDD628 pKa = 3.76 ANMVWPVLQVPDD640 pKa = 3.97 AQDD643 pKa = 3.78 DD644 pKa = 4.18 EE645 pKa = 5.47 VILTGQIDD653 pKa = 3.76 RR654 pKa = 11.84 EE655 pKa = 4.29 AQFIDD660 pKa = 4.51 LPPMFNRR667 pKa = 11.84 FFIGKK672 pKa = 8.17 EE673 pKa = 3.62 VEE675 pKa = 4.03 YY676 pKa = 10.0 SIKK679 pKa = 10.37 VIRR682 pKa = 11.84 RR683 pKa = 11.84 GTATSFVDD691 pKa = 3.2 NRR693 pKa = 11.84 KK694 pKa = 9.8 DD695 pKa = 3.37 VAEE698 pKa = 4.08 WGGAVTLTRR707 pKa = 11.84 GVTVSYY713 pKa = 10.52 NVPEE717 pKa = 4.23 NVSKK721 pKa = 10.78 LRR723 pKa = 11.84 AYY725 pKa = 9.88 ISRR728 pKa = 11.84 NEE730 pKa = 3.87 TGFRR734 pKa = 11.84 FVDD737 pKa = 3.36 NAQAGVIPMPEE748 pKa = 4.53 PDD750 pKa = 4.5 LNAQGVAGVV759 pKa = 3.57
Molecular weight: 85.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.035
IPC2_protein 5.041
IPC_protein 5.041
Toseland 4.991
ProMoST 5.194
Dawson 5.054
Bjellqvist 5.181
Wikipedia 4.952
Rodwell 4.952
Grimsley 4.914
Solomon 5.054
Lehninger 5.016
Nozaki 5.181
DTASelect 5.372
Thurlkill 5.003
EMBOSS 5.003
Sillero 5.245
Patrickios 4.05
IPC_peptide 5.054
IPC2_peptide 5.232
IPC2.peptide.svr19 5.204
Protein with the highest isoelectric point:
>tr|A0A2Z4PGW3|A0A2Z4PGW3_9VIRU RNA-directed RNA polymerase OS=Panax notoginseng virus B OX=2248770 PE=3 SV=1
MM1 pKa = 7.09 VRR3 pKa = 11.84 RR4 pKa = 11.84 AMSVAYY10 pKa = 10.17 SQVDD14 pKa = 3.47 QYY16 pKa = 12.0 DD17 pKa = 3.58 YY18 pKa = 11.79 SDD20 pKa = 3.13 WQLFRR25 pKa = 11.84 FLRR28 pKa = 11.84 RR29 pKa = 11.84 VFDD32 pKa = 3.56 VDD34 pKa = 3.46 RR35 pKa = 11.84 KK36 pKa = 10.5 LITHH40 pKa = 7.2 ARR42 pKa = 11.84 TGSGPVEE49 pKa = 4.22 GEE51 pKa = 4.01 FPRR54 pKa = 11.84 AAITGEE60 pKa = 3.91 HH61 pKa = 5.97 HH62 pKa = 4.7 THH64 pKa = 6.33 FRR66 pKa = 11.84 PEE68 pKa = 4.23 EE69 pKa = 3.77 VWEE72 pKa = 3.96 VAKK75 pKa = 10.32 IYY77 pKa = 9.79 KK78 pKa = 10.28 AKK80 pKa = 10.0 LRR82 pKa = 11.84 AMSVVYY88 pKa = 10.62 RR89 pKa = 11.84 NLKK92 pKa = 9.55 KK93 pKa = 10.31 IEE95 pKa = 4.93 GITEE99 pKa = 4.16 AVVSTMFLYY108 pKa = 11.02 VLFARR113 pKa = 11.84 PQIAYY118 pKa = 10.1 LFACSRR124 pKa = 11.84 RR125 pKa = 11.84 IWASKK130 pKa = 10.84 DD131 pKa = 3.11 VGQLANVLKK140 pKa = 10.58 EE141 pKa = 3.37 ISTPLKK147 pKa = 10.35 SVQNYY152 pKa = 7.34 EE153 pKa = 4.21 LSDD156 pKa = 3.47 VTQLFEE162 pKa = 4.52 LQCLVNRR169 pKa = 11.84 GIGEE173 pKa = 4.0 VDD175 pKa = 2.88 WNKK178 pKa = 9.66 EE179 pKa = 3.5 RR180 pKa = 11.84 RR181 pKa = 11.84 NRR183 pKa = 11.84 EE184 pKa = 3.72 CPNVVKK190 pKa = 10.86 VDD192 pKa = 3.54 VVDD195 pKa = 4.35 VYY197 pKa = 11.48 KK198 pKa = 10.73 EE199 pKa = 3.93 AVDD202 pKa = 3.56 MFRR205 pKa = 11.84 MGVSHH210 pKa = 6.94 GYY212 pKa = 10.06 KK213 pKa = 9.16 YY214 pKa = 11.47 ARR216 pKa = 11.84 MDD218 pKa = 3.35 LKK220 pKa = 10.98 KK221 pKa = 10.36 YY222 pKa = 10.23 IKK224 pKa = 10.33 SRR226 pKa = 11.84 WEE228 pKa = 3.78 WVPSGSVHH236 pKa = 5.32 SQYY239 pKa = 10.6 IEE241 pKa = 3.94 DD242 pKa = 3.44 QHH244 pKa = 7.17 FIKK247 pKa = 9.43 TDD249 pKa = 2.78 HH250 pKa = 4.95 MHH252 pKa = 5.59 RR253 pKa = 11.84 TKK255 pKa = 10.62 FVTLNMMKK263 pKa = 9.56 TEE265 pKa = 3.89 HH266 pKa = 6.36 LRR268 pKa = 11.84 RR269 pKa = 11.84 MFQRR273 pKa = 11.84 RR274 pKa = 11.84 KK275 pKa = 9.8 EE276 pKa = 3.99 IQAWASVKK284 pKa = 10.46 YY285 pKa = 9.66 EE286 pKa = 3.86 WAKK289 pKa = 10.25 QRR291 pKa = 11.84 AIYY294 pKa = 10.09 GVDD297 pKa = 3.36 LTSSVITNFAMFRR310 pKa = 11.84 CEE312 pKa = 3.74 EE313 pKa = 4.21 VFKK316 pKa = 11.14 HH317 pKa = 5.86 RR318 pKa = 11.84 FPVGEE323 pKa = 3.82 EE324 pKa = 3.53 AAASRR329 pKa = 11.84 VHH331 pKa = 6.42 KK332 pKa = 9.57 RR333 pKa = 11.84 LKK335 pKa = 10.36 MMLDD339 pKa = 3.72 GNEE342 pKa = 4.16 SFCYY346 pKa = 10.74 DD347 pKa = 3.41 FDD349 pKa = 5.14 DD350 pKa = 5.86 FNAQHH355 pKa = 5.87 STEE358 pKa = 3.87 SMYY361 pKa = 11.38 AVLQAYY367 pKa = 10.6 LDD369 pKa = 3.83 VFKK372 pKa = 11.5 GNMSDD377 pKa = 4.05 DD378 pKa = 3.6 QVEE381 pKa = 4.01 AMKK384 pKa = 10.06 WVRR387 pKa = 11.84 DD388 pKa = 3.8 SILSVKK394 pKa = 8.96 VHH396 pKa = 6.03 NNEE399 pKa = 3.8 KK400 pKa = 10.54 NRR402 pKa = 11.84 DD403 pKa = 3.37 EE404 pKa = 4.32 MYY406 pKa = 9.6 STNGTLLSGWRR417 pKa = 11.84 LTTFMNTALNYY428 pKa = 9.79 IYY430 pKa = 10.46 FKK432 pKa = 10.92 LAGAFDD438 pKa = 3.58 IAGVKK443 pKa = 10.5 DD444 pKa = 3.91 SVHH447 pKa = 6.57 NGDD450 pKa = 4.68 DD451 pKa = 3.43 VLVAIKK457 pKa = 9.97 DD458 pKa = 3.81 LKK460 pKa = 10.03 SACSIHH466 pKa = 6.44 QAMAKK471 pKa = 9.75 INARR475 pKa = 11.84 AQATKK480 pKa = 10.86 CNIFSVGEE488 pKa = 3.86 FLRR491 pKa = 11.84 VEE493 pKa = 4.1 HH494 pKa = 6.89 KK495 pKa = 10.16 ISKK498 pKa = 10.16 EE499 pKa = 3.82 EE500 pKa = 3.96 GLGAQYY506 pKa = 9.05 LTRR509 pKa = 11.84 AAATLAHH516 pKa = 6.4 SRR518 pKa = 11.84 IEE520 pKa = 4.27 SQEE523 pKa = 3.57 PTRR526 pKa = 11.84 LVDD529 pKa = 3.96 SIKK532 pKa = 11.09 GMVTRR537 pKa = 11.84 CEE539 pKa = 4.14 EE540 pKa = 3.77 ITARR544 pKa = 11.84 SYY546 pKa = 12.27 DD547 pKa = 3.78 MVEE550 pKa = 4.0 VAYY553 pKa = 10.4 HH554 pKa = 7.14 LLTEE558 pKa = 4.45 SVTRR562 pKa = 11.84 LCGIFGVKK570 pKa = 10.02 KK571 pKa = 9.32 EE572 pKa = 4.81 DD573 pKa = 3.4 GMKK576 pKa = 8.98 VTRR579 pKa = 11.84 SHH581 pKa = 6.77 LLVGGASEE589 pKa = 4.24 TFEE592 pKa = 4.94 GIVDD596 pKa = 3.58 EE597 pKa = 4.83 TIEE600 pKa = 4.26 EE601 pKa = 4.12 EE602 pKa = 4.6 VLIDD606 pKa = 3.38 DD607 pKa = 4.5 TRR609 pKa = 11.84 ITEE612 pKa = 3.96 EE613 pKa = 4.09 NRR615 pKa = 11.84 DD616 pKa = 3.18 KK617 pKa = 11.07 RR618 pKa = 11.84 ATIRR622 pKa = 11.84 EE623 pKa = 4.48 LMPGINDD630 pKa = 3.3 YY631 pKa = 11.75 AEE633 pKa = 4.18 ILEE636 pKa = 4.34 TQYY639 pKa = 11.86 GEE641 pKa = 4.96 FMPLDD646 pKa = 4.0 DD647 pKa = 4.96 VRR649 pKa = 11.84 KK650 pKa = 9.27 KK651 pKa = 10.88 VINATHH657 pKa = 6.4 RR658 pKa = 11.84 QLEE661 pKa = 4.32 ITRR664 pKa = 11.84 GTNLKK669 pKa = 8.64 ITDD672 pKa = 3.23 VRR674 pKa = 11.84 HH675 pKa = 4.1 TVKK678 pKa = 10.95 YY679 pKa = 10.12 KK680 pKa = 10.43 FGRR683 pKa = 11.84 ALFRR687 pKa = 11.84 MYY689 pKa = 10.45 KK690 pKa = 10.24 DD691 pKa = 3.45 KK692 pKa = 11.74 VNIPYY697 pKa = 9.74 VEE699 pKa = 4.11 KK700 pKa = 10.9 ARR702 pKa = 11.84 FLGIPPIAMLSHH714 pKa = 7.13 SSMKK718 pKa = 10.26 FVTKK722 pKa = 10.64 LISSVKK728 pKa = 10.58 DD729 pKa = 2.94 VDD731 pKa = 3.66 YY732 pKa = 10.68 TLRR735 pKa = 11.84 ALLL738 pKa = 4.1
Molecular weight: 85.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.033
IPC2_protein 8.141
IPC_protein 8.009
Toseland 8.39
ProMoST 8.551
Dawson 8.887
Bjellqvist 8.902
Wikipedia 8.975
Rodwell 9.004
Grimsley 8.653
Solomon 8.96
Lehninger 8.916
Nozaki 8.99
DTASelect 8.756
Thurlkill 8.843
EMBOSS 9.048
Sillero 9.048
Patrickios 4.596
IPC_peptide 8.946
IPC2_peptide 7.6
IPC2.peptide.svr19 7.629
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1497
738
759
748.5
85.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.947 ± 0.257
1.202 ± 0.012
7.014 ± 0.826
6.546 ± 0.536
4.743 ± 0.189
6.146 ± 0.976
2.672 ± 0.309
4.81 ± 0.519
5.945 ± 0.86
7.882 ± 0.393
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.54 ± 0.176
3.941 ± 0.291
3.006 ± 0.772
2.739 ± 0.169
6.613 ± 0.584
5.945 ± 0.106
6.279 ± 0.409
8.484 ± 0.414
1.67 ± 0.219
3.874 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here