Streptococcus satellite phage Javan644

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZTW5|A0A4D5ZTW5_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan644 OX=2558801 GN=JavanS644_0012 PE=4 SV=1
MM1 pKa = 7.72IKK3 pKa = 10.02QLSDD7 pKa = 3.29TPKK10 pKa = 9.96TYY12 pKa = 10.87QFTYY16 pKa = 10.02KK17 pKa = 10.08CQNAQEE23 pKa = 4.27ISNALIGYY31 pKa = 7.6MIGTYY36 pKa = 9.56DD37 pKa = 3.22QSAIEE42 pKa = 3.79ITYY45 pKa = 10.09IGNGNLKK52 pKa = 10.53AEE54 pKa = 4.29YY55 pKa = 9.75TSDD58 pKa = 3.51EE59 pKa = 4.37DD60 pKa = 3.52LTEE63 pKa = 4.1SFEE66 pKa = 5.39RR67 pKa = 11.84ITKK70 pKa = 10.48SLDD73 pKa = 3.07HH74 pKa = 6.72TDD76 pKa = 4.11DD77 pKa = 4.16YY78 pKa = 11.88EE79 pKa = 5.82DD80 pKa = 4.63YY81 pKa = 11.15EE82 pKa = 5.48DD83 pKa = 5.85DD84 pKa = 4.25FEE86 pKa = 5.5EE87 pKa = 5.98DD88 pKa = 3.34EE89 pKa = 4.46LTIKK93 pKa = 10.69SDD95 pKa = 3.44LNTYY99 pKa = 7.45TALIGSFNTLGEE111 pKa = 4.17AQAFTEE117 pKa = 4.58NLDD120 pKa = 4.44DD121 pKa = 6.72DD122 pKa = 4.55LTNGNNFIYY131 pKa = 9.99EE132 pKa = 4.13QTPDD136 pKa = 3.32TVALYY141 pKa = 9.61VSPQDD146 pKa = 3.67TIANSTIQKK155 pKa = 10.38LEE157 pKa = 3.71EE158 pKa = 4.44AYY160 pKa = 10.47VKK162 pKa = 10.8HH163 pKa = 6.02EE164 pKa = 4.35GEE166 pKa = 4.34YY167 pKa = 10.98EE168 pKa = 4.01PDD170 pKa = 3.39TFRR173 pKa = 11.84LSYY176 pKa = 10.96HH177 pKa = 6.43DD178 pKa = 4.11MRR180 pKa = 11.84VQQLQSLDD188 pKa = 3.68KK189 pKa = 10.41DD190 pKa = 3.77TLINDD195 pKa = 4.76TINYY199 pKa = 8.89EE200 pKa = 4.56LEE202 pKa = 4.03LLDD205 pKa = 3.62YY206 pKa = 10.48TDD208 pKa = 5.22RR209 pKa = 11.84LLSDD213 pKa = 3.87EE214 pKa = 4.85PLPLDD219 pKa = 3.83SLHH222 pKa = 6.82GYY224 pKa = 7.32EE225 pKa = 4.39TIEE228 pKa = 3.94LLGDD232 pKa = 3.62EE233 pKa = 5.17VIDD236 pKa = 4.61LVKK239 pKa = 10.79EE240 pKa = 3.7LDD242 pKa = 3.72TNKK245 pKa = 10.15KK246 pKa = 9.69FDD248 pKa = 4.77GIHH251 pKa = 7.14DD252 pKa = 4.37YY253 pKa = 10.92IIGEE257 pKa = 4.1

Molecular weight: 29.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZSZ8|A0A4D5ZSZ8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan644 OX=2558801 GN=JavanS644_0009 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.27LYY4 pKa = 10.3IIIIKK9 pKa = 9.38NVKK12 pKa = 9.3NVSSALEE19 pKa = 4.02LTKK22 pKa = 10.22MLGGRR27 pKa = 11.84QMEE30 pKa = 4.25ITIDD34 pKa = 3.24QAKK37 pKa = 10.27AIRR40 pKa = 11.84RR41 pKa = 11.84KK42 pKa = 9.15QADD45 pKa = 3.4MQLTDD50 pKa = 3.38KK51 pKa = 11.38

Molecular weight: 5.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

2136

38

485

164.3

19.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.839 ± 0.273

0.468 ± 0.118

6.554 ± 0.867

8.895 ± 0.888

3.933 ± 0.379

4.588 ± 0.409

1.732 ± 0.268

8.661 ± 0.455

9.551 ± 0.879

10.487 ± 0.566

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.481 ± 0.407

6.976 ± 0.55

2.996 ± 0.377

3.137 ± 0.401

4.26 ± 0.608

5.243 ± 0.685

6.273 ± 0.695

4.213 ± 0.436

0.843 ± 0.236

4.869 ± 0.687
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski