Tortoise microvirus 99

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 5.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W768|A0A4P8W768_9VIRU Uncharacterized protein OS=Tortoise microvirus 99 OX=2583208 PE=4 SV=1
MM1 pKa = 7.61EE2 pKa = 5.18CFMYY6 pKa = 10.61NVDD9 pKa = 4.12MSNGSIIPTAYY20 pKa = 10.09GSCDD24 pKa = 3.28TLTVIFPRR32 pKa = 11.84ALTDD36 pKa = 4.93AIDD39 pKa = 3.48ILLVDD44 pKa = 4.84DD45 pKa = 5.04TMTLPDD51 pKa = 5.7FIRR54 pKa = 11.84LACAEE59 pKa = 4.15YY60 pKa = 10.91LLSFKK65 pKa = 10.77RR66 pKa = 11.84GFF68 pKa = 3.4

Molecular weight: 7.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8WA84|A0A4P8WA84_9VIRU Uncharacterized protein OS=Tortoise microvirus 99 OX=2583208 PE=4 SV=1
MM1 pKa = 7.56SCRR4 pKa = 11.84LTYY7 pKa = 10.42QSYY10 pKa = 11.28LLFCCKK16 pKa = 10.02RR17 pKa = 11.84EE18 pKa = 4.95LFDD21 pKa = 4.33CYY23 pKa = 10.61QRR25 pKa = 11.84GLGASFLTFTYY36 pKa = 11.05NDD38 pKa = 3.55DD39 pKa = 4.02CLPLNGSLDD48 pKa = 3.36KK49 pKa = 11.15RR50 pKa = 11.84SFQNLLKK57 pKa = 10.35RR58 pKa = 11.84VRR60 pKa = 11.84RR61 pKa = 11.84NKK63 pKa = 10.05GLPSFKK69 pKa = 10.62YY70 pKa = 9.65LACGEE75 pKa = 4.35YY76 pKa = 10.54GDD78 pKa = 4.17KK79 pKa = 10.63FNRR82 pKa = 11.84AHH84 pKa = 5.12YY85 pKa = 9.49HH86 pKa = 5.46AVFFGLTDD94 pKa = 3.3VLAKK98 pKa = 10.51QFVASHH104 pKa = 6.58WLNGYY109 pKa = 9.47VKK111 pKa = 10.6VEE113 pKa = 4.1ALRR116 pKa = 11.84SSAGLRR122 pKa = 11.84YY123 pKa = 10.1VLDD126 pKa = 3.97YY127 pKa = 10.64CCKK130 pKa = 10.57SINGDD135 pKa = 3.91LAVEE139 pKa = 4.84LYY141 pKa = 10.66DD142 pKa = 3.52NQMKK146 pKa = 9.85EE147 pKa = 4.24RR148 pKa = 11.84PFLSHH153 pKa = 5.05STRR156 pKa = 11.84MGFDD160 pKa = 2.67WLVRR164 pKa = 11.84NMEE167 pKa = 5.08DD168 pKa = 2.69IVANGFYY175 pKa = 10.4YY176 pKa = 10.51RR177 pKa = 11.84EE178 pKa = 4.2AGKK181 pKa = 10.19LLPLPKK187 pKa = 9.94YY188 pKa = 10.43FRR190 pKa = 11.84DD191 pKa = 3.28IYY193 pKa = 10.82DD194 pKa = 3.07IYY196 pKa = 10.81KK197 pKa = 9.0TFDD200 pKa = 2.9VSRR203 pKa = 11.84VLRR206 pKa = 11.84SLDD209 pKa = 3.27THH211 pKa = 6.77AKK213 pKa = 10.26RR214 pKa = 11.84MGFPDD219 pKa = 3.33IYY221 pKa = 10.74SYY223 pKa = 11.66SDD225 pKa = 3.36YY226 pKa = 10.36VTPLRR231 pKa = 11.84EE232 pKa = 3.9KK233 pKa = 10.75EE234 pKa = 4.39LIAKK238 pKa = 9.66SRR240 pKa = 11.84LAGRR244 pKa = 11.84PVDD247 pKa = 3.85DD248 pKa = 4.7SNFVSSFYY256 pKa = 10.67VPRR259 pKa = 11.84VGTRR263 pKa = 11.84SLAKK267 pKa = 10.17SALEE271 pKa = 3.91RR272 pKa = 4.16

Molecular weight: 31.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1791

68

606

223.9

25.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.705 ± 0.997

1.675 ± 0.599

6.253 ± 0.652

4.858 ± 1.061

5.528 ± 0.659

5.248 ± 0.505

1.731 ± 0.339

5.081 ± 0.953

4.076 ± 0.903

9.436 ± 0.619

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.442

4.467 ± 0.551

4.299 ± 0.492

3.797 ± 1.271

5.918 ± 0.529

10.441 ± 1.096

5.583 ± 1.031

4.69 ± 0.589

1.005 ± 0.271

5.583 ± 0.628
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski