Lutibaculum baratangense AMV1
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4164 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V4R2N0|V4R2N0_9RHIZ Uncharacterized protein OS=Lutibaculum baratangense AMV1 OX=631454 GN=N177_1071 PE=4 SV=1
MM1 pKa = 7.05 NRR3 pKa = 11.84 FVRR6 pKa = 11.84 TLTLGAAVAVLATAANAADD25 pKa = 3.36 APYY28 pKa = 10.04 YY29 pKa = 10.28 DD30 pKa = 4.18 KK31 pKa = 11.58 NVVVSPAPVSVTDD44 pKa = 3.29 WTGMYY49 pKa = 10.14 VGVFGGYY56 pKa = 10.0 GGGDD60 pKa = 3.24 FEE62 pKa = 5.57 YY63 pKa = 9.43 PFSVGSPALLSGQVDD78 pKa = 3.8 MSAGGFVGGAQIGFDD93 pKa = 3.65 WQASDD98 pKa = 3.5 AFVVGVVADD107 pKa = 4.15 IAWSDD112 pKa = 3.15 ISDD115 pKa = 3.7 EE116 pKa = 4.41 VSVSLAAATDD126 pKa = 4.79 PITRR130 pKa = 11.84 VNGSLGTDD138 pKa = 3.58 LNYY141 pKa = 10.6 FGTIRR146 pKa = 11.84 ARR148 pKa = 11.84 AGWLVTPDD156 pKa = 3.07 ALLYY160 pKa = 8.53 VTGGAAYY167 pKa = 10.26 GEE169 pKa = 4.56 TEE171 pKa = 4.17 SALSLSASGAVDD183 pKa = 3.85 GSFGASTSDD192 pKa = 3.66 TDD194 pKa = 3.53 WGWTVGAGMEE204 pKa = 3.98 YY205 pKa = 10.29 RR206 pKa = 11.84 ITEE209 pKa = 4.02 NVSFEE214 pKa = 4.19 TEE216 pKa = 3.96 YY217 pKa = 11.13 LYY219 pKa = 11.48 VDD221 pKa = 4.62 LGSKK225 pKa = 8.04 TLYY228 pKa = 10.73 SGDD231 pKa = 3.89 VFDD234 pKa = 6.26 LPARR238 pKa = 11.84 LDD240 pKa = 3.93 LDD242 pKa = 3.72 TSFHH246 pKa = 5.28 VVRR249 pKa = 11.84 AGVNFKK255 pKa = 11.3 FNMFF259 pKa = 3.72
Molecular weight: 27.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.846
IPC_protein 3.859
Toseland 3.63
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.821
Rodwell 3.681
Grimsley 3.541
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.253
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.986
Patrickios 1.914
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.867
Protein with the highest isoelectric point:
>tr|V4RAY5|V4RAY5_9RHIZ Transcription regulator OS=Lutibaculum baratangense AMV1 OX=631454 GN=N177_3700 PE=4 SV=1
MM1 pKa = 7.41 AAALGTGSISRR12 pKa = 11.84 LRR14 pKa = 11.84 TLRR17 pKa = 11.84 ARR19 pKa = 11.84 ATLFLIRR26 pKa = 11.84 NKK28 pKa = 9.96 FSNRR32 pKa = 11.84 KK33 pKa = 8.35 RR34 pKa = 11.84 VRR36 pKa = 11.84 AGWRR40 pKa = 11.84 HH41 pKa = 3.76 GHH43 pKa = 6.16 GPSSPPAGTRR53 pKa = 11.84 RR54 pKa = 11.84 PSAQQ58 pKa = 2.93
Molecular weight: 6.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.522
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.574
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.31
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.2
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4164
0
4164
1287530
37
2455
309.2
33.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.56 ± 0.056
0.818 ± 0.012
5.596 ± 0.033
6.739 ± 0.039
3.621 ± 0.026
9.014 ± 0.036
2.076 ± 0.018
4.714 ± 0.032
2.509 ± 0.028
10.184 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.473 ± 0.018
2.135 ± 0.018
5.342 ± 0.03
2.809 ± 0.023
7.963 ± 0.044
5.114 ± 0.026
5.06 ± 0.028
7.853 ± 0.033
1.318 ± 0.017
2.101 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here