Lake Sarah-associated circular virus-28

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQN0|A0A140AQN0_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-28 OX=1685755 PE=4 SV=1
MM1 pKa = 7.92ASRR4 pKa = 11.84KK5 pKa = 9.77YY6 pKa = 10.16KK7 pKa = 10.63FCASCFNYY15 pKa = 10.07CDD17 pKa = 3.91SCDD20 pKa = 3.67CLGNLEE26 pKa = 4.17DD27 pKa = 4.84HH28 pKa = 6.03EE29 pKa = 5.29HH30 pKa = 6.57INTFEE35 pKa = 4.25RR36 pKa = 11.84PGKK39 pKa = 10.96GNDD42 pKa = 3.57DD43 pKa = 3.98DD44 pKa = 5.82EE45 pKa = 6.0EE46 pKa = 5.96IDD48 pKa = 5.92DD49 pKa = 5.24EE50 pKa = 5.21EE51 pKa = 5.84EE52 pKa = 4.2DD53 pKa = 4.2DD54 pKa = 4.49NRR56 pKa = 11.84NKK58 pKa = 10.89GGGEE62 pKa = 3.55VWYY65 pKa = 10.0FRR67 pKa = 11.84AQVDD71 pKa = 4.21LTLMAGGNMLLGALMTMEE89 pKa = 4.92EE90 pKa = 4.65MWTDD94 pKa = 3.71IPPDD98 pKa = 3.81NPSLYY103 pKa = 10.75LSIPKK108 pKa = 9.86SRR110 pKa = 11.84FPMDD114 pKa = 3.49PVNIGGSFPNKK125 pKa = 9.53PEE127 pKa = 4.16YY128 pKa = 10.12IKK130 pKa = 10.56PKK132 pKa = 9.64YY133 pKa = 8.52PWEE136 pKa = 3.86IPYY139 pKa = 9.82FQLSYY144 pKa = 10.38PRR146 pKa = 11.84PPEE149 pKa = 4.22KK150 pKa = 10.34SDD152 pKa = 3.53AAIASQAKK160 pKa = 10.2DD161 pKa = 3.52CTKK164 pKa = 10.97DD165 pKa = 3.21CLKK168 pKa = 10.44RR169 pKa = 11.84FSFRR173 pKa = 11.84PVRR176 pKa = 11.84LGICTAKK183 pKa = 10.33CAVIAAGKK191 pKa = 9.87KK192 pKa = 9.83AFNEE196 pKa = 4.31FNVSLEE202 pKa = 4.2DD203 pKa = 3.84IYY205 pKa = 11.54LGVQKK210 pKa = 9.63TINLDD215 pKa = 3.54NLPLYY220 pKa = 10.22IGLIPLIKK228 pKa = 10.23KK229 pKa = 7.08YY230 pKa = 9.44TPPRR234 pKa = 11.84LWNAADD240 pKa = 3.97DD241 pKa = 4.39SVVPGCGFQWVEE253 pKa = 3.46MHH255 pKa = 7.15LIDD258 pKa = 5.74GSDD261 pKa = 3.5PSSDD265 pKa = 3.48FDD267 pKa = 4.65CSGKK271 pKa = 10.51LSSNVDD277 pKa = 3.05GVDD280 pKa = 3.86QFWSDD285 pKa = 3.42NQNGGDD291 pKa = 3.47TSEE294 pKa = 4.28TLKK297 pKa = 10.8DD298 pKa = 3.94YY299 pKa = 11.45YY300 pKa = 10.24PDD302 pKa = 4.23ASDD305 pKa = 3.28PTTDD309 pKa = 2.87VRR311 pKa = 11.84MMMMPHH317 pKa = 6.34VPPPVPPVGPPRR329 pKa = 11.84CTQWALDD336 pKa = 4.06HH337 pKa = 6.49GFCDD341 pKa = 5.39KK342 pKa = 11.38DD343 pKa = 4.21DD344 pKa = 5.17DD345 pKa = 4.63PPPYY349 pKa = 9.99IPPPPTHH356 pKa = 7.09VDD358 pKa = 3.16PPIIVDD364 pKa = 3.75PPIVLPPTGTPPTVLPTYY382 pKa = 10.47DD383 pKa = 2.8ITNNINFPMAVIQYY397 pKa = 9.23NSKK400 pKa = 8.47TRR402 pKa = 11.84ADD404 pKa = 3.66WYY406 pKa = 10.84NSGSTGYY413 pKa = 11.06ADD415 pKa = 4.46DD416 pKa = 3.83RR417 pKa = 11.84QRR419 pKa = 11.84PHH421 pKa = 6.98LNWGAAAIPLKK432 pKa = 10.12TGDD435 pKa = 3.68VIDD438 pKa = 4.52RR439 pKa = 11.84GVTLEE444 pKa = 3.89IPEE447 pKa = 4.17NNYY450 pKa = 10.75EE451 pKa = 4.06YY452 pKa = 11.12VDD454 pKa = 3.57CFFYY458 pKa = 11.36YY459 pKa = 9.49MAHH462 pKa = 6.5RR463 pKa = 11.84PIAGRR468 pKa = 11.84VSMRR472 pKa = 11.84LTYY475 pKa = 10.77VSGPKK480 pKa = 9.59PSGMGFKK487 pKa = 9.88VHH489 pKa = 6.92WDD491 pKa = 3.45NSVKK495 pKa = 10.74PGDD498 pKa = 3.9TQTLRR503 pKa = 11.84GDD505 pKa = 3.32VTTFQFNKK513 pKa = 10.02NMPAGISIHH522 pKa = 5.78SFRR525 pKa = 11.84LTPLHH530 pKa = 6.31VNPPGLQNKK539 pKa = 8.37QEE541 pKa = 4.13FKK543 pKa = 11.44LEE545 pKa = 4.84FFTAWDD551 pKa = 4.0KK552 pKa = 11.33DD553 pKa = 3.97CKK555 pKa = 10.5PVFTLARR562 pKa = 11.84DD563 pKa = 4.1IPWEE567 pKa = 4.14TPYY570 pKa = 11.01GGPPPVFNGIGVVDD584 pKa = 5.33FIGNNNKK591 pKa = 10.07DD592 pKa = 4.29CIAAHH597 pKa = 7.16PIPLNPMVGGVRR609 pKa = 11.84VKK611 pKa = 10.65PPTFSWGNGEE621 pKa = 6.05CIICHH626 pKa = 5.35NQDD629 pKa = 2.99PKK631 pKa = 11.23LPGVAVHH638 pKa = 6.73KK639 pKa = 10.14MNNTGDD645 pKa = 3.64GGITPINSQSYY656 pKa = 7.51FFTWVNPSANGYY668 pKa = 8.27GQGIARR674 pKa = 11.84IDD676 pKa = 3.66GSLGSYY682 pKa = 10.29YY683 pKa = 10.48GACKK687 pKa = 10.26SWTKK691 pKa = 11.09

Molecular weight: 76.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GA16|A0A126GA16_9VIRU Uncharacterized protein OS=Lake Sarah-associated circular virus-28 OX=1685755 PE=4 SV=1
MM1 pKa = 7.27SAVAHH6 pKa = 5.91CAVLRR11 pKa = 11.84NPFSTAVATCKK22 pKa = 10.29IPDD25 pKa = 3.58GSAVLSSSSRR35 pKa = 11.84LSHH38 pKa = 6.26SKK40 pKa = 10.28HH41 pKa = 5.83YY42 pKa = 11.08SCTQNTLCIALYY54 pKa = 10.05PGFQSHH60 pKa = 6.57IFLSGKK66 pKa = 10.02NISQGGGIIGGDD78 pKa = 3.32DD79 pKa = 3.5VANPDD84 pKa = 3.68LQLSSRR90 pKa = 11.84TQKK93 pKa = 10.36EE94 pKa = 4.64GPVGAGGTMTTTQNANAPSKK114 pKa = 10.86YY115 pKa = 10.0RR116 pKa = 11.84IASQGLRR123 pKa = 11.84VSLIEE128 pKa = 4.05NSEE131 pKa = 4.22NNDD134 pKa = 2.55GWFEE138 pKa = 4.72AIRR141 pKa = 11.84IPSAYY146 pKa = 9.73DD147 pKa = 2.9ASDD150 pKa = 3.24FVTLVSTLDD159 pKa = 3.36STSQYY164 pKa = 10.49MGLQKK169 pKa = 10.51DD170 pKa = 3.61KK171 pKa = 11.14KK172 pKa = 10.49IFGGNLGLGTDD183 pKa = 4.03PTSITNWSMNPTYY196 pKa = 9.82ITGKK200 pKa = 9.85LRR202 pKa = 11.84DD203 pKa = 3.85INRR206 pKa = 11.84HH207 pKa = 3.64TFMLHH212 pKa = 6.56RR213 pKa = 11.84EE214 pKa = 3.78NDD216 pKa = 3.15MDD218 pKa = 5.14FVDD221 pKa = 4.15VNNTAEE227 pKa = 4.76FIANGTSVSYY237 pKa = 10.47GKK239 pKa = 10.17SEE241 pKa = 3.87ITRR244 pKa = 11.84AANPSVWWVDD254 pKa = 3.68SNLDD258 pKa = 3.83TILIRR263 pKa = 11.84CFGSIVGSGSGMSVHH278 pKa = 6.08IHH280 pKa = 5.06TVQHH284 pKa = 5.79VEE286 pKa = 4.15EE287 pKa = 4.16QFEE290 pKa = 4.65PNNEE294 pKa = 3.82LARR297 pKa = 11.84YY298 pKa = 7.14MIKK301 pKa = 10.58PPVNTALTTAVMNAIRR317 pKa = 11.84RR318 pKa = 11.84EE319 pKa = 3.99IKK321 pKa = 10.07PSIIRR326 pKa = 11.84APTGGVSVRR335 pKa = 11.84FSRR338 pKa = 11.84TPRR341 pKa = 11.84TYY343 pKa = 10.4RR344 pKa = 11.84RR345 pKa = 11.84RR346 pKa = 11.84KK347 pKa = 7.37SVRR350 pKa = 11.84RR351 pKa = 11.84RR352 pKa = 11.84RR353 pKa = 11.84YY354 pKa = 6.4TT355 pKa = 2.99

Molecular weight: 38.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1362

316

691

454.0

50.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.947 ± 0.58

2.349 ± 0.36

7.122 ± 1.043

4.772 ± 1.252

4.332 ± 0.353

7.269 ± 0.888

2.129 ± 0.147

5.947 ± 0.41

5.653 ± 0.954

5.653 ± 0.105

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.07

5.727 ± 0.59

7.562 ± 2.166

2.863 ± 0.27

5.36 ± 1.241

6.828 ± 1.519

5.8 ± 0.982

6.094 ± 0.207

1.689 ± 0.322

4.258 ± 0.686
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski