Streptococcus satellite phage Javan442
Average proteome isoelectric point is 7.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZNF6|A0A4D5ZNF6_9VIRU Putative DNA-binding phage protein OS=Streptococcus satellite phage Javan442 OX=2558707 GN=JavanS442_0015 PE=4 SV=1
MM1 pKa = 6.88 EE2 pKa = 5.53 TYY4 pKa = 10.93 NFDD7 pKa = 4.58 DD8 pKa = 4.34 KK9 pKa = 11.32 YY10 pKa = 7.27 TTKK13 pKa = 10.45 RR14 pKa = 11.84 VAQLQDD20 pKa = 3.02 MDD22 pKa = 4.08 KK23 pKa = 10.96 QDD25 pKa = 5.84 LINLMAKK32 pKa = 10.35 YY33 pKa = 9.87 EE34 pKa = 4.31 LEE36 pKa = 4.16 LLDD39 pKa = 3.8 YY40 pKa = 11.05 ADD42 pKa = 4.71 RR43 pKa = 11.84 LLSEE47 pKa = 5.02 EE48 pKa = 5.23 PISMDD53 pKa = 3.56 AGTGCGTVQLLGQSVTEE70 pKa = 4.73 LVSQLDD76 pKa = 3.14 KK77 pKa = 11.33 DD78 pKa = 3.72 KK79 pKa = 10.21 EE80 pKa = 4.29 YY81 pKa = 11.16 KK82 pKa = 10.47 GIYY85 pKa = 9.69 SII87 pKa = 5.34
Molecular weight: 9.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.267
IPC2_protein 4.24
IPC_protein 4.151
Toseland 3.961
ProMoST 4.304
Dawson 4.126
Bjellqvist 4.279
Wikipedia 4.05
Rodwell 3.986
Grimsley 3.872
Solomon 4.113
Lehninger 4.075
Nozaki 4.24
DTASelect 4.457
Thurlkill 3.999
EMBOSS 4.062
Sillero 4.266
Patrickios 3.503
IPC_peptide 4.113
IPC2_peptide 4.253
IPC2.peptide.svr19 4.181
Protein with the highest isoelectric point:
>tr|A0A4D5ZQF5|A0A4D5ZQF5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan442 OX=2558707 GN=JavanS442_0008 PE=4 SV=1
MM1 pKa = 7.92 KK2 pKa = 9.25 ITEE5 pKa = 4.18 YY6 pKa = 10.59 KK7 pKa = 9.93 KK8 pKa = 10.55 KK9 pKa = 10.38 NGTIMYY15 pKa = 7.67 RR16 pKa = 11.84 TQVYY20 pKa = 10.49 LGVDD24 pKa = 2.83 KK25 pKa = 11.06 VTGNKK30 pKa = 9.65 ARR32 pKa = 11.84 TSVSGRR38 pKa = 11.84 TKK40 pKa = 10.48 KK41 pKa = 10.48 ALKK44 pKa = 10.75 NNIKK48 pKa = 9.31 LAKK51 pKa = 9.2 MSFGG55 pKa = 3.54
Molecular weight: 6.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.212
IPC2_protein 10.087
IPC_protein 10.262
Toseland 10.862
ProMoST 10.365
Dawson 10.95
Bjellqvist 10.54
Wikipedia 11.067
Rodwell 11.637
Grimsley 10.979
Solomon 10.994
Lehninger 10.979
Nozaki 10.818
DTASelect 10.54
Thurlkill 10.847
EMBOSS 11.242
Sillero 10.862
Patrickios 11.374
IPC_peptide 10.994
IPC2_peptide 9.048
IPC2.peptide.svr19 8.464
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19
0
19
2007
37
320
105.6
12.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.833 ± 0.489
0.997 ± 0.306
5.929 ± 0.482
7.623 ± 0.794
3.687 ± 0.459
4.783 ± 0.427
1.545 ± 0.333
7.623 ± 0.287
10.414 ± 0.798
10.314 ± 0.355
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.79 ± 0.368
5.232 ± 0.489
2.392 ± 0.258
4.534 ± 0.476
5.481 ± 0.437
5.331 ± 0.575
5.63 ± 0.509
4.933 ± 0.386
0.897 ± 0.216
5.032 ± 0.397
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here