Arthrobacter phage Shiba

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TLZ1|A0A5J6TLZ1_9CAUD Uncharacterized protein OS=Arthrobacter phage Shiba OX=2599834 GN=86 PE=4 SV=1
MM1 pKa = 6.72TQQAAITTVDD11 pKa = 3.16NPFDD15 pKa = 3.57VFTQFDD21 pKa = 3.7EE22 pKa = 4.27WNAWDD27 pKa = 4.59LEE29 pKa = 4.43HH30 pKa = 7.66GYY32 pKa = 7.93GTCAYY37 pKa = 8.99LARR40 pKa = 11.84VVRR43 pKa = 11.84SSDD46 pKa = 3.29EE47 pKa = 4.14LSIADD52 pKa = 3.29QDD54 pKa = 3.98LALEE58 pKa = 4.06YY59 pKa = 10.85GIDD62 pKa = 3.56EE63 pKa = 5.2IIRR66 pKa = 11.84EE67 pKa = 4.36DD68 pKa = 3.97VLGLYY73 pKa = 10.38KK74 pKa = 10.34KK75 pKa = 10.61VLADD79 pKa = 3.79KK80 pKa = 10.53EE81 pKa = 4.33DD82 pKa = 3.63VSTSEE87 pKa = 4.09SSEE90 pKa = 3.9

Molecular weight: 10.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TGE0|A0A5J6TGE0_9CAUD Uncharacterized protein OS=Arthrobacter phage Shiba OX=2599834 GN=64 PE=4 SV=1
MM1 pKa = 7.5SAPKK5 pKa = 9.87PSEE8 pKa = 4.21VNTKK12 pKa = 8.89PRR14 pKa = 11.84KK15 pKa = 8.87EE16 pKa = 3.6RR17 pKa = 11.84SAPVRR22 pKa = 11.84NPAFRR27 pKa = 11.84EE28 pKa = 4.13SEE30 pKa = 4.16PLKK33 pKa = 10.5DD34 pKa = 3.55LQRR37 pKa = 11.84RR38 pKa = 11.84LHH40 pKa = 6.56AEE42 pKa = 3.66ARR44 pKa = 11.84NKK46 pKa = 9.87KK47 pKa = 9.78

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

17009

41

1506

197.8

22.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.889 ± 0.446

0.623 ± 0.089

6.073 ± 0.337

6.549 ± 0.378

3.698 ± 0.195

7.496 ± 0.387

1.834 ± 0.177

5.903 ± 0.195

6.408 ± 0.43

8.025 ± 0.388

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.816 ± 0.191

4.503 ± 0.179

4.139 ± 0.219

3.281 ± 0.157

5.168 ± 0.354

5.997 ± 0.241

6.432 ± 0.323

6.961 ± 0.227

1.734 ± 0.18

3.469 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski