Escherichia phage ESSI2_ev129

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Seongnamvirus; Escherichia virus ev129

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653FW57|A0A653FW57_9CAUD Uncharacterized protein OS=Escherichia phage ESSI2_ev129 OX=2695848 PE=4 SV=1
MM1 pKa = 7.41SQTQIQSLTAFFKK14 pKa = 11.14EE15 pKa = 4.29NVPPRR20 pKa = 11.84AMQSFDD26 pKa = 3.63SVLDD30 pKa = 3.58EE31 pKa = 4.46MKK33 pKa = 10.67FIPAAKK39 pKa = 9.95DD40 pKa = 3.34YY41 pKa = 11.64GLGQYY46 pKa = 8.95RR47 pKa = 11.84QAVIRR52 pKa = 11.84YY53 pKa = 7.93DD54 pKa = 4.26AVLSWEE60 pKa = 4.03RR61 pKa = 11.84FPYY64 pKa = 10.09RR65 pKa = 11.84LCPPQLLMSLLAAWLDD81 pKa = 3.77DD82 pKa = 4.24ADD84 pKa = 5.68RR85 pKa = 11.84EE86 pKa = 4.2LLDD89 pKa = 3.74EE90 pKa = 4.72VGLSEE95 pKa = 6.27SEE97 pKa = 4.31PDD99 pKa = 2.89WDD101 pKa = 3.88VSVEE105 pKa = 4.05DD106 pKa = 4.35EE107 pKa = 4.27EE108 pKa = 4.82VATVVLTVPMVEE120 pKa = 4.15EE121 pKa = 3.66LVIRR125 pKa = 11.84QDD127 pKa = 3.52EE128 pKa = 4.3NGAIPWRR135 pKa = 11.84GEE137 pKa = 3.43RR138 pKa = 11.84WSLVEE143 pKa = 3.92PEE145 pKa = 3.76IWTALTASIYY155 pKa = 10.99SVDD158 pKa = 3.6EE159 pKa = 4.28AGAPVGDD166 pKa = 4.37GEE168 pKa = 4.43

Molecular weight: 18.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653FVR4|A0A653FVR4_9CAUD Phage replication protein OS=Escherichia phage ESSI2_ev129 OX=2695848 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.67AFVTYY7 pKa = 10.54LKK9 pKa = 10.5KK10 pKa = 10.66EE11 pKa = 4.21SPAMQLPSGSTGWIEE26 pKa = 4.42LPNGQRR32 pKa = 11.84WNPGHH37 pKa = 5.86TYY39 pKa = 10.44KK40 pKa = 10.83FNAHH44 pKa = 5.92EE45 pKa = 4.08PVRR48 pKa = 11.84MKK50 pKa = 10.66SGAVLRR56 pKa = 11.84FLSTKK61 pKa = 9.73ARR63 pKa = 11.84RR64 pKa = 11.84LVGCIGGRR72 pKa = 11.84YY73 pKa = 9.41GDD75 pKa = 3.62

Molecular weight: 8.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

9716

61

679

231.3

25.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.519 ± 0.395

0.967 ± 0.138

5.496 ± 0.256

6.134 ± 0.32

3.602 ± 0.202

7.534 ± 0.339

2.007 ± 0.269

5.321 ± 0.355

5.29 ± 0.323

8.851 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.511 ± 0.167

4.23 ± 0.261

3.901 ± 0.196

4.22 ± 0.233

5.908 ± 0.48

5.949 ± 0.229

6.165 ± 0.46

6.649 ± 0.334

1.935 ± 0.172

2.81 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski