Amino acid dipepetide frequency for Aminobacter phage Erebus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.118AlaAla: 11.118 ± 1.631
0.977AlaCys: 0.977 ± 0.266
5.437AlaAsp: 5.437 ± 0.684
5.803AlaGlu: 5.803 ± 0.758
3.482AlaPhe: 3.482 ± 0.39
8.735AlaGly: 8.735 ± 0.949
1.894AlaHis: 1.894 ± 0.378
4.826AlaIle: 4.826 ± 0.638
7.147AlaLys: 7.147 ± 0.848
9.713AlaLeu: 9.713 ± 0.727
3.91AlaMet: 3.91 ± 0.528
4.215AlaAsn: 4.215 ± 0.45
3.421AlaPro: 3.421 ± 0.45
3.177AlaGln: 3.177 ± 0.452
5.62AlaArg: 5.62 ± 0.584
5.62AlaSer: 5.62 ± 0.699
6.475AlaThr: 6.475 ± 0.596
8.125AlaVal: 8.125 ± 0.81
1.833AlaTrp: 1.833 ± 0.423
2.993AlaTyr: 2.993 ± 0.428
0.0AlaXaa: 0.0 ± 0.0
Cys
1.038CysAla: 1.038 ± 0.217
0.061CysCys: 0.061 ± 0.053
0.733CysAsp: 0.733 ± 0.253
0.977CysGlu: 0.977 ± 0.267
0.244CysPhe: 0.244 ± 0.112
0.855CysGly: 0.855 ± 0.295
0.489CysHis: 0.489 ± 0.165
0.367CysIle: 0.367 ± 0.161
0.305CysLys: 0.305 ± 0.126
0.855CysLeu: 0.855 ± 0.197
0.305CysMet: 0.305 ± 0.131
0.428CysAsn: 0.428 ± 0.162
0.672CysPro: 0.672 ± 0.192
0.244CysGln: 0.244 ± 0.116
0.794CysArg: 0.794 ± 0.218
0.611CysSer: 0.611 ± 0.174
0.489CysThr: 0.489 ± 0.155
0.611CysVal: 0.611 ± 0.185
0.244CysTrp: 0.244 ± 0.12
0.183CysTyr: 0.183 ± 0.141
0.0CysXaa: 0.0 ± 0.0
Asp
5.864AspAla: 5.864 ± 0.56
0.733AspCys: 0.733 ± 0.212
3.543AspAsp: 3.543 ± 0.543
4.032AspGlu: 4.032 ± 0.544
2.321AspPhe: 2.321 ± 0.411
5.315AspGly: 5.315 ± 0.629
0.733AspHis: 0.733 ± 0.177
3.604AspIle: 3.604 ± 0.49
2.81AspLys: 2.81 ± 0.407
4.948AspLeu: 4.948 ± 0.561
1.344AspMet: 1.344 ± 0.28
2.321AspAsn: 2.321 ± 0.368
3.177AspPro: 3.177 ± 0.381
1.833AspGln: 1.833 ± 0.321
3.299AspArg: 3.299 ± 0.437
2.566AspSer: 2.566 ± 0.422
3.726AspThr: 3.726 ± 0.642
4.52AspVal: 4.52 ± 0.494
1.222AspTrp: 1.222 ± 0.288
1.161AspTyr: 1.161 ± 0.26
0.0AspXaa: 0.0 ± 0.0
Glu
7.453GluAla: 7.453 ± 1.157
0.55GluCys: 0.55 ± 0.141
2.871GluAsp: 2.871 ± 0.448
4.276GluGlu: 4.276 ± 0.712
2.382GluPhe: 2.382 ± 0.372
5.07GluGly: 5.07 ± 0.593
1.283GluHis: 1.283 ± 0.252
3.543GluIle: 3.543 ± 0.489
3.177GluLys: 3.177 ± 0.523
6.109GluLeu: 6.109 ± 0.576
1.772GluMet: 1.772 ± 0.322
2.321GluAsn: 2.321 ± 0.423
2.749GluPro: 2.749 ± 0.505
2.505GluGln: 2.505 ± 0.449
3.787GluArg: 3.787 ± 0.486
2.81GluSer: 2.81 ± 0.447
3.177GluThr: 3.177 ± 0.429
4.765GluVal: 4.765 ± 0.488
0.733GluTrp: 0.733 ± 0.195
1.955GluTyr: 1.955 ± 0.382
0.0GluXaa: 0.0 ± 0.0
Phe
4.337PheAla: 4.337 ± 0.654
0.489PheCys: 0.489 ± 0.193
2.932PheAsp: 2.932 ± 0.379
2.016PheGlu: 2.016 ± 0.341
0.977PhePhe: 0.977 ± 0.279
2.81PheGly: 2.81 ± 0.424
0.611PheHis: 0.611 ± 0.194
2.199PheIle: 2.199 ± 0.342
2.077PheLys: 2.077 ± 0.371
3.177PheLeu: 3.177 ± 0.342
0.672PheMet: 0.672 ± 0.221
1.527PheAsn: 1.527 ± 0.252
1.466PhePro: 1.466 ± 0.279
1.1PheGln: 1.1 ± 0.232
2.993PheArg: 2.993 ± 0.42
1.527PheSer: 1.527 ± 0.285
1.833PheThr: 1.833 ± 0.329
2.993PheVal: 2.993 ± 0.468
0.611PheTrp: 0.611 ± 0.171
0.672PheTyr: 0.672 ± 0.181
0.0PheXaa: 0.0 ± 0.0
Gly
6.659GlyAla: 6.659 ± 0.713
1.038GlyCys: 1.038 ± 0.241
4.887GlyAsp: 4.887 ± 0.533
5.192GlyGlu: 5.192 ± 0.679
3.849GlyPhe: 3.849 ± 0.514
5.925GlyGly: 5.925 ± 0.718
1.222GlyHis: 1.222 ± 0.274
4.52GlyIle: 4.52 ± 0.552
4.704GlyLys: 4.704 ± 0.598
6.353GlyLeu: 6.353 ± 0.61
2.627GlyMet: 2.627 ± 0.411
2.688GlyAsn: 2.688 ± 0.351
2.382GlyPro: 2.382 ± 0.379
2.566GlyGln: 2.566 ± 0.303
4.459GlyArg: 4.459 ± 0.572
3.604GlySer: 3.604 ± 0.438
4.154GlyThr: 4.154 ± 0.535
5.803GlyVal: 5.803 ± 0.617
1.588GlyTrp: 1.588 ± 0.294
2.199GlyTyr: 2.199 ± 0.339
0.0GlyXaa: 0.0 ± 0.0
His
1.833HisAla: 1.833 ± 0.309
0.611HisCys: 0.611 ± 0.215
0.794HisAsp: 0.794 ± 0.209
1.1HisGlu: 1.1 ± 0.268
0.855HisPhe: 0.855 ± 0.224
1.222HisGly: 1.222 ± 0.258
0.672HisHis: 0.672 ± 0.221
0.855HisIle: 0.855 ± 0.223
0.855HisLys: 0.855 ± 0.196
1.772HisLeu: 1.772 ± 0.371
0.733HisMet: 0.733 ± 0.233
0.733HisAsn: 0.733 ± 0.176
1.344HisPro: 1.344 ± 0.308
0.794HisGln: 0.794 ± 0.268
1.466HisArg: 1.466 ± 0.296
0.855HisSer: 0.855 ± 0.201
0.794HisThr: 0.794 ± 0.187
1.588HisVal: 1.588 ± 0.252
0.367HisTrp: 0.367 ± 0.144
0.916HisTyr: 0.916 ± 0.269
0.0HisXaa: 0.0 ± 0.0
Ile
5.009IleAla: 5.009 ± 0.582
0.794IleCys: 0.794 ± 0.211
3.421IleAsp: 3.421 ± 0.436
4.276IleGlu: 4.276 ± 0.556
1.71IlePhe: 1.71 ± 0.379
3.543IleGly: 3.543 ± 0.45
0.794IleHis: 0.794 ± 0.256
2.871IleIle: 2.871 ± 0.436
2.627IleLys: 2.627 ± 0.411
4.032IleLeu: 4.032 ± 0.511
1.283IleMet: 1.283 ± 0.276
2.688IleAsn: 2.688 ± 0.39
3.238IlePro: 3.238 ± 0.367
2.199IleGln: 2.199 ± 0.302
3.36IleArg: 3.36 ± 0.435
3.421IleSer: 3.421 ± 0.472
2.932IleThr: 2.932 ± 0.459
2.993IleVal: 2.993 ± 0.49
0.794IleTrp: 0.794 ± 0.224
1.222IleTyr: 1.222 ± 0.249
0.0IleXaa: 0.0 ± 0.0
Lys
6.353LysAla: 6.353 ± 0.862
0.489LysCys: 0.489 ± 0.165
3.177LysAsp: 3.177 ± 0.596
2.077LysGlu: 2.077 ± 0.465
2.016LysPhe: 2.016 ± 0.389
3.054LysGly: 3.054 ± 0.483
1.527LysHis: 1.527 ± 0.367
3.665LysIle: 3.665 ± 0.505
3.115LysLys: 3.115 ± 0.568
4.765LysLeu: 4.765 ± 0.482
2.199LysMet: 2.199 ± 0.411
2.016LysAsn: 2.016 ± 0.304
2.993LysPro: 2.993 ± 0.372
1.833LysGln: 1.833 ± 0.313
2.993LysArg: 2.993 ± 0.39
2.505LysSer: 2.505 ± 0.305
3.604LysThr: 3.604 ± 0.388
3.787LysVal: 3.787 ± 0.392
0.55LysTrp: 0.55 ± 0.188
1.161LysTyr: 1.161 ± 0.219
0.0LysXaa: 0.0 ± 0.0
Leu
9.591LeuAla: 9.591 ± 0.975
0.489LeuCys: 0.489 ± 0.143
6.414LeuAsp: 6.414 ± 0.596
5.376LeuGlu: 5.376 ± 0.647
2.688LeuPhe: 2.688 ± 0.391
6.659LeuGly: 6.659 ± 0.606
1.833LeuHis: 1.833 ± 0.282
3.787LeuIle: 3.787 ± 0.459
3.482LeuLys: 3.482 ± 0.431
7.208LeuLeu: 7.208 ± 0.651
2.138LeuMet: 2.138 ± 0.365
3.177LeuAsn: 3.177 ± 0.469
4.52LeuPro: 4.52 ± 0.497
2.26LeuGln: 2.26 ± 0.331
5.498LeuArg: 5.498 ± 0.522
5.864LeuSer: 5.864 ± 0.577
4.948LeuThr: 4.948 ± 0.578
5.009LeuVal: 5.009 ± 0.739
0.855LeuTrp: 0.855 ± 0.197
1.833LeuTyr: 1.833 ± 0.38
0.0LeuXaa: 0.0 ± 0.0
Met
2.932MetAla: 2.932 ± 0.403
0.305MetCys: 0.305 ± 0.114
1.405MetAsp: 1.405 ± 0.324
1.466MetGlu: 1.466 ± 0.307
0.977MetPhe: 0.977 ± 0.238
1.466MetGly: 1.466 ± 0.286
0.367MetHis: 0.367 ± 0.122
1.71MetIle: 1.71 ± 0.266
1.038MetLys: 1.038 ± 0.298
2.932MetLeu: 2.932 ± 0.413
1.038MetMet: 1.038 ± 0.228
0.916MetAsn: 0.916 ± 0.204
1.833MetPro: 1.833 ± 0.356
0.855MetGln: 0.855 ± 0.197
2.871MetArg: 2.871 ± 0.384
1.772MetSer: 1.772 ± 0.304
2.81MetThr: 2.81 ± 0.414
2.138MetVal: 2.138 ± 0.321
0.061MetTrp: 0.061 ± 0.053
0.611MetTyr: 0.611 ± 0.166
0.0MetXaa: 0.0 ± 0.0
Asn
4.704AsnAla: 4.704 ± 0.553
0.244AsnCys: 0.244 ± 0.145
2.26AsnAsp: 2.26 ± 0.466
2.077AsnGlu: 2.077 ± 0.384
1.955AsnPhe: 1.955 ± 0.334
3.726AsnGly: 3.726 ± 0.387
1.038AsnHis: 1.038 ± 0.269
1.833AsnIle: 1.833 ± 0.322
2.382AsnLys: 2.382 ± 0.419
2.871AsnLeu: 2.871 ± 0.408
1.1AsnMet: 1.1 ± 0.258
1.283AsnAsn: 1.283 ± 0.219
2.443AsnPro: 2.443 ± 0.444
1.283AsnGln: 1.283 ± 0.309
1.649AsnArg: 1.649 ± 0.355
1.527AsnSer: 1.527 ± 0.278
1.649AsnThr: 1.649 ± 0.294
2.077AsnVal: 2.077 ± 0.354
0.794AsnTrp: 0.794 ± 0.256
1.772AsnTyr: 1.772 ± 0.317
0.0AsnXaa: 0.0 ± 0.0
Pro
5.07ProAla: 5.07 ± 0.486
0.367ProCys: 0.367 ± 0.126
3.054ProAsp: 3.054 ± 0.388
3.482ProGlu: 3.482 ± 0.45
2.26ProPhe: 2.26 ± 0.375
3.726ProGly: 3.726 ± 0.424
0.611ProHis: 0.611 ± 0.202
2.138ProIle: 2.138 ± 0.413
3.054ProLys: 3.054 ± 0.541
2.932ProLeu: 2.932 ± 0.486
1.527ProMet: 1.527 ± 0.276
1.344ProAsn: 1.344 ± 0.266
2.199ProPro: 2.199 ± 0.406
1.405ProGln: 1.405 ± 0.284
2.688ProArg: 2.688 ± 0.446
2.627ProSer: 2.627 ± 0.421
4.337ProThr: 4.337 ± 0.578
3.787ProVal: 3.787 ± 0.534
0.55ProTrp: 0.55 ± 0.176
0.855ProTyr: 0.855 ± 0.224
0.0ProXaa: 0.0 ± 0.0
Gln
3.849GlnAla: 3.849 ± 0.491
0.55GlnCys: 0.55 ± 0.213
1.405GlnAsp: 1.405 ± 0.257
1.955GlnGlu: 1.955 ± 0.348
0.733GlnPhe: 0.733 ± 0.218
2.505GlnGly: 2.505 ± 0.256
0.794GlnHis: 0.794 ± 0.204
2.443GlnIle: 2.443 ± 0.302
1.527GlnLys: 1.527 ± 0.315
2.81GlnLeu: 2.81 ± 0.406
0.977GlnMet: 0.977 ± 0.21
2.077GlnAsn: 2.077 ± 0.368
1.527GlnPro: 1.527 ± 0.409
2.627GlnGln: 2.627 ± 1.212
2.321GlnArg: 2.321 ± 0.465
1.894GlnSer: 1.894 ± 0.353
2.382GlnThr: 2.382 ± 0.473
2.321GlnVal: 2.321 ± 0.442
0.855GlnTrp: 0.855 ± 0.175
0.794GlnTyr: 0.794 ± 0.2
0.0GlnXaa: 0.0 ± 0.0
Arg
4.826ArgAla: 4.826 ± 0.548
0.672ArgCys: 0.672 ± 0.204
3.421ArgAsp: 3.421 ± 0.486
3.543ArgGlu: 3.543 ± 0.525
2.26ArgPhe: 2.26 ± 0.335
3.971ArgGly: 3.971 ± 0.488
1.649ArgHis: 1.649 ± 0.35
3.421ArgIle: 3.421 ± 0.43
3.726ArgLys: 3.726 ± 0.445
5.009ArgLeu: 5.009 ± 0.536
2.077ArgMet: 2.077 ± 0.327
2.688ArgAsn: 2.688 ± 0.408
3.177ArgPro: 3.177 ± 0.382
1.955ArgGln: 1.955 ± 0.323
4.093ArgArg: 4.093 ± 0.515
3.726ArgSer: 3.726 ± 0.414
2.566ArgThr: 2.566 ± 0.534
4.704ArgVal: 4.704 ± 0.433
1.466ArgTrp: 1.466 ± 0.246
1.955ArgTyr: 1.955 ± 0.369
0.0ArgXaa: 0.0 ± 0.0
Ser
5.803SerAla: 5.803 ± 0.618
0.489SerCys: 0.489 ± 0.188
3.115SerAsp: 3.115 ± 0.42
3.543SerGlu: 3.543 ± 0.331
1.71SerPhe: 1.71 ± 0.426
4.215SerGly: 4.215 ± 0.445
0.977SerHis: 0.977 ± 0.214
2.505SerIle: 2.505 ± 0.435
2.26SerLys: 2.26 ± 0.356
3.482SerLeu: 3.482 ± 0.524
1.1SerMet: 1.1 ± 0.27
1.894SerAsn: 1.894 ± 0.288
2.566SerPro: 2.566 ± 0.41
2.443SerGln: 2.443 ± 0.38
2.443SerArg: 2.443 ± 0.449
2.443SerSer: 2.443 ± 0.396
4.032SerThr: 4.032 ± 0.395
3.971SerVal: 3.971 ± 0.554
0.611SerTrp: 0.611 ± 0.198
1.466SerTyr: 1.466 ± 0.271
0.0SerXaa: 0.0 ± 0.0
Thr
6.842ThrAla: 6.842 ± 0.686
0.794ThrCys: 0.794 ± 0.246
3.787ThrAsp: 3.787 ± 0.609
4.154ThrGlu: 4.154 ± 0.469
2.382ThrPhe: 2.382 ± 0.391
6.17ThrGly: 6.17 ± 0.665
1.283ThrHis: 1.283 ± 0.252
3.299ThrIle: 3.299 ± 0.44
2.321ThrLys: 2.321 ± 0.392
4.765ThrLeu: 4.765 ± 0.588
1.527ThrMet: 1.527 ± 0.295
1.894ThrAsn: 1.894 ± 0.312
2.81ThrPro: 2.81 ± 0.354
2.138ThrGln: 2.138 ± 0.421
3.054ThrArg: 3.054 ± 0.44
2.382ThrSer: 2.382 ± 0.346
3.482ThrThr: 3.482 ± 0.452
4.032ThrVal: 4.032 ± 0.558
1.283ThrTrp: 1.283 ± 0.258
1.894ThrTyr: 1.894 ± 0.348
0.0ThrXaa: 0.0 ± 0.0
Val
6.781ValAla: 6.781 ± 0.868
0.55ValCys: 0.55 ± 0.17
3.971ValAsp: 3.971 ± 0.497
5.315ValGlu: 5.315 ± 0.485
2.382ValPhe: 2.382 ± 0.397
4.337ValGly: 4.337 ± 0.464
1.344ValHis: 1.344 ± 0.25
4.032ValIle: 4.032 ± 0.432
4.765ValLys: 4.765 ± 0.541
6.475ValLeu: 6.475 ± 0.58
2.138ValMet: 2.138 ± 0.39
3.238ValAsn: 3.238 ± 0.374
2.993ValPro: 2.993 ± 0.446
3.115ValGln: 3.115 ± 0.689
4.093ValArg: 4.093 ± 0.542
3.421ValSer: 3.421 ± 0.511
4.52ValThr: 4.52 ± 0.571
4.459ValVal: 4.459 ± 0.54
1.1ValTrp: 1.1 ± 0.265
1.71ValTyr: 1.71 ± 0.327
0.0ValXaa: 0.0 ± 0.0
Trp
2.016TrpAla: 2.016 ± 0.352
0.0TrpCys: 0.0 ± 0.0
0.611TrpAsp: 0.611 ± 0.215
1.283TrpGlu: 1.283 ± 0.27
0.977TrpPhe: 0.977 ± 0.207
0.916TrpGly: 0.916 ± 0.256
0.428TrpHis: 0.428 ± 0.14
0.428TrpIle: 0.428 ± 0.171
0.855TrpLys: 0.855 ± 0.297
1.344TrpLeu: 1.344 ± 0.254
0.244TrpMet: 0.244 ± 0.123
0.611TrpAsn: 0.611 ± 0.192
0.733TrpPro: 0.733 ± 0.188
0.672TrpGln: 0.672 ± 0.2
1.466TrpArg: 1.466 ± 0.308
0.55TrpSer: 0.55 ± 0.191
0.855TrpThr: 0.855 ± 0.237
1.344TrpVal: 1.344 ± 0.276
0.122TrpTrp: 0.122 ± 0.085
0.489TrpTyr: 0.489 ± 0.167
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.443TyrAla: 2.443 ± 0.339
0.305TyrCys: 0.305 ± 0.134
1.833TyrAsp: 1.833 ± 0.339
1.344TyrGlu: 1.344 ± 0.302
0.855TyrPhe: 0.855 ± 0.274
2.199TyrGly: 2.199 ± 0.301
0.611TyrHis: 0.611 ± 0.179
1.161TyrIle: 1.161 ± 0.236
1.71TyrLys: 1.71 ± 0.346
2.077TyrLeu: 2.077 ± 0.312
0.733TyrMet: 0.733 ± 0.268
0.611TyrAsn: 0.611 ± 0.236
1.772TyrPro: 1.772 ± 0.418
1.344TyrGln: 1.344 ± 0.314
1.955TyrArg: 1.955 ± 0.283
1.344TyrSer: 1.344 ± 0.266
1.527TyrThr: 1.527 ± 0.295
1.71TyrVal: 1.71 ± 0.331
0.305TyrTrp: 0.305 ± 0.145
0.977TyrTyr: 0.977 ± 0.326
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 90 proteins (16371 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski