Nonlabens phage P12024S
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I6S2E7|I6S2E7_9CAUD Uncharacterized protein OS=Nonlabens phage P12024S OX=1168478 GN=P12024S_46 PE=4 SV=1
MM1 pKa = 7.22 NGYY4 pKa = 9.57 YY5 pKa = 10.9 LLLEE9 pKa = 4.43 YY10 pKa = 10.24 IKK12 pKa = 10.5 EE13 pKa = 3.99 LLISDD18 pKa = 4.22 VDD20 pKa = 4.33 CNTVTDD26 pKa = 4.12 GDD28 pKa = 4.16 GLEE31 pKa = 4.83 DD32 pKa = 5.11 ADD34 pKa = 4.55 LQRR37 pKa = 11.84 QEE39 pKa = 4.75 IYY41 pKa = 10.4 PLAHH45 pKa = 6.4 IVADD49 pKa = 4.43 DD50 pKa = 3.82 GTFVNGVMQFNLEE63 pKa = 4.23 LFALDD68 pKa = 4.79 QYY70 pKa = 11.87 DD71 pKa = 3.75 EE72 pKa = 4.24 QLDD75 pKa = 3.88 NDD77 pKa = 3.71 RR78 pKa = 11.84 DD79 pKa = 3.63 IYY81 pKa = 9.7 NTQIYY86 pKa = 8.51 VLKK89 pKa = 10.61 RR90 pKa = 11.84 VFNKK94 pKa = 10.44 LSVSEE99 pKa = 4.96 GITILGDD106 pKa = 3.32 GSFQKK111 pKa = 10.61 VEE113 pKa = 4.13 KK114 pKa = 10.58 KK115 pKa = 10.27 EE116 pKa = 3.84 NNLIGWSLSLVVEE129 pKa = 4.41 VADD132 pKa = 4.72 DD133 pKa = 3.74 VMRR136 pKa = 11.84 FCC138 pKa = 6.37
Molecular weight: 15.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.042
IPC2_protein 3.961
IPC_protein 3.935
Toseland 3.719
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.859
Rodwell 3.757
Grimsley 3.63
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.266
Thurlkill 3.77
EMBOSS 3.859
Sillero 4.05
Patrickios 3.109
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.975
Protein with the highest isoelectric point:
>tr|I6R9N7|I6R9N7_9CAUD Uncharacterized protein OS=Nonlabens phage P12024S OX=1168478 GN=P12024S_53 PE=4 SV=1
MM1 pKa = 6.88 ITQTIRR7 pKa = 11.84 TLRR10 pKa = 11.84 EE11 pKa = 3.68 TNKK14 pKa = 8.97 RR15 pKa = 11.84 TEE17 pKa = 4.06 LTEE20 pKa = 4.11 VAKK23 pKa = 10.68 GRR25 pKa = 11.84 YY26 pKa = 8.88 KK27 pKa = 10.96 YY28 pKa = 10.03 PSNWNEE34 pKa = 3.84 FKK36 pKa = 11.03 NALKK40 pKa = 10.13 WLLRR44 pKa = 11.84 KK45 pKa = 9.38 KK46 pKa = 10.55
Molecular weight: 5.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.258
IPC2_protein 9.823
IPC_protein 10.233
Toseland 10.804
ProMoST 10.423
Dawson 10.877
Bjellqvist 10.496
Wikipedia 11.023
Rodwell 11.374
Grimsley 10.906
Solomon 10.95
Lehninger 10.935
Nozaki 10.76
DTASelect 10.496
Thurlkill 10.774
EMBOSS 11.184
Sillero 10.789
Patrickios 11.14
IPC_peptide 10.95
IPC2_peptide 9.048
IPC2.peptide.svr19 8.683
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
59
0
59
11087
38
754
187.9
21.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.278 ± 0.518
0.956 ± 0.134
6.864 ± 0.252
7.153 ± 0.354
4.636 ± 0.245
6.178 ± 0.272
1.335 ± 0.188
7.324 ± 0.259
8.487 ± 0.515
8.74 ± 0.283
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.849 ± 0.193
6.07 ± 0.319
3.022 ± 0.236
3.662 ± 0.217
4.014 ± 0.195
6.233 ± 0.342
6.16 ± 0.376
5.628 ± 0.256
1.299 ± 0.158
4.113 ± 0.329
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here