Aedes aegypti (Yellowfever mosquito) (Culex aegypti)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Nematocera; Culicomorpha; Culicoidea<

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21531 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q16J97|Q16J97_AEDAE Isoform of A0A6I8TFF7 AAEL013402-PB (Fragment) OS=Aedes aegypti OX=7159 GN=AAEL013402 PE=4 SV=1
MM1 pKa = 7.46NSCEE5 pKa = 4.5LKK7 pKa = 9.93TIGWNTKK14 pKa = 8.61KK15 pKa = 10.45RR16 pKa = 11.84CSSEE20 pKa = 3.97VAEE23 pKa = 5.39LIAPSIPDD31 pKa = 3.36VASFSYY37 pKa = 8.93ITLAKK42 pKa = 9.89EE43 pKa = 3.97SPNRR47 pKa = 11.84VLEE50 pKa = 4.22SDD52 pKa = 3.4EE53 pKa = 5.47DD54 pKa = 3.92NYY56 pKa = 11.59NSFNEE61 pKa = 4.06QEE63 pKa = 3.71TDD65 pKa = 3.01ICHH68 pKa = 6.85DD69 pKa = 3.85SSDD72 pKa = 3.99ISDD75 pKa = 4.1SDD77 pKa = 3.64TDD79 pKa = 4.13SQCSSISQPSLDD91 pKa = 4.5DD92 pKa = 3.77GRR94 pKa = 11.84VDD96 pKa = 4.24TIATMEE102 pKa = 4.08VVEE105 pKa = 4.58YY106 pKa = 9.64EE107 pKa = 4.33VASATEE113 pKa = 4.16SEE115 pKa = 4.68DD116 pKa = 4.68DD117 pKa = 3.75YY118 pKa = 11.98SSDD121 pKa = 3.3VSSGTEE127 pKa = 3.36VRR129 pKa = 11.84LYY131 pKa = 10.73VGSICTSGCRR141 pKa = 3.69

Molecular weight: 15.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I8TQK0|A0A6I8TQK0_AEDAE Spectrin OS=Aedes aegypti OX=7159 GN=5566099 PE=3 SV=1
SS1 pKa = 6.85SSWKK5 pKa = 9.92RR6 pKa = 11.84SSSSSWKK13 pKa = 9.94RR14 pKa = 11.84SSSSSWKK21 pKa = 9.94RR22 pKa = 11.84SSSSSWKK29 pKa = 9.94RR30 pKa = 11.84SSSSSWKK37 pKa = 9.94RR38 pKa = 11.84SSSSSWKK45 pKa = 9.94RR46 pKa = 11.84SSSSSWKK53 pKa = 9.94RR54 pKa = 11.84SSSSSWKK61 pKa = 9.94RR62 pKa = 11.84SSSSSWKK69 pKa = 9.94RR70 pKa = 11.84SSSSSWKK77 pKa = 9.94RR78 pKa = 11.84SSSSSWKK85 pKa = 9.94RR86 pKa = 11.84SSSSSWKK93 pKa = 9.83RR94 pKa = 11.84SSFSFWNRR102 pKa = 11.84SSFSSWKK109 pKa = 9.92RR110 pKa = 11.84SSSSSWKK117 pKa = 10.08RR118 pKa = 11.84SFSSFWKK125 pKa = 10.08RR126 pKa = 11.84SSFSFWKK133 pKa = 10.22RR134 pKa = 11.84SSSSSWKK141 pKa = 9.94RR142 pKa = 11.84SSSSSWKK149 pKa = 9.94RR150 pKa = 11.84SSSSSWKK157 pKa = 9.94RR158 pKa = 11.84SSSSSWKK165 pKa = 9.94RR166 pKa = 11.84SSSSSWKK173 pKa = 9.98RR174 pKa = 11.84SSSS177 pKa = 3.26

Molecular weight: 20.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14563

6968

21531

14460630

31

16267

671.6

75.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.549 ± 0.014

1.795 ± 0.018

5.519 ± 0.013

6.572 ± 0.019

3.532 ± 0.013

5.887 ± 0.017

2.629 ± 0.011

5.285 ± 0.012

5.995 ± 0.018

8.577 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.313 ± 0.007

5.031 ± 0.014

5.527 ± 0.024

4.835 ± 0.023

5.321 ± 0.012

8.812 ± 0.027

5.95 ± 0.019

5.989 ± 0.012

0.943 ± 0.005

2.94 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski