Colwellia sp. PAMC 20917

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Colwelliaceae; Colwellia; unclassified Colwellia

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3764 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8RVC5|A0A1D8RVC5_9GAMM Uncharacterized protein OS=Colwellia sp. PAMC 20917 OX=1816218 GN=A3Q34_09475 PE=3 SV=1
MM1 pKa = 7.33TNKK4 pKa = 9.65FNKK7 pKa = 9.41IVSALVLCGATTFASASDD25 pKa = 3.73MQIDD29 pKa = 3.87PTATPGGTLTSIFSSITADD48 pKa = 3.56EE49 pKa = 4.43IKK51 pKa = 10.43PVSAYY56 pKa = 10.58FDD58 pKa = 4.3LDD60 pKa = 3.74NNGITTGDD68 pKa = 3.87LVFDD72 pKa = 4.12SAFGEE77 pKa = 4.41QFGSLNPTNSDD88 pKa = 3.0LGFNVAPAGWTLFADD103 pKa = 3.84YY104 pKa = 10.09EE105 pKa = 4.6FYY107 pKa = 11.12GAAIVIDD114 pKa = 4.19GLLDD118 pKa = 3.61SDD120 pKa = 4.2GNGYY124 pKa = 10.57SDD126 pKa = 5.69ADD128 pKa = 3.73LDD130 pKa = 4.35EE131 pKa = 5.48NGVIGNILYY140 pKa = 10.78GSTTDD145 pKa = 4.16LDD147 pKa = 3.66LWQNQPGNTGNADD160 pKa = 3.41GVLDD164 pKa = 3.81TGEE167 pKa = 3.92EE168 pKa = 3.79LAAQFTGGYY177 pKa = 9.78INVFLNTPYY186 pKa = 9.94TASEE190 pKa = 4.05SSGAFDD196 pKa = 4.8GYY198 pKa = 11.35NGIGARR204 pKa = 11.84TLDD207 pKa = 4.1ANTQLAMRR215 pKa = 11.84FNVTGSSLVDD225 pKa = 3.2VSIQIYY231 pKa = 9.36SQLSMIIDD239 pKa = 4.11DD240 pKa = 4.58FLYY243 pKa = 10.73SPGVGDD249 pKa = 3.65VKK251 pKa = 11.1DD252 pKa = 4.3YY253 pKa = 11.34IDD255 pKa = 4.18LAGNNAFGTFSSEE268 pKa = 4.57LNGLSNSPTVNTDD281 pKa = 3.16YY282 pKa = 11.27VISDD286 pKa = 3.91LQNGVINNILNTSVAYY302 pKa = 10.34NAGTNGNVSVKK313 pKa = 9.7TRR315 pKa = 11.84TTTIASANLLVSVPEE330 pKa = 4.51PGTIALFGLALIGFAGSRR348 pKa = 11.84KK349 pKa = 9.53RR350 pKa = 11.84KK351 pKa = 9.7SSS353 pKa = 3.14

Molecular weight: 36.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8RW29|A0A1D8RW29_9GAMM Transposase OS=Colwellia sp. PAMC 20917 OX=1816218 GN=A3Q34_02430 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.21NGRR28 pKa = 11.84AVISRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3764

0

3764

1268406

44

5451

337.0

37.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.084 ± 0.04

0.991 ± 0.014

5.66 ± 0.055

5.933 ± 0.041

4.383 ± 0.029

6.292 ± 0.044

2.12 ± 0.021

7.315 ± 0.038

6.141 ± 0.049

10.32 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.345 ± 0.023

5.202 ± 0.042

3.458 ± 0.022

4.452 ± 0.041

3.824 ± 0.03

7.016 ± 0.038

5.682 ± 0.068

6.565 ± 0.037

1.095 ± 0.016

3.12 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski