Amino acid dipepetide frequency for Candidatus Gracilibacteria bacterium GN02-873

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.167AlaAla: 3.167 ± 0.154
0.499AlaCys: 0.499 ± 0.043
3.475AlaAsp: 3.475 ± 0.112
4.894AlaGlu: 4.894 ± 0.135
3.03AlaPhe: 3.03 ± 0.112
4.344AlaGly: 4.344 ± 0.148
1.275AlaHis: 1.275 ± 0.06
5.518AlaIle: 5.518 ± 0.178
5.54AlaLys: 5.54 ± 0.125
5.569AlaLeu: 5.569 ± 0.165
1.831AlaMet: 1.831 ± 0.079
3.174AlaAsn: 3.174 ± 0.135
1.885AlaPro: 1.885 ± 0.078
2.653AlaGln: 2.653 ± 0.129
2.987AlaArg: 2.987 ± 0.127
3.896AlaSer: 3.896 ± 0.148
4.068AlaThr: 4.068 ± 0.134
3.199AlaVal: 3.199 ± 0.138
0.495AlaTrp: 0.495 ± 0.04
2.054AlaTyr: 2.054 ± 0.089
0.0AlaXaa: 0.0 ± 0.0
Cys
0.434CysAla: 0.434 ± 0.044
0.065CysCys: 0.065 ± 0.015
0.434CysAsp: 0.434 ± 0.037
0.596CysGlu: 0.596 ± 0.055
0.355CysPhe: 0.355 ± 0.038
0.65CysGly: 0.65 ± 0.055
0.223CysHis: 0.223 ± 0.031
0.528CysIle: 0.528 ± 0.046
0.424CysLys: 0.424 ± 0.039
0.506CysLeu: 0.506 ± 0.045
0.169CysMet: 0.169 ± 0.026
0.312CysAsn: 0.312 ± 0.041
0.381CysPro: 0.381 ± 0.054
0.341CysGln: 0.341 ± 0.04
0.302CysArg: 0.302 ± 0.031
0.456CysSer: 0.456 ± 0.037
0.503CysThr: 0.503 ± 0.044
0.478CysVal: 0.478 ± 0.044
0.065CysTrp: 0.065 ± 0.015
0.269CysTyr: 0.269 ± 0.03
0.0CysXaa: 0.0 ± 0.0
Asp
4.194AspAla: 4.194 ± 0.122
0.33AspCys: 0.33 ± 0.034
3.009AspAsp: 3.009 ± 0.118
4.707AspGlu: 4.707 ± 0.161
4.107AspPhe: 4.107 ± 0.15
3.54AspGly: 3.54 ± 0.134
0.632AspHis: 0.632 ± 0.042
4.592AspIle: 4.592 ± 0.15
3.091AspLys: 3.091 ± 0.141
4.341AspLeu: 4.341 ± 0.127
1.224AspMet: 1.224 ± 0.066
2.298AspAsn: 2.298 ± 0.105
1.655AspPro: 1.655 ± 0.087
1.271AspGln: 1.271 ± 0.083
1.845AspArg: 1.845 ± 0.085
2.966AspSer: 2.966 ± 0.118
2.858AspThr: 2.858 ± 0.104
2.836AspVal: 2.836 ± 0.108
0.625AspTrp: 0.625 ± 0.051
1.885AspTyr: 1.885 ± 0.089
0.0AspXaa: 0.0 ± 0.0
Glu
4.125GluAla: 4.125 ± 0.157
0.481GluCys: 0.481 ± 0.051
3.235GluAsp: 3.235 ± 0.098
5.07GluGlu: 5.07 ± 0.247
3.253GluPhe: 3.253 ± 0.121
3.364GluGly: 3.364 ± 0.116
1.099GluHis: 1.099 ± 0.065
7.529GluIle: 7.529 ± 0.194
10.577GluLys: 10.577 ± 0.288
5.623GluLeu: 5.623 ± 0.171
1.885GluMet: 1.885 ± 0.08
7.558GluAsn: 7.558 ± 0.215
1.433GluPro: 1.433 ± 0.068
2.488GluGln: 2.488 ± 0.112
2.897GluArg: 2.897 ± 0.115
3.429GluSer: 3.429 ± 0.107
3.838GluThr: 3.838 ± 0.135
3.44GluVal: 3.44 ± 0.147
0.635GluTrp: 0.635 ± 0.053
2.79GluTyr: 2.79 ± 0.117
0.0GluXaa: 0.0 ± 0.0
Phe
3.791PheAla: 3.791 ± 0.134
0.585PheCys: 0.585 ± 0.045
3.023PheAsp: 3.023 ± 0.114
4.122PheGlu: 4.122 ± 0.142
4.535PhePhe: 4.535 ± 0.188
4.255PheGly: 4.255 ± 0.139
1.016PheHis: 1.016 ± 0.071
3.601PheIle: 3.601 ± 0.14
2.37PheLys: 2.37 ± 0.101
6.886PheLeu: 6.886 ± 0.218
1.117PheMet: 1.117 ± 0.072
1.731PheAsn: 1.731 ± 0.076
2.33PhePro: 2.33 ± 0.085
1.975PheGln: 1.975 ± 0.086
2.133PheArg: 2.133 ± 0.089
5.633PheSer: 5.633 ± 0.209
2.61PheThr: 2.61 ± 0.101
3.605PheVal: 3.605 ± 0.128
0.75PheTrp: 0.75 ± 0.056
2.086PheTyr: 2.086 ± 0.101
0.0PheXaa: 0.0 ± 0.0
Gly
4.014GlyAla: 4.014 ± 0.137
0.442GlyCys: 0.442 ± 0.056
3.228GlyAsp: 3.228 ± 0.121
4.434GlyGlu: 4.434 ± 0.147
3.45GlyPhe: 3.45 ± 0.114
4.0GlyGly: 4.0 ± 0.17
1.102GlyHis: 1.102 ± 0.067
5.536GlyIle: 5.536 ± 0.193
6.096GlyLys: 6.096 ± 0.166
4.797GlyLeu: 4.797 ± 0.163
1.486GlyMet: 1.486 ± 0.067
3.619GlyAsn: 3.619 ± 0.151
1.135GlyPro: 1.135 ± 0.065
2.025GlyGln: 2.025 ± 0.09
2.467GlyArg: 2.467 ± 0.103
3.526GlySer: 3.526 ± 0.14
3.565GlyThr: 3.565 ± 0.143
4.19GlyVal: 4.19 ± 0.176
0.528GlyTrp: 0.528 ± 0.045
2.441GlyTyr: 2.441 ± 0.098
0.0GlyXaa: 0.0 ± 0.0
His
1.081HisAla: 1.081 ± 0.054
0.194HisCys: 0.194 ± 0.029
0.984HisAsp: 0.984 ± 0.06
1.425HisGlu: 1.425 ± 0.074
1.21HisPhe: 1.21 ± 0.077
1.156HisGly: 1.156 ± 0.079
0.513HisHis: 0.513 ± 0.047
1.673HisIle: 1.673 ± 0.077
1.156HisLys: 1.156 ± 0.068
1.404HisLeu: 1.404 ± 0.069
0.373HisMet: 0.373 ± 0.032
0.865HisAsn: 0.865 ± 0.057
0.797HisPro: 0.797 ± 0.06
0.736HisGln: 0.736 ± 0.043
0.711HisArg: 0.711 ± 0.054
1.109HisSer: 1.109 ± 0.054
1.12HisThr: 1.12 ± 0.066
0.574HisVal: 0.574 ± 0.045
0.147HisTrp: 0.147 ± 0.025
0.635HisTyr: 0.635 ± 0.046
0.0HisXaa: 0.0 ± 0.0
Ile
6.071IleAla: 6.071 ± 0.153
0.707IleCys: 0.707 ± 0.057
4.779IleAsp: 4.779 ± 0.13
6.201IleGlu: 6.201 ± 0.174
7.518IlePhe: 7.518 ± 0.281
5.307IleGly: 5.307 ± 0.131
1.569IleHis: 1.569 ± 0.074
7.192IleIle: 7.192 ± 0.238
4.825IleLys: 4.825 ± 0.153
9.486IleLeu: 9.486 ± 0.259
1.576IleMet: 1.576 ± 0.091
3.181IleAsn: 3.181 ± 0.107
3.396IlePro: 3.396 ± 0.12
2.912IleGln: 2.912 ± 0.101
3.501IleArg: 3.501 ± 0.1
7.105IleSer: 7.105 ± 0.201
4.65IleThr: 4.65 ± 0.169
4.994IleVal: 4.994 ± 0.147
0.607IleTrp: 0.607 ± 0.051
2.517IleTyr: 2.517 ± 0.101
0.0IleXaa: 0.0 ± 0.0
Lys
3.659LysAla: 3.659 ± 0.141
0.463LysCys: 0.463 ± 0.044
3.231LysAsp: 3.231 ± 0.13
4.689LysGlu: 4.689 ± 0.156
4.154LysPhe: 4.154 ± 0.142
3.386LysGly: 3.386 ± 0.108
1.012LysHis: 1.012 ± 0.058
10.426LysIle: 10.426 ± 0.249
8.682LysLys: 8.682 ± 0.235
5.332LysLeu: 5.332 ± 0.158
2.962LysMet: 2.962 ± 0.11
8.983LysAsn: 8.983 ± 0.207
2.154LysPro: 2.154 ± 0.099
2.172LysGln: 2.172 ± 0.096
2.366LysArg: 2.366 ± 0.097
4.359LysSer: 4.359 ± 0.133
4.886LysThr: 4.886 ± 0.156
3.389LysVal: 3.389 ± 0.124
0.592LysTrp: 0.592 ± 0.041
3.321LysTyr: 3.321 ± 0.112
0.0LysXaa: 0.0 ± 0.0
Leu
6.445LeuAla: 6.445 ± 0.166
0.614LeuCys: 0.614 ± 0.049
4.89LeuAsp: 4.89 ± 0.13
7.479LeuGlu: 7.479 ± 0.191
4.204LeuPhe: 4.204 ± 0.147
5.508LeuGly: 5.508 ± 0.156
1.662LeuHis: 1.662 ± 0.086
5.892LeuIle: 5.892 ± 0.167
6.258LeuLys: 6.258 ± 0.173
7.339LeuLeu: 7.339 ± 0.211
2.032LeuMet: 2.032 ± 0.087
3.896LeuAsn: 3.896 ± 0.135
3.414LeuPro: 3.414 ± 0.141
3.533LeuGln: 3.533 ± 0.119
3.608LeuArg: 3.608 ± 0.114
6.603LeuSer: 6.603 ± 0.187
4.484LeuThr: 4.484 ± 0.137
4.955LeuVal: 4.955 ± 0.156
0.679LeuTrp: 0.679 ± 0.052
2.883LeuTyr: 2.883 ± 0.121
0.0LeuXaa: 0.0 ± 0.0
Met
1.598MetAla: 1.598 ± 0.082
0.154MetCys: 0.154 ± 0.026
1.056MetAsp: 1.056 ± 0.062
1.433MetGlu: 1.433 ± 0.082
0.966MetPhe: 0.966 ± 0.064
1.447MetGly: 1.447 ± 0.076
0.463MetHis: 0.463 ± 0.048
2.179MetIle: 2.179 ± 0.098
2.111MetLys: 2.111 ± 0.079
2.093MetLeu: 2.093 ± 0.085
0.722MetMet: 0.722 ± 0.053
1.429MetAsn: 1.429 ± 0.075
0.801MetPro: 0.801 ± 0.053
1.124MetGln: 1.124 ± 0.064
1.267MetArg: 1.267 ± 0.068
1.565MetSer: 1.565 ± 0.07
1.397MetThr: 1.397 ± 0.069
1.12MetVal: 1.12 ± 0.06
0.151MetTrp: 0.151 ± 0.023
0.722MetTyr: 0.722 ± 0.056
0.0MetXaa: 0.0 ± 0.0
Asn
3.838AsnAla: 3.838 ± 0.148
0.449AsnCys: 0.449 ± 0.04
2.42AsnAsp: 2.42 ± 0.1
3.63AsnGlu: 3.63 ± 0.121
4.581AsnPhe: 4.581 ± 0.148
3.472AsnGly: 3.472 ± 0.16
1.023AsnHis: 1.023 ± 0.062
6.348AsnIle: 6.348 ± 0.173
3.098AsnLys: 3.098 ± 0.12
5.68AsnLeu: 5.68 ± 0.148
1.048AsnMet: 1.048 ± 0.066
3.425AsnAsn: 3.425 ± 0.147
3.059AsnPro: 3.059 ± 0.127
1.914AsnGln: 1.914 ± 0.09
2.187AsnArg: 2.187 ± 0.086
4.344AsnSer: 4.344 ± 0.143
3.303AsnThr: 3.303 ± 0.138
2.718AsnVal: 2.718 ± 0.122
0.51AsnTrp: 0.51 ± 0.042
2.011AsnTyr: 2.011 ± 0.089
0.0AsnXaa: 0.0 ± 0.0
Pro
1.914ProAla: 1.914 ± 0.101
0.176ProCys: 0.176 ± 0.026
1.827ProAsp: 1.827 ± 0.097
3.368ProGlu: 3.368 ± 0.12
1.788ProPhe: 1.788 ± 0.085
2.021ProGly: 2.021 ± 0.101
0.747ProHis: 0.747 ± 0.053
2.66ProIle: 2.66 ± 0.094
2.966ProLys: 2.966 ± 0.111
2.416ProLeu: 2.416 ± 0.102
0.851ProMet: 0.851 ± 0.059
2.057ProAsn: 2.057 ± 0.079
1.005ProPro: 1.005 ± 0.079
1.303ProGln: 1.303 ± 0.1
1.228ProArg: 1.228 ± 0.072
1.975ProSer: 1.975 ± 0.092
2.054ProThr: 2.054 ± 0.122
1.813ProVal: 1.813 ± 0.082
0.262ProTrp: 0.262 ± 0.033
1.149ProTyr: 1.149 ± 0.063
0.0ProXaa: 0.0 ± 0.0
Gln
2.108GlnAla: 2.108 ± 0.092
0.226GlnCys: 0.226 ± 0.032
1.652GlnAsp: 1.652 ± 0.081
2.844GlnGlu: 2.844 ± 0.116
1.569GlnPhe: 1.569 ± 0.081
1.813GlnGly: 1.813 ± 0.089
0.438GlnHis: 0.438 ± 0.042
3.192GlnIle: 3.192 ± 0.114
4.89GlnLys: 4.89 ± 0.147
2.201GlnLeu: 2.201 ± 0.105
0.869GlnMet: 0.869 ± 0.053
3.371GlnAsn: 3.371 ± 0.128
0.905GlnPro: 0.905 ± 0.11
1.228GlnGln: 1.228 ± 0.098
1.077GlnArg: 1.077 ± 0.063
1.928GlnSer: 1.928 ± 0.089
2.05GlnThr: 2.05 ± 0.098
1.727GlnVal: 1.727 ± 0.092
0.226GlnTrp: 0.226 ± 0.034
1.253GlnTyr: 1.253 ± 0.072
0.0GlnXaa: 0.0 ± 0.0
Arg
2.89ArgAla: 2.89 ± 0.126
0.251ArgCys: 0.251 ± 0.029
2.388ArgAsp: 2.388 ± 0.095
3.544ArgGlu: 3.544 ± 0.135
1.982ArgPhe: 1.982 ± 0.088
2.485ArgGly: 2.485 ± 0.089
0.7ArgHis: 0.7 ± 0.048
3.217ArgIle: 3.217 ± 0.113
3.734ArgLys: 3.734 ± 0.108
3.149ArgLeu: 3.149 ± 0.111
0.977ArgMet: 0.977 ± 0.065
2.158ArgAsn: 2.158 ± 0.102
1.21ArgPro: 1.21 ± 0.076
1.354ArgGln: 1.354 ± 0.085
1.608ArgArg: 1.608 ± 0.074
1.957ArgSer: 1.957 ± 0.091
2.064ArgThr: 2.064 ± 0.076
2.528ArgVal: 2.528 ± 0.113
0.287ArgTrp: 0.287 ± 0.034
1.515ArgTyr: 1.515 ± 0.072
0.0ArgXaa: 0.0 ± 0.0
Ser
4.219SerAla: 4.219 ± 0.143
0.596SerCys: 0.596 ± 0.053
3.77SerAsp: 3.77 ± 0.144
5.325SerGlu: 5.325 ± 0.174
3.228SerPhe: 3.228 ± 0.125
4.897SerGly: 4.897 ± 0.155
1.393SerHis: 1.393 ± 0.072
4.459SerIle: 4.459 ± 0.144
4.025SerLys: 4.025 ± 0.135
6.175SerLeu: 6.175 ± 0.145
1.3SerMet: 1.3 ± 0.065
2.768SerAsn: 2.768 ± 0.153
2.233SerPro: 2.233 ± 0.091
2.797SerGln: 2.797 ± 0.105
3.045SerArg: 3.045 ± 0.11
4.043SerSer: 4.043 ± 0.2
3.221SerThr: 3.221 ± 0.143
4.14SerVal: 4.14 ± 0.135
0.589SerTrp: 0.589 ± 0.055
2.115SerTyr: 2.115 ± 0.096
0.0SerXaa: 0.0 ± 0.0
Thr
3.443ThrAla: 3.443 ± 0.134
0.37ThrCys: 0.37 ± 0.054
3.059ThrAsp: 3.059 ± 0.109
4.237ThrGlu: 4.237 ± 0.15
2.736ThrPhe: 2.736 ± 0.1
3.874ThrGly: 3.874 ± 0.138
1.156ThrHis: 1.156 ± 0.061
5.339ThrIle: 5.339 ± 0.15
3.935ThrLys: 3.935 ± 0.118
5.127ThrLeu: 5.127 ± 0.149
1.142ThrMet: 1.142 ± 0.067
3.095ThrAsn: 3.095 ± 0.14
2.635ThrPro: 2.635 ± 0.124
2.072ThrGln: 2.072 ± 0.105
2.187ThrArg: 2.187 ± 0.086
3.109ThrSer: 3.109 ± 0.104
3.763ThrThr: 3.763 ± 0.158
2.675ThrVal: 2.675 ± 0.114
0.377ThrTrp: 0.377 ± 0.035
1.67ThrTyr: 1.67 ± 0.078
0.0ThrXaa: 0.0 ± 0.0
Val
4.093ValAla: 4.093 ± 0.144
0.46ValCys: 0.46 ± 0.041
3.095ValAsp: 3.095 ± 0.109
3.82ValGlu: 3.82 ± 0.136
2.783ValPhe: 2.783 ± 0.104
3.888ValGly: 3.888 ± 0.155
0.959ValHis: 0.959 ± 0.066
4.409ValIle: 4.409 ± 0.137
3.289ValLys: 3.289 ± 0.106
4.481ValLeu: 4.481 ± 0.148
1.393ValMet: 1.393 ± 0.075
2.456ValAsn: 2.456 ± 0.115
1.942ValPro: 1.942 ± 0.095
1.881ValGln: 1.881 ± 0.08
2.528ValArg: 2.528 ± 0.104
3.562ValSer: 3.562 ± 0.111
3.224ValThr: 3.224 ± 0.13
3.691ValVal: 3.691 ± 0.135
0.399ValTrp: 0.399 ± 0.035
1.684ValTyr: 1.684 ± 0.087
0.0ValXaa: 0.0 ± 0.0
Trp
0.427TrpAla: 0.427 ± 0.035
0.068TrpCys: 0.068 ± 0.015
0.546TrpAsp: 0.546 ± 0.046
0.567TrpGlu: 0.567 ± 0.05
0.33TrpPhe: 0.33 ± 0.034
0.481TrpGly: 0.481 ± 0.037
0.162TrpHis: 0.162 ± 0.024
0.786TrpIle: 0.786 ± 0.062
0.837TrpLys: 0.837 ± 0.061
0.797TrpLeu: 0.797 ± 0.057
0.201TrpMet: 0.201 ± 0.024
0.596TrpAsn: 0.596 ± 0.05
0.172TrpPro: 0.172 ± 0.028
0.359TrpGln: 0.359 ± 0.038
0.406TrpArg: 0.406 ± 0.036
0.463TrpSer: 0.463 ± 0.045
0.402TrpThr: 0.402 ± 0.035
0.366TrpVal: 0.366 ± 0.037
0.14TrpTrp: 0.14 ± 0.026
0.305TrpTyr: 0.305 ± 0.035
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.043TyrAla: 2.043 ± 0.091
0.359TyrCys: 0.359 ± 0.041
1.906TyrAsp: 1.906 ± 0.077
2.366TyrGlu: 2.366 ± 0.094
2.373TyrPhe: 2.373 ± 0.11
2.194TyrGly: 2.194 ± 0.099
0.725TyrHis: 0.725 ± 0.048
3.016TyrIle: 3.016 ± 0.112
2.316TyrLys: 2.316 ± 0.095
2.976TyrLeu: 2.976 ± 0.118
0.635TyrMet: 0.635 ± 0.047
2.086TyrAsn: 2.086 ± 0.091
1.038TyrPro: 1.038 ± 0.062
1.429TyrGln: 1.429 ± 0.072
1.648TyrArg: 1.648 ± 0.09
2.309TyrSer: 2.309 ± 0.099
1.939TyrThr: 1.939 ± 0.086
1.598TyrVal: 1.598 ± 0.075
0.348TyrTrp: 0.348 ± 0.037
1.328TyrTyr: 1.328 ± 0.075
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 908 proteins (278524 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski