Streptococcus satellite phage Javan593
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZTQ6|A0A4D5ZTQ6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan593 OX=2558763 GN=JavanS593_0016 PE=4 SV=1
MM1 pKa = 7.56 TEE3 pKa = 3.71 QEE5 pKa = 4.16 YY6 pKa = 9.56 FAQAEE11 pKa = 4.43 KK12 pKa = 10.67 EE13 pKa = 4.12 LEE15 pKa = 3.97 EE16 pKa = 5.29 LNQQRR21 pKa = 11.84 ADD23 pKa = 3.91 FMAMDD28 pKa = 4.34 FEE30 pKa = 4.67 EE31 pKa = 5.98 LNTADD36 pKa = 4.21 YY37 pKa = 11.27 INFLEE42 pKa = 4.21 IGNRR46 pKa = 11.84 IVAEE50 pKa = 4.1 DD51 pKa = 3.43 VTLNVYY57 pKa = 9.26 EE58 pKa = 4.74 LYY60 pKa = 10.55 KK61 pKa = 11.05 HH62 pKa = 6.84 PDD64 pKa = 2.79 TRR66 pKa = 11.84 AKK68 pKa = 10.46 FFATIAKK75 pKa = 9.47 IAYY78 pKa = 8.24 HH79 pKa = 5.81 VNNMFQTADD88 pKa = 3.41 RR89 pKa = 11.84 MEE91 pKa = 4.33 AMIDD95 pKa = 3.46 SLEE98 pKa = 3.8 QHH100 pKa = 5.84 YY101 pKa = 11.25 QNTIKK106 pKa = 10.85 KK107 pKa = 9.71 LVYY110 pKa = 9.26 QTDD113 pKa = 3.56 SDD115 pKa = 4.12 KK116 pKa = 11.29 LAEE119 pKa = 4.57 LLLEE123 pKa = 4.38 VKK125 pKa = 10.18 KK126 pKa = 10.9 DD127 pKa = 3.87 NPNMTAEE134 pKa = 4.19 QEE136 pKa = 4.42 SQFIRR141 pKa = 11.84 DD142 pKa = 3.67 VAVSGLLAKK151 pKa = 10.56 EE152 pKa = 4.02 NN153 pKa = 3.7
Molecular weight: 17.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.554
IPC2_protein 4.52
IPC_protein 4.431
Toseland 4.279
ProMoST 4.533
Dawson 4.368
Bjellqvist 4.52
Wikipedia 4.228
Rodwell 4.279
Grimsley 4.19
Solomon 4.368
Lehninger 4.329
Nozaki 4.482
DTASelect 4.609
Thurlkill 4.279
EMBOSS 4.253
Sillero 4.546
Patrickios 3.834
IPC_peptide 4.38
IPC2_peptide 4.533
IPC2.peptide.svr19 4.485
Protein with the highest isoelectric point:
>tr|A0A4D5ZWD1|A0A4D5ZWD1_9VIRU Integrase OS=Streptococcus satellite phage Javan593 OX=2558763 GN=JavanS593_0017 PE=3 SV=1
MM1 pKa = 7.65 KK2 pKa = 10.14 IKK4 pKa = 10.43 EE5 pKa = 4.14 VTKK8 pKa = 10.29 KK9 pKa = 10.58 DD10 pKa = 3.16 GSIVYY15 pKa = 10.15 RR16 pKa = 11.84 SNIYY20 pKa = 10.61 LGVDD24 pKa = 3.26 QITGKK29 pKa = 10.2 KK30 pKa = 9.93 VKK32 pKa = 9.98 TNVTGRR38 pKa = 11.84 TQKK41 pKa = 10.12 EE42 pKa = 3.98 VRR44 pKa = 11.84 QKK46 pKa = 11.28 AKK48 pKa = 9.23 QAEE51 pKa = 4.54 YY52 pKa = 10.95 NFKK55 pKa = 10.73 QSGSTRR61 pKa = 11.84 FCAQTDD67 pKa = 2.98 IKK69 pKa = 10.02 TIEE72 pKa = 4.23 EE73 pKa = 4.36 LSEE76 pKa = 3.9 SWLANYY82 pKa = 9.94 QNTVKK87 pKa = 10.37 PQTLRR92 pKa = 11.84 NTKK95 pKa = 10.24 SYY97 pKa = 10.52 LKK99 pKa = 10.53 NHH101 pKa = 6.59 ILPRR105 pKa = 11.84 LGDD108 pKa = 3.47 MRR110 pKa = 11.84 LEE112 pKa = 4.2 KK113 pKa = 10.58 LSPPVAQAFINDD125 pKa = 3.68 LAKK128 pKa = 9.9 RR129 pKa = 11.84 TNQFDD134 pKa = 3.59 KK135 pKa = 11.02 ARR137 pKa = 11.84 SVLSRR142 pKa = 11.84 MFRR145 pKa = 11.84 YY146 pKa = 10.18 AIVLQLVQYY155 pKa = 10.86 NPVRR159 pKa = 11.84 DD160 pKa = 3.64 TLVPRR165 pKa = 11.84 KK166 pKa = 9.55 KK167 pKa = 10.18 QSAKK171 pKa = 10.62 KK172 pKa = 8.61 KK173 pKa = 9.34 VKK175 pKa = 10.38 YY176 pKa = 9.74 IQSDD180 pKa = 3.63 HH181 pKa = 6.4 LKK183 pKa = 10.68 KK184 pKa = 10.75 FLDD187 pKa = 5.06 HH188 pKa = 7.05 IDD190 pKa = 3.63 KK191 pKa = 10.65 LAQKK195 pKa = 10.73 DD196 pKa = 3.84 FTRR199 pKa = 11.84 YY200 pKa = 9.52 GYY202 pKa = 9.19 MVAFRR207 pKa = 11.84 LLLATGMRR215 pKa = 11.84 IGEE218 pKa = 4.32 LSALEE223 pKa = 3.83 WSDD226 pKa = 3.25 INLEE230 pKa = 4.02 EE231 pKa = 4.42 HH232 pKa = 7.15 SIRR235 pKa = 11.84 INKK238 pKa = 8.86 TYY240 pKa = 10.28 LQEE243 pKa = 4.02 IKK245 pKa = 10.5 QVGEE249 pKa = 4.25 TKK251 pKa = 9.8 TKK253 pKa = 9.49 AGEE256 pKa = 4.08 RR257 pKa = 11.84 VVSIDD262 pKa = 3.05 KK263 pKa = 9.71 ATVLMLKK270 pKa = 9.98 QYY272 pKa = 8.73 QNRR275 pKa = 11.84 QRR277 pKa = 11.84 VVFLEE282 pKa = 4.08 VGGAPTRR289 pKa = 11.84 VFEE292 pKa = 4.45 TPTRR296 pKa = 11.84 LYY298 pKa = 9.59 LTRR301 pKa = 11.84 NNFQRR306 pKa = 11.84 VLDD309 pKa = 3.66 HH310 pKa = 6.85 HH311 pKa = 6.68 CEE313 pKa = 3.79 KK314 pKa = 11.27 LEE316 pKa = 3.84 IPRR319 pKa = 11.84 VTLHH323 pKa = 7.05 AFRR326 pKa = 11.84 HH327 pKa = 4.68 THH329 pKa = 7.12 ASLLLNAGISYY340 pKa = 10.65 KK341 pKa = 10.3 EE342 pKa = 3.81 LQHH345 pKa = 7.18 RR346 pKa = 11.84 LGHH349 pKa = 5.94 SNITLTLDD357 pKa = 3.69 TYY359 pKa = 11.66 SHH361 pKa = 7.12 LSKK364 pKa = 10.9 DD365 pKa = 3.57 KK366 pKa = 10.41 EE367 pKa = 4.25 KK368 pKa = 10.93 EE369 pKa = 3.85 AVSYY373 pKa = 9.71 YY374 pKa = 10.5 EE375 pKa = 4.33 KK376 pKa = 11.08 ALASLL381 pKa = 4.18
Molecular weight: 44.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.106
IPC2_protein 9.37
IPC_protein 9.311
Toseland 10.116
ProMoST 9.736
Dawson 10.292
Bjellqvist 9.926
Wikipedia 10.438
Rodwell 10.818
Grimsley 10.35
Solomon 10.306
Lehninger 10.277
Nozaki 10.087
DTASelect 9.926
Thurlkill 10.145
EMBOSS 10.511
Sillero 10.189
Patrickios 10.438
IPC_peptide 10.306
IPC2_peptide 8.419
IPC2.peptide.svr19 8.361
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18
0
18
3242
41
496
180.1
20.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.151 ± 0.554
0.401 ± 0.144
5.552 ± 0.725
8.822 ± 0.788
4.257 ± 0.293
4.349 ± 0.34
2.005 ± 0.244
7.465 ± 0.502
9.469 ± 0.42
10.672 ± 0.443
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.283 ± 0.296
6.231 ± 0.498
2.992 ± 0.453
4.627 ± 0.658
4.935 ± 0.422
5.891 ± 0.396
5.46 ± 0.492
4.226 ± 0.432
0.802 ± 0.144
4.411 ± 0.321
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here