Terriglobus albidus
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4970 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B9EGS6|A0A5B9EGS6_9BACT Glycerophosphodiester phosphodiesterase OS=Terriglobus albidus OX=1592106 GN=FTW19_21595 PE=4 SV=1
MM1 pKa = 8.02 RR2 pKa = 11.84 FPFVPVLLLLASSSILPAQTNLSFEE27 pKa = 4.62 LSRR30 pKa = 11.84 DD31 pKa = 3.1 TYY33 pKa = 10.07 MAFGVEE39 pKa = 4.98 AMAQGDD45 pKa = 4.07 FNGDD49 pKa = 3.51 GKK51 pKa = 10.23 PDD53 pKa = 3.47 MVFGGGSSFTVVTLRR68 pKa = 11.84 LGNGDD73 pKa = 3.66 GTFQPPITVGQADD86 pKa = 3.62 SSEE89 pKa = 4.54 VEE91 pKa = 4.87 DD92 pKa = 5.04 IAAADD97 pKa = 3.99 LNQDD101 pKa = 3.1 GKK103 pKa = 11.47 LDD105 pKa = 4.19 VIVLCIGGTFDD116 pKa = 4.19 VFLGNGDD123 pKa = 4.08 GSFQPAIAVATAGSPRR139 pKa = 11.84 AMAVGDD145 pKa = 3.74 FNGDD149 pKa = 3.35 QRR151 pKa = 11.84 PDD153 pKa = 3.32 LAIGDD158 pKa = 4.18 SQGSVEE164 pKa = 4.82 IFNNTDD170 pKa = 2.46 GKK172 pKa = 10.72 NFVLSNTVPIDD183 pKa = 3.36 PTRR186 pKa = 11.84 DD187 pKa = 3.16 ILNVKK192 pKa = 10.37 AGDD195 pKa = 3.79 FDD197 pKa = 6.21 DD198 pKa = 6.49 DD199 pKa = 4.23 GVTNLAVLTSDD210 pKa = 4.12 SAYY213 pKa = 10.86 ALWNDD218 pKa = 3.41 GAGNFRR224 pKa = 11.84 KK225 pKa = 9.82 SALSAYY231 pKa = 7.36 TNPAGLNVGDD241 pKa = 4.69 LNQDD245 pKa = 2.83 GMTDD249 pKa = 2.98 ILVSYY254 pKa = 9.63 DD255 pKa = 3.41 CHH257 pKa = 7.09 PNPQYY262 pKa = 10.17 PGPKK266 pKa = 10.06 GPISTCEE273 pKa = 3.98 GVDD276 pKa = 3.26 VFYY279 pKa = 10.88 GQGQQKK285 pKa = 7.79 TFEE288 pKa = 4.12 RR289 pKa = 11.84 HH290 pKa = 5.83 IITADD295 pKa = 3.19 TVGAARR301 pKa = 11.84 YY302 pKa = 8.47 LWAADD307 pKa = 3.61 VNGDD311 pKa = 4.11 GIGDD315 pKa = 4.64 LVTGTSDD322 pKa = 3.64 QNGSQTGLFIWLGHH336 pKa = 6.82 PDD338 pKa = 3.38 GSFDD342 pKa = 3.39 QAASRR347 pKa = 11.84 FIATSSGTGQLVVADD362 pKa = 4.81 FNRR365 pKa = 11.84 DD366 pKa = 3.03 GMIDD370 pKa = 4.09 FAQALPGDD378 pKa = 4.43 AQTQIYY384 pKa = 10.49 LNATNRR390 pKa = 11.84 APCATSQINPTVTVCQPVDD409 pKa = 3.6 NTYY412 pKa = 11.08 LPAPAIRR419 pKa = 11.84 VTANAYY425 pKa = 7.85 DD426 pKa = 4.14 TNQVTAMQLYY436 pKa = 9.42 VNGDD440 pKa = 3.66 LVYY443 pKa = 10.53 SRR445 pKa = 11.84 PVSGFNRR452 pKa = 11.84 TFQLGEE458 pKa = 3.84 GSFNLVTKK466 pKa = 10.17 AWDD469 pKa = 3.57 YY470 pKa = 11.69 AGLNFRR476 pKa = 11.84 SVRR479 pKa = 11.84 HH480 pKa = 3.9 VTLYY484 pKa = 10.8 NGTPGSVCAAALGTAEE500 pKa = 3.95 ICLPSGATSTSPVHH514 pKa = 6.31 IVGNGYY520 pKa = 7.88 TSYY523 pKa = 11.35 VPTAAQLYY531 pKa = 9.43 IDD533 pKa = 4.54 GNLVINNTSCNDD545 pKa = 3.28 YY546 pKa = 10.22 GACPGGSSLVDD557 pKa = 3.53 TYY559 pKa = 11.49 QGLSSGSHH567 pKa = 6.28 DD568 pKa = 4.94 LVFKK572 pKa = 10.94 LWDD575 pKa = 3.74 ANGNIYY581 pKa = 10.7 SADD584 pKa = 3.25 KK585 pKa = 9.88 TVSVEE590 pKa = 3.82
Molecular weight: 61.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.973
IPC_protein 4.024
Toseland 3.783
ProMoST 4.19
Dawson 4.037
Bjellqvist 4.202
Wikipedia 4.012
Rodwell 3.846
Grimsley 3.694
Solomon 4.037
Lehninger 3.999
Nozaki 4.139
DTASelect 4.469
Thurlkill 3.846
EMBOSS 4.012
Sillero 4.151
Patrickios 1.481
IPC_peptide 4.024
IPC2_peptide 4.126
IPC2.peptide.svr19 4.021
Protein with the highest isoelectric point:
>tr|A0A5B9E5V1|A0A5B9E5V1_9BACT Ribonuclease J OS=Terriglobus albidus OX=1592106 GN=rnj PE=3 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.02 RR4 pKa = 11.84 TFQPNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SKK15 pKa = 10.13 VHH17 pKa = 5.86 GFLSRR22 pKa = 11.84 MASKK26 pKa = 10.6 AGRR29 pKa = 11.84 AVLSRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.19 GRR40 pKa = 11.84 HH41 pKa = 5.72 KK42 pKa = 10.29 IAVSAGFRR50 pKa = 11.84 DD51 pKa = 3.66
Molecular weight: 5.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.441
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.369
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.102
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4970
0
4970
1854419
26
3063
373.1
40.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.626 ± 0.038
0.838 ± 0.01
4.963 ± 0.03
5.351 ± 0.044
3.916 ± 0.02
8.015 ± 0.037
2.249 ± 0.017
5.046 ± 0.024
3.758 ± 0.031
9.915 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.271 ± 0.016
3.511 ± 0.039
5.248 ± 0.022
3.986 ± 0.024
6.157 ± 0.039
6.446 ± 0.041
6.243 ± 0.049
7.204 ± 0.029
1.417 ± 0.015
2.841 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here