Amino acid dipepetide frequency for Sphingomonas phage vB_StuS_MMDA13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.068AlaAla: 13.068 ± 2.085
1.102AlaCys: 1.102 ± 0.256
5.708AlaAsp: 5.708 ± 0.471
5.908AlaGlu: 5.908 ± 0.842
3.405AlaPhe: 3.405 ± 0.51
9.313AlaGly: 9.313 ± 0.672
1.302AlaHis: 1.302 ± 0.218
4.657AlaIle: 4.657 ± 0.557
5.458AlaLys: 5.458 ± 0.828
8.512AlaLeu: 8.512 ± 0.629
3.004AlaMet: 3.004 ± 0.292
5.107AlaAsn: 5.107 ± 0.544
5.758AlaPro: 5.758 ± 1.136
5.207AlaGln: 5.207 ± 0.58
6.759AlaArg: 6.759 ± 0.706
5.007AlaSer: 5.007 ± 0.475
6.609AlaThr: 6.609 ± 0.778
6.209AlaVal: 6.209 ± 0.538
1.602AlaTrp: 1.602 ± 0.281
3.054AlaTyr: 3.054 ± 0.354
0.0AlaXaa: 0.0 ± 0.0
Cys
1.001CysAla: 1.001 ± 0.237
0.1CysCys: 0.1 ± 0.068
1.051CysAsp: 1.051 ± 0.171
0.651CysGlu: 0.651 ± 0.187
0.401CysPhe: 0.401 ± 0.121
1.152CysGly: 1.152 ± 0.348
0.35CysHis: 0.35 ± 0.118
0.401CysIle: 0.401 ± 0.138
0.601CysLys: 0.601 ± 0.174
0.501CysLeu: 0.501 ± 0.162
0.401CysMet: 0.401 ± 0.128
0.701CysAsn: 0.701 ± 0.198
0.651CysPro: 0.651 ± 0.22
0.451CysGln: 0.451 ± 0.168
0.751CysArg: 0.751 ± 0.202
0.35CysSer: 0.35 ± 0.152
0.401CysThr: 0.401 ± 0.126
0.701CysVal: 0.701 ± 0.225
0.15CysTrp: 0.15 ± 0.082
0.601CysTyr: 0.601 ± 0.153
0.0CysXaa: 0.0 ± 0.0
Asp
6.86AspAla: 6.86 ± 0.609
0.651AspCys: 0.651 ± 0.193
3.956AspAsp: 3.956 ± 0.441
4.056AspGlu: 4.056 ± 0.394
2.153AspPhe: 2.153 ± 0.368
5.257AspGly: 5.257 ± 0.507
1.502AspHis: 1.502 ± 0.24
2.854AspIle: 2.854 ± 0.348
2.954AspLys: 2.954 ± 0.319
5.658AspLeu: 5.658 ± 0.478
1.252AspMet: 1.252 ± 0.283
2.253AspAsn: 2.253 ± 0.347
3.905AspPro: 3.905 ± 0.399
2.003AspGln: 2.003 ± 0.264
2.954AspArg: 2.954 ± 0.386
2.754AspSer: 2.754 ± 0.328
3.605AspThr: 3.605 ± 0.467
3.255AspVal: 3.255 ± 0.402
0.851AspTrp: 0.851 ± 0.204
2.303AspTyr: 2.303 ± 0.326
0.0AspXaa: 0.0 ± 0.0
Glu
7.16GluAla: 7.16 ± 0.799
0.651GluCys: 0.651 ± 0.166
3.305GluAsp: 3.305 ± 0.372
3.705GluGlu: 3.705 ± 0.431
2.453GluPhe: 2.453 ± 0.333
4.406GluGly: 4.406 ± 0.453
1.352GluHis: 1.352 ± 0.292
3.305GluIle: 3.305 ± 0.406
2.904GluLys: 2.904 ± 0.516
5.307GluLeu: 5.307 ± 0.495
2.203GluMet: 2.203 ± 0.249
2.253GluAsn: 2.253 ± 0.281
1.903GluPro: 1.903 ± 0.341
3.455GluGln: 3.455 ± 0.436
3.956GluArg: 3.956 ± 0.536
3.154GluSer: 3.154 ± 0.371
2.654GluThr: 2.654 ± 0.321
4.006GluVal: 4.006 ± 0.457
1.001GluTrp: 1.001 ± 0.241
2.003GluTyr: 2.003 ± 0.322
0.0GluXaa: 0.0 ± 0.0
Phe
3.505PheAla: 3.505 ± 0.427
0.451PheCys: 0.451 ± 0.17
3.104PheAsp: 3.104 ± 0.425
2.303PheGlu: 2.303 ± 0.314
1.702PhePhe: 1.702 ± 0.279
3.405PheGly: 3.405 ± 0.375
0.551PheHis: 0.551 ± 0.157
1.702PheIle: 1.702 ± 0.24
2.253PheLys: 2.253 ± 0.363
2.904PheLeu: 2.904 ± 0.383
0.601PheMet: 0.601 ± 0.162
1.803PheAsn: 1.803 ± 0.24
1.552PhePro: 1.552 ± 0.297
1.202PheGln: 1.202 ± 0.206
2.253PheArg: 2.253 ± 0.352
1.752PheSer: 1.752 ± 0.271
2.704PheThr: 2.704 ± 0.416
2.654PheVal: 2.654 ± 0.474
0.601PheTrp: 0.601 ± 0.145
1.152PheTyr: 1.152 ± 0.213
0.0PheXaa: 0.0 ± 0.0
Gly
7.561GlyAla: 7.561 ± 0.713
1.252GlyCys: 1.252 ± 0.29
4.506GlyAsp: 4.506 ± 0.597
4.957GlyGlu: 4.957 ± 0.491
3.204GlyPhe: 3.204 ± 0.318
7.11GlyGly: 7.11 ± 0.878
1.752GlyHis: 1.752 ± 0.332
3.455GlyIle: 3.455 ± 0.34
4.306GlyLys: 4.306 ± 0.456
5.608GlyLeu: 5.608 ± 0.6
1.853GlyMet: 1.853 ± 0.287
3.555GlyAsn: 3.555 ± 0.374
3.004GlyPro: 3.004 ± 0.324
2.804GlyGln: 2.804 ± 0.38
3.505GlyArg: 3.505 ± 0.396
4.707GlySer: 4.707 ± 0.525
5.858GlyThr: 5.858 ± 0.756
5.458GlyVal: 5.458 ± 0.374
1.302GlyTrp: 1.302 ± 0.232
2.203GlyTyr: 2.203 ± 0.31
0.0GlyXaa: 0.0 ± 0.0
His
1.352HisAla: 1.352 ± 0.249
0.25HisCys: 0.25 ± 0.125
1.051HisAsp: 1.051 ± 0.201
0.801HisGlu: 0.801 ± 0.21
0.601HisPhe: 0.601 ± 0.242
1.402HisGly: 1.402 ± 0.212
0.551HisHis: 0.551 ± 0.193
1.502HisIle: 1.502 ± 0.305
1.252HisLys: 1.252 ± 0.24
1.502HisLeu: 1.502 ± 0.381
0.451HisMet: 0.451 ± 0.137
0.651HisAsn: 0.651 ± 0.142
1.302HisPro: 1.302 ± 0.235
0.501HisGln: 0.501 ± 0.148
1.302HisArg: 1.302 ± 0.294
1.001HisSer: 1.001 ± 0.228
1.502HisThr: 1.502 ± 0.224
1.001HisVal: 1.001 ± 0.229
0.701HisTrp: 0.701 ± 0.218
0.651HisTyr: 0.651 ± 0.172
0.0HisXaa: 0.0 ± 0.0
Ile
5.458IleAla: 5.458 ± 0.5
0.551IleCys: 0.551 ± 0.151
3.505IleAsp: 3.505 ± 0.381
3.455IleGlu: 3.455 ± 0.454
1.152IlePhe: 1.152 ± 0.269
3.855IleGly: 3.855 ± 0.352
0.451IleHis: 0.451 ± 0.125
2.303IleIle: 2.303 ± 0.325
2.303IleLys: 2.303 ± 0.338
3.455IleLeu: 3.455 ± 0.383
1.001IleMet: 1.001 ± 0.181
3.054IleAsn: 3.054 ± 0.416
2.353IlePro: 2.353 ± 0.355
2.203IleGln: 2.203 ± 0.49
2.904IleArg: 2.904 ± 0.356
2.303IleSer: 2.303 ± 0.287
2.954IleThr: 2.954 ± 0.406
3.305IleVal: 3.305 ± 0.332
0.701IleTrp: 0.701 ± 0.153
1.252IleTyr: 1.252 ± 0.209
0.0IleXaa: 0.0 ± 0.0
Lys
5.408LysAla: 5.408 ± 0.8
0.401LysCys: 0.401 ± 0.172
2.904LysAsp: 2.904 ± 0.337
3.004LysGlu: 3.004 ± 0.409
2.153LysPhe: 2.153 ± 0.271
3.405LysGly: 3.405 ± 0.44
1.152LysHis: 1.152 ± 0.298
2.403LysIle: 2.403 ± 0.261
2.453LysLys: 2.453 ± 0.333
4.106LysLeu: 4.106 ± 0.513
2.253LysMet: 2.253 ± 0.327
1.502LysAsn: 1.502 ± 0.288
2.654LysPro: 2.654 ± 0.374
2.053LysGln: 2.053 ± 0.284
3.104LysArg: 3.104 ± 0.383
2.754LysSer: 2.754 ± 0.375
2.604LysThr: 2.604 ± 0.364
3.905LysVal: 3.905 ± 0.435
0.751LysTrp: 0.751 ± 0.261
1.352LysTyr: 1.352 ± 0.259
0.0LysXaa: 0.0 ± 0.0
Leu
8.562LeuAla: 8.562 ± 0.875
0.901LeuCys: 0.901 ± 0.212
5.558LeuAsp: 5.558 ± 0.582
4.606LeuGlu: 4.606 ± 0.517
3.255LeuPhe: 3.255 ± 0.406
5.057LeuGly: 5.057 ± 0.654
1.953LeuHis: 1.953 ± 0.286
3.555LeuIle: 3.555 ± 0.342
3.755LeuLys: 3.755 ± 0.491
4.857LeuLeu: 4.857 ± 0.517
1.652LeuMet: 1.652 ± 0.234
3.405LeuAsn: 3.405 ± 0.387
4.206LeuPro: 4.206 ± 0.474
3.405LeuGln: 3.405 ± 0.398
4.757LeuArg: 4.757 ± 0.406
4.807LeuSer: 4.807 ± 0.421
5.458LeuThr: 5.458 ± 0.533
4.907LeuVal: 4.907 ± 0.489
0.951LeuTrp: 0.951 ± 0.214
2.203LeuTyr: 2.203 ± 0.329
0.0LeuXaa: 0.0 ± 0.0
Met
3.455MetAla: 3.455 ± 0.302
0.3MetCys: 0.3 ± 0.116
1.452MetAsp: 1.452 ± 0.244
2.303MetGlu: 2.303 ± 0.327
0.701MetPhe: 0.701 ± 0.176
1.402MetGly: 1.402 ± 0.238
0.601MetHis: 0.601 ± 0.198
1.051MetIle: 1.051 ± 0.212
1.152MetLys: 1.152 ± 0.23
2.303MetLeu: 2.303 ± 0.341
0.601MetMet: 0.601 ± 0.183
1.051MetAsn: 1.051 ± 0.199
1.502MetPro: 1.502 ± 0.291
1.051MetGln: 1.051 ± 0.207
1.903MetArg: 1.903 ± 0.3
2.003MetSer: 2.003 ± 0.272
1.552MetThr: 1.552 ± 0.294
1.953MetVal: 1.953 ± 0.289
0.401MetTrp: 0.401 ± 0.17
0.551MetTyr: 0.551 ± 0.163
0.0MetXaa: 0.0 ± 0.0
Asn
4.857AsnAla: 4.857 ± 0.586
0.35AsnCys: 0.35 ± 0.129
2.954AsnAsp: 2.954 ± 0.427
2.604AsnGlu: 2.604 ± 0.328
1.702AsnPhe: 1.702 ± 0.24
4.156AsnGly: 4.156 ± 0.482
0.451AsnHis: 0.451 ± 0.139
2.053AsnIle: 2.053 ± 0.369
1.853AsnLys: 1.853 ± 0.304
3.455AsnLeu: 3.455 ± 0.473
1.402AsnMet: 1.402 ± 0.257
2.353AsnAsn: 2.353 ± 0.298
2.854AsnPro: 2.854 ± 0.417
1.602AsnGln: 1.602 ± 0.288
2.353AsnArg: 2.353 ± 0.334
2.554AsnSer: 2.554 ± 0.339
2.754AsnThr: 2.754 ± 0.376
2.754AsnVal: 2.754 ± 0.376
0.551AsnTrp: 0.551 ± 0.158
1.252AsnTyr: 1.252 ± 0.243
0.0AsnXaa: 0.0 ± 0.0
Pro
6.709ProAla: 6.709 ± 1.18
0.3ProCys: 0.3 ± 0.126
3.655ProAsp: 3.655 ± 0.441
3.605ProGlu: 3.605 ± 0.462
2.353ProPhe: 2.353 ± 0.389
3.505ProGly: 3.505 ± 0.548
0.951ProHis: 0.951 ± 0.188
1.803ProIle: 1.803 ± 0.33
2.604ProLys: 2.604 ± 0.376
4.156ProLeu: 4.156 ± 0.511
1.302ProMet: 1.302 ± 0.235
2.303ProAsn: 2.303 ± 0.35
3.104ProPro: 3.104 ± 0.348
1.652ProGln: 1.652 ± 0.298
2.203ProArg: 2.203 ± 0.272
2.003ProSer: 2.003 ± 0.284
3.104ProThr: 3.104 ± 0.415
4.056ProVal: 4.056 ± 0.469
1.001ProTrp: 1.001 ± 0.25
1.352ProTyr: 1.352 ± 0.35
0.0ProXaa: 0.0 ± 0.0
Gln
4.006GlnAla: 4.006 ± 0.789
0.401GlnCys: 0.401 ± 0.15
2.203GlnAsp: 2.203 ± 0.3
2.504GlnGlu: 2.504 ± 0.301
1.552GlnPhe: 1.552 ± 0.262
3.104GlnGly: 3.104 ± 0.391
0.451GlnHis: 0.451 ± 0.13
1.953GlnIle: 1.953 ± 0.343
1.853GlnLys: 1.853 ± 0.318
3.855GlnLeu: 3.855 ± 0.434
1.152GlnMet: 1.152 ± 0.229
1.752GlnAsn: 1.752 ± 0.286
1.853GlnPro: 1.853 ± 0.324
2.153GlnGln: 2.153 ± 0.669
3.355GlnArg: 3.355 ± 0.439
1.803GlnSer: 1.803 ± 0.313
2.353GlnThr: 2.353 ± 0.365
2.504GlnVal: 2.504 ± 0.367
0.451GlnTrp: 0.451 ± 0.17
1.202GlnTyr: 1.202 ± 0.218
0.0GlnXaa: 0.0 ± 0.0
Arg
5.257ArgAla: 5.257 ± 0.473
0.551ArgCys: 0.551 ± 0.178
2.554ArgAsp: 2.554 ± 0.349
4.657ArgGlu: 4.657 ± 0.711
1.652ArgPhe: 1.652 ± 0.26
3.705ArgGly: 3.705 ± 0.489
1.051ArgHis: 1.051 ± 0.203
2.554ArgIle: 2.554 ± 0.319
4.657ArgLys: 4.657 ± 0.595
4.707ArgLeu: 4.707 ± 0.51
1.803ArgMet: 1.803 ± 0.272
2.654ArgAsn: 2.654 ± 0.321
2.604ArgPro: 2.604 ± 0.341
2.303ArgGln: 2.303 ± 0.312
3.655ArgArg: 3.655 ± 0.468
2.654ArgSer: 2.654 ± 0.342
2.804ArgThr: 2.804 ± 0.362
4.106ArgVal: 4.106 ± 0.418
1.302ArgTrp: 1.302 ± 0.251
1.652ArgTyr: 1.652 ± 0.315
0.0ArgXaa: 0.0 ± 0.0
Ser
5.508SerAla: 5.508 ± 0.456
0.35SerCys: 0.35 ± 0.138
2.904SerAsp: 2.904 ± 0.377
2.403SerGlu: 2.403 ± 0.339
2.153SerPhe: 2.153 ± 0.263
4.707SerGly: 4.707 ± 0.552
1.452SerHis: 1.452 ± 0.297
3.255SerIle: 3.255 ± 0.377
2.403SerLys: 2.403 ± 0.323
3.505SerLeu: 3.505 ± 0.461
1.853SerMet: 1.853 ± 0.251
2.453SerAsn: 2.453 ± 0.395
2.554SerPro: 2.554 ± 0.358
1.652SerGln: 1.652 ± 0.312
2.403SerArg: 2.403 ± 0.327
3.405SerSer: 3.405 ± 0.451
2.954SerThr: 2.954 ± 0.508
3.505SerVal: 3.505 ± 0.495
1.102SerTrp: 1.102 ± 0.215
2.053SerTyr: 2.053 ± 0.307
0.0SerXaa: 0.0 ± 0.0
Thr
6.159ThrAla: 6.159 ± 0.791
0.851ThrCys: 0.851 ± 0.204
3.505ThrAsp: 3.505 ± 0.453
2.804ThrGlu: 2.804 ± 0.356
2.854ThrPhe: 2.854 ± 0.337
6.259ThrGly: 6.259 ± 0.594
1.452ThrHis: 1.452 ± 0.318
3.305ThrIle: 3.305 ± 0.588
1.752ThrLys: 1.752 ± 0.288
5.107ThrLeu: 5.107 ± 0.531
1.402ThrMet: 1.402 ± 0.273
2.253ThrAsn: 2.253 ± 0.276
3.956ThrPro: 3.956 ± 0.461
2.403ThrGln: 2.403 ± 0.372
2.103ThrArg: 2.103 ± 0.288
3.405ThrSer: 3.405 ± 0.462
3.705ThrThr: 3.705 ± 0.411
4.056ThrVal: 4.056 ± 0.493
1.102ThrTrp: 1.102 ± 0.284
2.153ThrTyr: 2.153 ± 0.312
0.0ThrXaa: 0.0 ± 0.0
Val
6.309ValAla: 6.309 ± 0.556
0.951ValCys: 0.951 ± 0.224
4.356ValAsp: 4.356 ± 0.448
4.406ValGlu: 4.406 ± 0.465
2.554ValPhe: 2.554 ± 0.377
4.356ValGly: 4.356 ± 0.387
1.402ValHis: 1.402 ± 0.278
3.905ValIle: 3.905 ± 0.486
3.956ValLys: 3.956 ± 0.41
4.606ValLeu: 4.606 ± 0.496
1.652ValMet: 1.652 ± 0.245
3.255ValAsn: 3.255 ± 0.419
3.505ValPro: 3.505 ± 0.457
2.554ValGln: 2.554 ± 0.342
3.455ValArg: 3.455 ± 0.4
3.405ValSer: 3.405 ± 0.387
4.356ValThr: 4.356 ± 0.494
5.257ValVal: 5.257 ± 0.546
0.701ValTrp: 0.701 ± 0.197
1.652ValTyr: 1.652 ± 0.26
0.0ValXaa: 0.0 ± 0.0
Trp
1.602TrpAla: 1.602 ± 0.317
0.35TrpCys: 0.35 ± 0.121
0.851TrpAsp: 0.851 ± 0.176
0.951TrpGlu: 0.951 ± 0.22
0.751TrpPhe: 0.751 ± 0.186
0.851TrpGly: 0.851 ± 0.184
0.25TrpHis: 0.25 ± 0.115
0.551TrpIle: 0.551 ± 0.185
0.651TrpLys: 0.651 ± 0.167
1.552TrpLeu: 1.552 ± 0.284
0.601TrpMet: 0.601 ± 0.142
0.951TrpAsn: 0.951 ± 0.215
0.901TrpPro: 0.901 ± 0.246
0.701TrpGln: 0.701 ± 0.192
0.901TrpArg: 0.901 ± 0.186
0.851TrpSer: 0.851 ± 0.17
0.951TrpThr: 0.951 ± 0.209
0.701TrpVal: 0.701 ± 0.153
0.601TrpTrp: 0.601 ± 0.165
0.851TrpTyr: 0.851 ± 0.251
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.954TyrAla: 2.954 ± 0.425
0.651TyrCys: 0.651 ± 0.178
1.953TyrAsp: 1.953 ± 0.324
1.502TyrGlu: 1.502 ± 0.253
1.402TyrPhe: 1.402 ± 0.282
1.552TyrGly: 1.552 ± 0.242
0.35TyrHis: 0.35 ± 0.132
1.953TyrIle: 1.953 ± 0.341
1.202TyrLys: 1.202 ± 0.214
2.153TyrLeu: 2.153 ± 0.282
0.801TyrMet: 0.801 ± 0.22
1.552TyrAsn: 1.552 ± 0.251
1.602TyrPro: 1.602 ± 0.274
1.102TyrGln: 1.102 ± 0.186
2.303TyrArg: 2.303 ± 0.299
1.903TyrSer: 1.903 ± 0.334
1.702TyrThr: 1.702 ± 0.33
2.303TyrVal: 2.303 ± 0.369
0.551TyrTrp: 0.551 ± 0.135
0.801TyrTyr: 0.801 ± 0.189
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 89 proteins (19973 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski