Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R)
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2105 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8DX87|Q8DX87_STRA5 AAA_28 domain-containing protein OS=Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R) OX=208435 GN=SAG1967 PE=4 SV=1
MM1 pKa = 7.77 DD2 pKa = 3.45 VSAIVRR8 pKa = 11.84 DD9 pKa = 3.8 IKK11 pKa = 10.04 TGRR14 pKa = 11.84 ATLVCFPVEE23 pKa = 3.91 MSEE26 pKa = 4.73 LKK28 pKa = 10.64 LALGLRR34 pKa = 11.84 EE35 pKa = 4.3 EE36 pKa = 5.57 DD37 pKa = 3.4 DD38 pKa = 3.98 LEE40 pKa = 4.41 YY41 pKa = 10.35 IIADD45 pKa = 3.94 SDD47 pKa = 3.94 CQLLKK52 pKa = 10.68 EE53 pKa = 4.33 HH54 pKa = 7.47 DD55 pKa = 4.7 SIEE58 pKa = 4.4 MINQFVEE65 pKa = 4.26 LVEE68 pKa = 4.63 NVDD71 pKa = 3.88 SEE73 pKa = 4.57 LVKK76 pKa = 10.64 AVHH79 pKa = 5.79 QVIGYY84 pKa = 5.47 TASDD88 pKa = 3.51 FVDD91 pKa = 3.22 YY92 pKa = 11.17 DD93 pKa = 4.3 FNFGDD98 pKa = 4.39 CCLLSDD104 pKa = 3.56 VTTRR108 pKa = 11.84 RR109 pKa = 11.84 EE110 pKa = 3.62 LGEE113 pKa = 4.23 YY114 pKa = 10.48 YY115 pKa = 10.31 FDD117 pKa = 3.64 EE118 pKa = 5.54 LGVQGVGKK126 pKa = 9.59 EE127 pKa = 3.89 ALEE130 pKa = 4.24 MYY132 pKa = 9.98 FDD134 pKa = 3.9 HH135 pKa = 6.98 EE136 pKa = 4.57 AYY138 pKa = 10.38 GRR140 pKa = 11.84 DD141 pKa = 3.35 IDD143 pKa = 5.43 LEE145 pKa = 4.4 SQGGFSDD152 pKa = 3.57 YY153 pKa = 11.1 GYY155 pKa = 11.18 VEE157 pKa = 3.81 ISGG160 pKa = 3.66
Molecular weight: 18.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.948
IPC_protein 3.923
Toseland 3.732
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.808
Rodwell 3.757
Grimsley 3.643
Solomon 3.897
Lehninger 3.846
Nozaki 4.012
DTASelect 4.202
Thurlkill 3.77
EMBOSS 3.821
Sillero 4.05
Patrickios 1.163
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.917
Protein with the highest isoelectric point:
>sp|Q8DXR8|RSMA_STRA5 Ribosomal RNA small subunit methyltransferase A OS=Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R) OX=208435 GN=rsmA PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.67 IRR11 pKa = 11.84 RR12 pKa = 11.84 QRR14 pKa = 11.84 KK15 pKa = 7.52 HH16 pKa = 5.2 GFRR19 pKa = 11.84 HH20 pKa = 6.39 RR21 pKa = 11.84 MSTKK25 pKa = 9.02 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2105
0
2105
616913
23
2274
293.1
32.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.904 ± 0.061
0.581 ± 0.016
5.644 ± 0.052
6.516 ± 0.059
4.571 ± 0.044
6.339 ± 0.051
1.981 ± 0.025
7.892 ± 0.055
7.339 ± 0.058
9.994 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.561 ± 0.031
4.924 ± 0.04
3.187 ± 0.029
3.976 ± 0.034
3.882 ± 0.039
6.55 ± 0.05
5.738 ± 0.047
6.728 ± 0.045
0.853 ± 0.016
3.839 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here