Anditalea andensis
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4341 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A074LKG2|A0A074LKG2_9BACT Amidohydrolase OS=Anditalea andensis OX=1048983 GN=EL17_09345 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.42 RR3 pKa = 11.84 STYY6 pKa = 10.6 LKK8 pKa = 10.33 ASFGIALSLLVFTSCDD24 pKa = 3.48 DD25 pKa = 3.89 TLDD28 pKa = 4.1 GPQAIADD35 pKa = 3.93 FTYY38 pKa = 10.64 EE39 pKa = 4.46 ADD41 pKa = 4.17 PNDD44 pKa = 3.82 DD45 pKa = 3.51 MTIHH49 pKa = 6.1 FTNTSQHH56 pKa = 5.3 HH57 pKa = 6.36 HH58 pKa = 5.37 SSYY61 pKa = 10.7 WLFGDD66 pKa = 4.62 GSDD69 pKa = 4.82 HH70 pKa = 7.38 SSEE73 pKa = 4.91 DD74 pKa = 3.87 SPTHH78 pKa = 5.7 TFPAGGIYY86 pKa = 9.17 EE87 pKa = 4.27 VKK89 pKa = 10.13 LAVMGNGTGNEE100 pKa = 3.84 IVRR103 pKa = 11.84 EE104 pKa = 3.85 VSVIDD109 pKa = 3.8 RR110 pKa = 11.84 SLLGEE115 pKa = 5.01 RR116 pKa = 11.84 IQDD119 pKa = 3.59 GNFEE123 pKa = 4.55 NPEE126 pKa = 3.75 NWNVYY131 pKa = 9.87 AGGSVEE137 pKa = 4.32 VTQHH141 pKa = 5.92 AFEE144 pKa = 4.71 NGQLILSNGEE154 pKa = 3.94 EE155 pKa = 4.18 VEE157 pKa = 4.63 SNVVVWQAIEE167 pKa = 4.03 VQANTDD173 pKa = 3.81 YY174 pKa = 11.4 VFSAQISGGGMHH186 pKa = 6.01 QSWVEE191 pKa = 3.6 FLFGDD196 pKa = 4.92 DD197 pKa = 4.75 EE198 pKa = 4.71 PEE200 pKa = 4.09 EE201 pKa = 5.48 DD202 pKa = 6.02 DD203 pKa = 6.28 DD204 pKa = 6.12 YY205 pKa = 11.96 SDD207 pKa = 4.68 NNQFSMNTWAEE218 pKa = 4.01 CGIDD222 pKa = 3.59 PFEE225 pKa = 4.7 GNIVEE230 pKa = 4.11 IHH232 pKa = 6.28 CAGDD236 pKa = 3.64 GEE238 pKa = 4.69 TDD240 pKa = 4.03 GIVNFEE246 pKa = 4.24 EE247 pKa = 5.73 GGTKK251 pKa = 10.23 YY252 pKa = 10.2 FAIKK256 pKa = 9.95 AGSWEE261 pKa = 4.26 GTLGPNGVTISNVSLIPADD280 pKa = 3.77 EE281 pKa = 4.08 LL282 pKa = 3.92
Molecular weight: 30.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.91
IPC_protein 3.897
Toseland 3.706
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.77
Rodwell 3.732
Grimsley 3.617
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.164
Thurlkill 3.732
EMBOSS 3.783
Sillero 4.012
Patrickios 1.252
IPC_peptide 3.859
IPC2_peptide 3.999
IPC2.peptide.svr19 3.898
Protein with the highest isoelectric point:
>tr|A0A074KVW1|A0A074KVW1_9BACT Histidine kinase OS=Anditalea andensis OX=1048983 GN=EL17_16810 PE=4 SV=1
MM1 pKa = 7.65 KK2 pKa = 10.06 NGKK5 pKa = 7.78 WASDD9 pKa = 3.32 YY10 pKa = 10.74 HH11 pKa = 6.24 RR12 pKa = 11.84 HH13 pKa = 6.3 NIINNIRR20 pKa = 11.84 ACQHH24 pKa = 5.25 FTRR27 pKa = 11.84 MHH29 pKa = 5.63 YY30 pKa = 9.81 AWFYY34 pKa = 10.14 RR35 pKa = 11.84 GRR37 pKa = 11.84 SSGSPDD43 pKa = 3.48 FRR45 pKa = 11.84 WPSHH49 pKa = 4.51 TQRR52 pKa = 11.84 CTVALFYY59 pKa = 11.19 LKK61 pKa = 10.24 FFAGLQQRR69 pKa = 11.84 GLLRR73 pKa = 11.84 FFTGFPFKK81 pKa = 11.17 AMASDD86 pKa = 4.52 ASPPMM91 pKa = 4.48
Molecular weight: 10.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 10.014
IPC_protein 10.906
Toseland 10.789
ProMoST 10.613
Dawson 10.906
Bjellqvist 10.687
Wikipedia 11.169
Rodwell 11.052
Grimsley 10.979
Solomon 11.052
Lehninger 11.008
Nozaki 10.789
DTASelect 10.672
Thurlkill 10.804
EMBOSS 11.199
Sillero 10.847
Patrickios 10.804
IPC_peptide 11.052
IPC2_peptide 9.897
IPC2.peptide.svr19 8.152
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4341
0
4341
1527423
39
2987
351.9
39.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.536 ± 0.034
0.66 ± 0.011
5.576 ± 0.024
6.568 ± 0.03
5.101 ± 0.025
6.993 ± 0.035
2.097 ± 0.02
7.802 ± 0.031
6.433 ± 0.05
9.53 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.543 ± 0.016
5.496 ± 0.031
3.863 ± 0.022
3.597 ± 0.021
4.229 ± 0.027
6.397 ± 0.025
5.2 ± 0.025
6.04 ± 0.028
1.221 ± 0.013
4.118 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here