Trichoderma gamsii
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11178 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P4ZKP7|A0A2P4ZKP7_9HYPO Uncharacterized protein OS=Trichoderma gamsii OX=398673 GN=TGAM01_v206395 PE=4 SV=1
MM1 pKa = 7.13 RR2 pKa = 11.84 TSAFAAILGFAVYY15 pKa = 10.09 GSQATAINFADD26 pKa = 3.38 NWRR29 pKa = 11.84 DD30 pKa = 2.95 NWRR33 pKa = 11.84 DD34 pKa = 3.1 NWPTNWPDD42 pKa = 2.89 NWSDD46 pKa = 3.15 NWPDD50 pKa = 3.11 NWPVTWLVSLLGSLNSLNRR69 pKa = 11.84 IDD71 pKa = 4.53 NLNSIDD77 pKa = 4.23 SLSSLDD83 pKa = 4.16 SLDD86 pKa = 3.83 SLASLASLNGLDD98 pKa = 4.57 HH99 pKa = 7.02 FPEE102 pKa = 5.02 NPCEE106 pKa = 4.06 WLGGTCEE113 pKa = 3.7 ATAVNFCSGLNDD125 pKa = 3.25 IKK127 pKa = 10.87 IGVPCGSFGSISVGTAGVGCCWHH150 pKa = 6.73 AAII153 pKa = 5.57
Molecular weight: 16.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.948
IPC_protein 3.923
Toseland 3.694
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.91
Rodwell 3.745
Grimsley 3.605
Solomon 3.923
Lehninger 3.872
Nozaki 4.062
DTASelect 4.342
Thurlkill 3.77
EMBOSS 3.923
Sillero 4.05
Patrickios 0.896
IPC_peptide 3.91
IPC2_peptide 4.012
IPC2.peptide.svr19 3.915
Protein with the highest isoelectric point:
>tr|A0A2P4Z7J2|A0A2P4Z7J2_9HYPO FAD-binding PCMH-type domain-containing protein OS=Trichoderma gamsii OX=398673 GN=TGAM01_v210894 PE=3 SV=1
AA1 pKa = 7.63 ALARR5 pKa = 11.84 AHH7 pKa = 5.78 TAGLGPHH14 pKa = 7.09 RR15 pKa = 11.84 GRR17 pKa = 11.84 SKK19 pKa = 11.0 LSRR22 pKa = 11.84 KK23 pKa = 9.05 PPRR26 pKa = 11.84 SWTRR30 pKa = 11.84 RR31 pKa = 11.84 SRR33 pKa = 11.84 VMLRR37 pKa = 11.84 LAGRR41 pKa = 11.84 TTKK44 pKa = 10.58 GSPTHH49 pKa = 6.25
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.574
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.31
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.172
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11178
0
11178
5506319
26
21960
492.6
54.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.885 ± 0.022
1.226 ± 0.009
5.774 ± 0.017
6.133 ± 0.025
3.765 ± 0.014
6.77 ± 0.024
2.359 ± 0.01
5.147 ± 0.017
4.915 ± 0.021
8.947 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.191 ± 0.009
3.778 ± 0.013
5.826 ± 0.026
4.07 ± 0.014
5.868 ± 0.024
8.339 ± 0.025
5.779 ± 0.016
5.985 ± 0.019
1.474 ± 0.008
2.768 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here