Morganella phage vB_MmoP_MP2
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A192Y9X7|A0A192Y9X7_9CAUD Uncharacterized protein OS=Morganella phage vB_MmoP_MP2 OX=1852627 GN=MP2_gp14 PE=4 SV=1
MM1 pKa = 6.31 TTYY4 pKa = 11.2 NEE6 pKa = 4.38 LLDD9 pKa = 3.82 TARR12 pKa = 11.84 DD13 pKa = 3.71 EE14 pKa = 4.59 LKK16 pKa = 10.74 DD17 pKa = 4.14 AIRR20 pKa = 11.84 EE21 pKa = 4.01 LHH23 pKa = 5.57 VTDD26 pKa = 5.22 EE27 pKa = 4.6 RR28 pKa = 11.84 DD29 pKa = 3.04 DD30 pKa = 4.84 DD31 pKa = 3.72 IRR33 pKa = 11.84 DD34 pKa = 3.7 RR35 pKa = 11.84 IYY37 pKa = 10.81 EE38 pKa = 4.27 IIEE41 pKa = 3.94 GAMPYY46 pKa = 10.52 ANHH49 pKa = 7.12 EE50 pKa = 4.07 IFEE53 pKa = 4.41 VFAGNGISYY62 pKa = 10.8 QMDD65 pKa = 3.96 DD66 pKa = 3.07 NGLIEE71 pKa = 4.67 GCTDD75 pKa = 3.26 VIEE78 pKa = 4.11 ILKK81 pKa = 8.91 MRR83 pKa = 11.84 IYY85 pKa = 10.91 EE86 pKa = 4.09 EE87 pKa = 4.59 LSNDD91 pKa = 3.52 LYY93 pKa = 11.74 GEE95 pKa = 4.19 LSDD98 pKa = 6.59 LIQEE102 pKa = 4.49 YY103 pKa = 10.85 NDD105 pKa = 3.58 EE106 pKa = 4.83 LEE108 pKa = 5.83 DD109 pKa = 3.65 EE110 pKa = 5.4 AEE112 pKa = 4.17 TCGYY116 pKa = 10.93 GDD118 pKa = 5.16 DD119 pKa = 5.78 DD120 pKa = 5.01 EE121 pKa = 6.58 EE122 pKa = 5.58 EE123 pKa = 4.49 DD124 pKa = 5.47 DD125 pKa = 4.14 EE126 pKa = 4.87
Molecular weight: 14.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.617
IPC_protein 3.617
Toseland 3.414
ProMoST 3.757
Dawson 3.592
Bjellqvist 3.783
Wikipedia 3.516
Rodwell 3.439
Grimsley 3.312
Solomon 3.579
Lehninger 3.541
Nozaki 3.706
DTASelect 3.897
Thurlkill 3.452
EMBOSS 3.528
Sillero 3.732
Patrickios 1.024
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.688
Protein with the highest isoelectric point:
>tr|A0A192YB31|A0A192YB31_9CAUD Internal virion protein D OS=Morganella phage vB_MmoP_MP2 OX=1852627 GN=MP2_gp45 PE=4 SV=1
MM1 pKa = 7.04 LQGVKK6 pKa = 10.38 SFTKK10 pKa = 10.32 RR11 pKa = 11.84 LLSSKK16 pKa = 10.09 LLRR19 pKa = 11.84 GFLYY23 pKa = 10.26 VSCPTVTRR31 pKa = 11.84 PLVTDD36 pKa = 4.09
Molecular weight: 4.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.736
IPC_protein 10.218
Toseland 10.716
ProMoST 10.277
Dawson 10.804
Bjellqvist 10.452
Wikipedia 10.95
Rodwell 11.228
Grimsley 10.833
Solomon 10.877
Lehninger 10.862
Nozaki 10.701
DTASelect 10.438
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.716
Patrickios 11.082
IPC_peptide 10.891
IPC2_peptide 9.443
IPC2.peptide.svr19 8.512
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
11987
35
1309
217.9
24.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.593 ± 0.407
0.968 ± 0.181
6.248 ± 0.302
7.516 ± 0.445
3.721 ± 0.19
7.441 ± 0.365
1.744 ± 0.174
5.314 ± 0.233
6.933 ± 0.381
7.859 ± 0.309
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.128 ± 0.191
4.572 ± 0.264
3.654 ± 0.213
3.787 ± 0.359
5.181 ± 0.173
6.065 ± 0.323
5.598 ± 0.256
6.399 ± 0.26
1.502 ± 0.175
3.779 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here