Pseudomonas phage pf16

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Chakrabartyvirus; Pseudomonas virus pf16

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5R3X7|A0A1S5R3X7_9CAUD Uncharacterized protein OS=Pseudomonas phage pf16 OX=1815630 GN=pf16_102 PE=4 SV=1
MM1 pKa = 7.15ITLKK5 pKa = 10.53EE6 pKa = 4.44HH7 pKa = 5.71IQITQAVQLMEE18 pKa = 4.45GEE20 pKa = 4.66SPVFTPEE27 pKa = 3.79QLEE30 pKa = 4.44CAASYY35 pKa = 9.94ALKK38 pKa = 9.87WNPITGMTALWLYY51 pKa = 10.97DD52 pKa = 4.45DD53 pKa = 4.97KK54 pKa = 11.84NQGLGSMEE62 pKa = 4.36FEE64 pKa = 5.15DD65 pKa = 4.6PDD67 pKa = 3.71VAIGFVEE74 pKa = 5.15EE75 pKa = 5.08YY76 pKa = 10.77FDD78 pKa = 6.69LDD80 pKa = 5.12DD81 pKa = 5.3EE82 pKa = 4.91DD83 pKa = 5.48LADD86 pKa = 4.06LEE88 pKa = 4.74SYY90 pKa = 10.69AWANDD95 pKa = 3.35NYY97 pKa = 11.06DD98 pKa = 4.99DD99 pKa = 4.77RR100 pKa = 11.84VQDD103 pKa = 3.89ALADD107 pKa = 4.47DD108 pKa = 4.52GTSVV112 pKa = 3.42

Molecular weight: 12.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5R3F9|A0A1S5R3F9_9CAUD Uncharacterized protein OS=Pseudomonas phage pf16 OX=1815630 GN=pf16_06 PE=4 SV=1
MM1 pKa = 6.77NTVEE5 pKa = 5.41EE6 pKa = 4.03RR7 pKa = 11.84RR8 pKa = 11.84AWYY11 pKa = 9.5IEE13 pKa = 3.71QFGSVRR19 pKa = 11.84HH20 pKa = 4.84WALYY24 pKa = 10.1CVTANWRR31 pKa = 11.84IRR33 pKa = 11.84KK34 pKa = 9.06RR35 pKa = 11.84LPRR38 pKa = 11.84LTARR42 pKa = 11.84AKK44 pKa = 9.43QLGVIEE50 pKa = 4.25

Molecular weight: 6.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

237

0

237

48767

38

1635

205.8

23.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.01 ± 0.161

1.128 ± 0.076

6.273 ± 0.114

6.556 ± 0.18

4.37 ± 0.109

6.504 ± 0.154

2.014 ± 0.114

5.875 ± 0.107

6.588 ± 0.163

7.909 ± 0.129

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.762 ± 0.084

5.051 ± 0.139

3.734 ± 0.107

3.632 ± 0.107

4.802 ± 0.129

6.02 ± 0.151

6.068 ± 0.194

7.708 ± 0.17

1.185 ± 0.056

3.812 ± 0.099
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski