Terfezia boudieri ATCC MYA-4762
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12026 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N4LIW4|A0A3N4LIW4_9PEZI Kinase-like protein OS=Terfezia boudieri ATCC MYA-4762 OX=1051890 GN=L211DRAFT_841522 PE=4 SV=1
MM1 pKa = 7.5 TPPIPPQEE9 pKa = 4.49 SQNSEE14 pKa = 3.82 QPEE17 pKa = 4.18 FQSLSLTDD25 pKa = 3.89 SKK27 pKa = 11.45 SSPKK31 pKa = 10.24 LLPRR35 pKa = 11.84 VLQDD39 pKa = 3.0 TDD41 pKa = 3.95 LVQPSGGAVSEE52 pKa = 4.21 ISNSGEE58 pKa = 3.79 SDD60 pKa = 3.59 SEE62 pKa = 4.24 SDD64 pKa = 3.92 SEE66 pKa = 5.21 SDD68 pKa = 3.61 TEE70 pKa = 4.85 SYY72 pKa = 10.36 SADD75 pKa = 3.57 YY76 pKa = 10.11 EE77 pKa = 4.44 SHH79 pKa = 6.0 YY80 pKa = 10.74 EE81 pKa = 3.91 SYY83 pKa = 10.83 YY84 pKa = 10.88 EE85 pKa = 3.99 SDD87 pKa = 3.43 EE88 pKa = 4.34 VSDD91 pKa = 4.7 DD92 pKa = 3.7 EE93 pKa = 4.98 SSGVSDD99 pKa = 3.94 YY100 pKa = 11.54 EE101 pKa = 4.05 SDD103 pKa = 3.93 EE104 pKa = 4.53 EE105 pKa = 4.5 SDD107 pKa = 4.04 CLSEE111 pKa = 4.04 EE112 pKa = 4.06 LGYY115 pKa = 9.64 TSTEE119 pKa = 4.21 SEE121 pKa = 4.57 GEE123 pKa = 4.25 SHH125 pKa = 6.92 SDD127 pKa = 4.28 SGDD130 pKa = 3.39 TSDD133 pKa = 5.01 DD134 pKa = 3.68 TEE136 pKa = 4.54 SDD138 pKa = 3.95 YY139 pKa = 11.6 EE140 pKa = 4.22 SDD142 pKa = 4.0 SEE144 pKa = 4.35 YY145 pKa = 10.64 GTLGSSEE152 pKa = 4.39 DD153 pKa = 3.81 SEE155 pKa = 5.51 LEE157 pKa = 3.75 QSGSEE162 pKa = 4.03 EE163 pKa = 4.27 SEE165 pKa = 4.16 SGSEE169 pKa = 5.02 DD170 pKa = 3.46 GRR172 pKa = 11.84 THH174 pKa = 5.67 QQLAEE179 pKa = 3.99 QTKK182 pKa = 10.57 SIVTLEE188 pKa = 4.68 CSPQLDD194 pKa = 3.34 KK195 pKa = 11.47 GDD197 pKa = 3.99 RR198 pKa = 11.84 EE199 pKa = 4.5 GCRR202 pKa = 11.84 RR203 pKa = 11.84 ASGTFHH209 pKa = 7.24 EE210 pKa = 4.89 RR211 pKa = 11.84 QQQ213 pKa = 3.28
Molecular weight: 23.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.681
IPC_protein 3.668
Toseland 3.465
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.528
Rodwell 3.49
Grimsley 3.376
Solomon 3.617
Lehninger 3.579
Nozaki 3.745
DTASelect 3.91
Thurlkill 3.503
EMBOSS 3.541
Sillero 3.783
Patrickios 1.151
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.699
Protein with the highest isoelectric point:
>tr|A0A3N4LER0|A0A3N4LER0_9PEZI Uncharacterized protein OS=Terfezia boudieri ATCC MYA-4762 OX=1051890 GN=L211DRAFT_852802 PE=4 SV=1
MM1 pKa = 7.59 LLLRR5 pKa = 11.84 LFRR8 pKa = 11.84 MRR10 pKa = 11.84 QMQLRR15 pKa = 11.84 LHH17 pKa = 6.21 KK18 pKa = 8.68 TQLMLQHH25 pKa = 6.07 KK26 pKa = 6.52 TQLRR30 pKa = 11.84 LQHH33 pKa = 5.62 KK34 pKa = 6.32 TQLRR38 pKa = 11.84 LQHH41 pKa = 5.57 KK42 pKa = 5.72 TQHH45 pKa = 5.79 RR46 pKa = 11.84 MQHH49 pKa = 4.36 KK50 pKa = 7.6 TKK52 pKa = 10.81 LRR54 pKa = 11.84 MQHH57 pKa = 5.08 KK58 pKa = 7.47 TQLRR62 pKa = 11.84 MQHH65 pKa = 4.94 YY66 pKa = 9.5 HH67 pKa = 6.28 YY68 pKa = 10.21 LTIFIFLLRR77 pKa = 11.84 FLLKK81 pKa = 10.59 HH82 pKa = 5.39 SLTRR86 pKa = 11.84 LLMQLSPTLRR96 pKa = 11.84 LPRR99 pKa = 11.84 LLRR102 pKa = 11.84 TLRR105 pKa = 11.84 LPRR108 pKa = 11.84 LLRR111 pKa = 11.84 TLRR114 pKa = 11.84 LPRR117 pKa = 11.84 LLRR120 pKa = 11.84 LPRR123 pKa = 11.84 LLRR126 pKa = 11.84 PRR128 pKa = 11.84 LLRR131 pKa = 11.84 KK132 pKa = 9.65 LRR134 pKa = 11.84 TTKK137 pKa = 10.75
Molecular weight: 17.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.5
IPC2_protein 11.345
IPC_protein 12.705
Toseland 12.866
ProMoST 13.364
Dawson 12.866
Bjellqvist 12.866
Wikipedia 13.349
Rodwell 12.53
Grimsley 12.91
Solomon 13.364
Lehninger 13.261
Nozaki 12.866
DTASelect 12.866
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.237
IPC_peptide 13.364
IPC2_peptide 12.354
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12026
0
12026
4669809
49
5616
388.3
43.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.657 ± 0.021
1.308 ± 0.011
5.16 ± 0.015
6.773 ± 0.031
3.422 ± 0.014
7.083 ± 0.025
2.417 ± 0.012
5.117 ± 0.017
5.415 ± 0.026
8.899 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.197 ± 0.009
3.7 ± 0.014
6.3 ± 0.027
3.946 ± 0.02
6.18 ± 0.021
8.023 ± 0.025
6.096 ± 0.02
6.16 ± 0.018
1.385 ± 0.009
2.763 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here