Streptococcus phage Javan155

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A1P7|A0A4D6A1P7_9CAUD HTH merR-type domain-containing protein OS=Streptococcus phage Javan155 OX=2548010 GN=Javan155_0045 PE=4 SV=1
MM1 pKa = 7.54TDD3 pKa = 3.63KK4 pKa = 10.88INPEE8 pKa = 3.96SMQVAFDD15 pKa = 3.93KK16 pKa = 11.1NYY18 pKa = 8.74QTFLAKK24 pKa = 10.52NADD27 pKa = 3.72YY28 pKa = 11.3GNSFEE33 pKa = 5.93KK34 pKa = 10.95SLDD37 pKa = 3.42DD38 pKa = 4.11LGIVAGVVRR47 pKa = 11.84IGDD50 pKa = 3.6KK51 pKa = 10.79YY52 pKa = 11.51NRR54 pKa = 11.84VCNLIKK60 pKa = 10.71NKK62 pKa = 10.29QNVSEE67 pKa = 4.45SLSDD71 pKa = 3.7TLNDD75 pKa = 3.42MANYY79 pKa = 10.6CMMLAVWLEE88 pKa = 3.93GQNDD92 pKa = 3.8DD93 pKa = 4.59

Molecular weight: 10.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A0B3|A0A4D6A0B3_9CAUD Uncharacterized protein OS=Streptococcus phage Javan155 OX=2548010 GN=Javan155_0032 PE=4 SV=1
MM1 pKa = 7.4NKK3 pKa = 10.22LEE5 pKa = 4.67LFLLVTTVALAIIARR20 pKa = 11.84VQYY23 pKa = 10.57EE24 pKa = 4.3VIKK27 pKa = 10.57KK28 pKa = 10.0HH29 pKa = 5.98NSPEE33 pKa = 3.71NKK35 pKa = 8.99RR36 pKa = 11.84RR37 pKa = 11.84IFRR40 pKa = 11.84EE41 pKa = 3.85VALEE45 pKa = 3.82NSKK48 pKa = 10.18GWSEE52 pKa = 3.73KK53 pKa = 9.44RR54 pKa = 11.84SRR56 pKa = 11.84GKK58 pKa = 10.31VVSS61 pKa = 3.7

Molecular weight: 7.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

10153

39

785

191.6

21.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.082 ± 0.606

0.601 ± 0.111

6.51 ± 0.284

7.249 ± 0.521

4.068 ± 0.231

6.619 ± 0.523

1.33 ± 0.16

7.023 ± 0.298

8.992 ± 0.555

8.185 ± 0.392

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.334 ± 0.214

5.555 ± 0.222

2.915 ± 0.269

3.871 ± 0.252

3.881 ± 0.289

6.432 ± 0.41

6.225 ± 0.308

6.264 ± 0.313

1.152 ± 0.123

3.713 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski