Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus; Oceanobacillus iheyensis

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3490 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8CXM1|Q8CXM1_OCEIH Leader peptide processing enzyme (Late competence protein) OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831) OX=221109 GN=OB0617 PE=3 SV=1
MM1 pKa = 7.25EE2 pKa = 5.37AANLFKK8 pKa = 10.83KK9 pKa = 10.54IFGFMLLLVLVGLLLGNIIQSNYY32 pKa = 9.36EE33 pKa = 4.26DD34 pKa = 3.55NHH36 pKa = 7.41SDD38 pKa = 2.95TDD40 pKa = 3.73YY41 pKa = 11.48HH42 pKa = 6.13VTGDD46 pKa = 3.93DD47 pKa = 3.51NMEE50 pKa = 4.53GGSIAPVDD58 pKa = 4.21SVGLEE63 pKa = 4.32PGDD66 pKa = 3.72KK67 pKa = 11.04APDD70 pKa = 3.69FEE72 pKa = 6.17LEE74 pKa = 4.18TLEE77 pKa = 4.61GEE79 pKa = 4.52SFRR82 pKa = 11.84LSDD85 pKa = 3.68YY86 pKa = 10.49QGKK89 pKa = 9.49KK90 pKa = 9.85VILNFWYY97 pKa = 8.38TWCPPCKK104 pKa = 10.02EE105 pKa = 4.72EE106 pKa = 3.96MPEE109 pKa = 3.86MQEE112 pKa = 4.9FYY114 pKa = 11.02DD115 pKa = 4.06DD116 pKa = 3.66YY117 pKa = 11.17KK118 pKa = 11.44EE119 pKa = 3.92EE120 pKa = 4.2VEE122 pKa = 4.15IVTVNMTEE130 pKa = 4.2YY131 pKa = 10.8EE132 pKa = 4.13KK133 pKa = 10.05TQQNVQDD140 pKa = 5.8FIDD143 pKa = 4.5EE144 pKa = 4.21YY145 pKa = 11.3DD146 pKa = 3.53FTFTVPLDD154 pKa = 3.82KK155 pKa = 11.15NSEE158 pKa = 4.0VSDD161 pKa = 4.34LYY163 pKa = 10.74TIYY166 pKa = 10.65AAPSTYY172 pKa = 10.4FIGTDD177 pKa = 4.01GIVQQEE183 pKa = 4.21RR184 pKa = 11.84KK185 pKa = 9.01IGPMDD190 pKa = 3.63YY191 pKa = 11.14EE192 pKa = 4.77FMQEE196 pKa = 3.8MVEE199 pKa = 4.52GINN202 pKa = 3.79

Molecular weight: 23.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8ELW8|Q8ELW8_OCEIH Hypothetical conserved protein OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831) OX=221109 GN=OB3096 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.08NGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3490

0

3490

1017601

24

2373

291.6

32.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.5 ± 0.045

0.605 ± 0.011

5.474 ± 0.04

7.66 ± 0.055

4.454 ± 0.036

6.696 ± 0.045

2.14 ± 0.019

8.533 ± 0.049

6.416 ± 0.042

9.418 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.812 ± 0.018

4.809 ± 0.031

3.509 ± 0.026

4.042 ± 0.031

3.761 ± 0.026

6.208 ± 0.028

5.494 ± 0.026

6.832 ± 0.036

1.014 ± 0.016

3.623 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski