Bacillus manliponensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus cereus group

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4374 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A073JVQ3|A0A073JVQ3_9BACI Glycerate kinase OS=Bacillus manliponensis OX=574376 GN=BAMA_05780 PE=3 SV=1
MM1 pKa = 7.97ADD3 pKa = 3.68FLDD6 pKa = 5.08PIQYY10 pKa = 7.69EE11 pKa = 4.34TGTAIQTFDD20 pKa = 3.62IIEE23 pKa = 4.5ANSSADD29 pKa = 3.4GYY31 pKa = 11.14EE32 pKa = 3.81VLLNIDD38 pKa = 4.02LDD40 pKa = 3.91SGYY43 pKa = 10.55EE44 pKa = 3.92LKK46 pKa = 10.64NVKK49 pKa = 10.38LNITADD55 pKa = 3.38EE56 pKa = 4.2LLAYY60 pKa = 7.28EE61 pKa = 4.33TTDD64 pKa = 2.4IHH66 pKa = 6.45EE67 pKa = 4.36AVKK70 pKa = 10.12YY71 pKa = 10.55ALGFFHH77 pKa = 7.16QSS79 pKa = 2.59

Molecular weight: 8.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A073K5K4|A0A073K5K4_9BACI Integrase OS=Bacillus manliponensis OX=574376 GN=BAMA_12330 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4374

0

4374

1221822

30

2723

279.3

31.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.776 ± 0.036

0.875 ± 0.011

4.659 ± 0.028

7.848 ± 0.054

4.667 ± 0.035

6.628 ± 0.042

2.255 ± 0.019

7.889 ± 0.044

7.148 ± 0.042

9.591 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.924 ± 0.017

4.326 ± 0.026

3.326 ± 0.019

3.8 ± 0.025

3.913 ± 0.028

5.686 ± 0.026

5.522 ± 0.024

7.362 ± 0.03

1.021 ± 0.015

3.786 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski