Avon-Heathcote Estuary associated circular virus 26

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IB96|A0A0C5IB96_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 26 OX=1618250 PE=4 SV=1
MM1 pKa = 8.04PSRR4 pKa = 11.84QGVYY8 pKa = 9.87FIITVPYY15 pKa = 9.5EE16 pKa = 4.15KK17 pKa = 10.65YY18 pKa = 9.72EE19 pKa = 4.2KK20 pKa = 10.24PIEE23 pKa = 4.02LPRR26 pKa = 11.84GFRR29 pKa = 11.84YY30 pKa = 9.86ISGQGEE36 pKa = 4.05IGASGYY42 pKa = 8.92HH43 pKa = 5.97HH44 pKa = 6.61WQIICAFEE52 pKa = 3.96QKK54 pKa = 8.91KK55 pKa = 7.49TLTGAKK61 pKa = 9.84SYY63 pKa = 8.62FAPEE67 pKa = 3.38AHH69 pKa = 7.22LEE71 pKa = 4.2FTRR74 pKa = 11.84SRR76 pKa = 11.84CARR79 pKa = 11.84DD80 pKa = 3.41YY81 pKa = 10.76CHH83 pKa = 7.17KK84 pKa = 10.45EE85 pKa = 3.62EE86 pKa = 4.15TRR88 pKa = 11.84IPDD91 pKa = 3.11SSFEE95 pKa = 4.03FGTYY99 pKa = 6.87PTRR102 pKa = 11.84VNNKK106 pKa = 8.8QDD108 pKa = 3.0WDD110 pKa = 3.99LVLEE114 pKa = 4.31KK115 pKa = 10.64AKK117 pKa = 10.54KK118 pKa = 10.06GQFDD122 pKa = 5.51LIDD125 pKa = 4.46GDD127 pKa = 3.95VMMRR131 pKa = 11.84HH132 pKa = 5.95YY133 pKa = 11.66NNIRR137 pKa = 11.84KK138 pKa = 9.42IYY140 pKa = 10.54SDD142 pKa = 3.27FQKK145 pKa = 10.25PVKK148 pKa = 10.53RR149 pKa = 11.84EE150 pKa = 3.81VQVVNVYY157 pKa = 8.78WGQTGTGKK165 pKa = 8.65TKK167 pKa = 10.75SVFEE171 pKa = 4.49EE172 pKa = 4.12IGDD175 pKa = 3.79DD176 pKa = 4.4FYY178 pKa = 12.2VKK180 pKa = 10.07MPSTKK185 pKa = 9.74WFDD188 pKa = 3.49GYY190 pKa = 10.66RR191 pKa = 11.84GQEE194 pKa = 3.57NVVIDD199 pKa = 4.35EE200 pKa = 4.3FTGQVDD206 pKa = 3.39ITHH209 pKa = 7.62LLRR212 pKa = 11.84WLDD215 pKa = 3.62QYY217 pKa = 11.47PCTVEE222 pKa = 4.5VKK224 pKa = 10.54GSQVFLNTKK233 pKa = 9.37KK234 pKa = 9.22WWITSNIDD242 pKa = 3.1PAKK245 pKa = 9.17WYY247 pKa = 9.39EE248 pKa = 4.23GKK250 pKa = 10.33DD251 pKa = 3.41EE252 pKa = 4.24EE253 pKa = 5.19QIKK256 pKa = 9.95ALKK259 pKa = 10.24RR260 pKa = 11.84RR261 pKa = 11.84FTNVVHH267 pKa = 5.75YY268 pKa = 8.68LKK270 pKa = 10.42PFKK273 pKa = 10.51KK274 pKa = 10.45

Molecular weight: 32.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IB96|A0A0C5IB96_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 26 OX=1618250 PE=4 SV=1
MM1 pKa = 8.14DD2 pKa = 3.68KK3 pKa = 10.66QIYY6 pKa = 10.55DD7 pKa = 3.45MAAKK11 pKa = 9.97YY12 pKa = 10.0IKK14 pKa = 10.52SNLGVHH20 pKa = 5.42VPSYY24 pKa = 8.69NTTRR28 pKa = 11.84TVYY31 pKa = 8.81NTYY34 pKa = 10.51RR35 pKa = 11.84NARR38 pKa = 11.84RR39 pKa = 11.84SMKK42 pKa = 9.49STNQKK47 pKa = 9.06MKK49 pKa = 10.67SSSSKK54 pKa = 8.61NWKK57 pKa = 8.3VSPRR61 pKa = 11.84ITYY64 pKa = 6.58PTSMSSTKK72 pKa = 10.0TAVLPPKK79 pKa = 10.37KK80 pKa = 9.78KK81 pKa = 9.15FRR83 pKa = 11.84KK84 pKa = 8.55IRR86 pKa = 11.84RR87 pKa = 11.84TIVAGSRR94 pKa = 11.84KK95 pKa = 9.36SVPKK99 pKa = 10.3KK100 pKa = 8.16VRR102 pKa = 11.84KK103 pKa = 8.79YY104 pKa = 10.39VKK106 pKa = 10.18KK107 pKa = 10.63AVNDD111 pKa = 3.55KK112 pKa = 9.98TKK114 pKa = 9.26YY115 pKa = 7.31TQGPGARR122 pKa = 11.84IEE124 pKa = 4.52LYY126 pKa = 9.31NTFHH130 pKa = 7.55LDD132 pKa = 2.88TGNSVIQTGQFGSSPGDD149 pKa = 3.58SNHH152 pKa = 6.35AFIPFFTDD160 pKa = 2.66TVMNTEE166 pKa = 3.98FRR168 pKa = 11.84IGNNPVGDD176 pKa = 4.15DD177 pKa = 4.45AGMLDD182 pKa = 4.58PPPAFNASSGASVQYY197 pKa = 10.77IKK199 pKa = 10.93DD200 pKa = 3.63NEE202 pKa = 4.79SIVIDD207 pKa = 4.15HH208 pKa = 6.32YY209 pKa = 11.54SATFFMTNTSDD220 pKa = 3.37TKK222 pKa = 11.6AMFHH226 pKa = 5.41IQEE229 pKa = 4.37WVCNRR234 pKa = 11.84DD235 pKa = 2.75SDD237 pKa = 4.24VNIVVRR243 pKa = 11.84TTEE246 pKa = 3.71QFNNQTWRR254 pKa = 11.84NDD256 pKa = 3.38PGFATIVPVSSTNLFKK272 pKa = 11.01QPNFEE277 pKa = 3.88ISKK280 pKa = 10.3VPGISQYY287 pKa = 8.42WKK289 pKa = 9.24KK290 pKa = 11.2GSIKK294 pKa = 10.31RR295 pKa = 11.84QFEE298 pKa = 4.38LMPGEE303 pKa = 4.6SIEE306 pKa = 4.52LSLPFGKK313 pKa = 10.33KK314 pKa = 7.52YY315 pKa = 10.28WNLQRR320 pKa = 11.84FNDD323 pKa = 3.75QNEE326 pKa = 4.34TLTGRR331 pKa = 11.84FEE333 pKa = 4.0YY334 pKa = 10.45HH335 pKa = 6.68KK336 pKa = 10.77NLSRR340 pKa = 11.84YY341 pKa = 8.62LQVSVRR347 pKa = 11.84GSVGFSADD355 pKa = 3.84TPPKK359 pKa = 10.65SSFQGTNVYY368 pKa = 10.7YY369 pKa = 10.55KK370 pKa = 10.55VLKK373 pKa = 9.98TMIAHH378 pKa = 6.14RR379 pKa = 11.84VDD381 pKa = 4.75RR382 pKa = 11.84FGQQPKK388 pKa = 9.74RR389 pKa = 11.84YY390 pKa = 8.84RR391 pKa = 11.84IIGNPSEE398 pKa = 4.43VVSSFNGTAVDD409 pKa = 4.36LGRR412 pKa = 11.84PAITEE417 pKa = 3.95PVEE420 pKa = 5.34SIIQTLQASTT430 pKa = 3.59

Molecular weight: 48.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

704

274

430

352.0

40.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.119 ± 0.511

0.71 ± 0.459

4.972 ± 0.531

4.83 ± 1.511

5.54 ± 0.183

6.25 ± 0.419

1.989 ± 0.346

5.966 ± 0.146

8.807 ± 0.641

3.977 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.131 ± 0.41

5.54 ± 1.38

5.256 ± 0.536

4.83 ± 0.171

5.682 ± 0.127

7.813 ± 2.101

7.528 ± 0.81

7.386 ± 0.053

1.847 ± 0.657

4.83 ± 0.618
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski