Amino acid dipepetide frequency for Acetobacter sp. DsW_54

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.033AlaAla: 15.033 ± 0.223
1.534AlaCys: 1.534 ± 0.052
6.254AlaAsp: 6.254 ± 0.088
6.705AlaGlu: 6.705 ± 0.128
3.946AlaPhe: 3.946 ± 0.082
10.569AlaGly: 10.569 ± 0.134
2.882AlaHis: 2.882 ± 0.07
5.278AlaIle: 5.278 ± 0.097
3.116AlaLys: 3.116 ± 0.076
13.715AlaLeu: 13.715 ± 0.217
3.46AlaMet: 3.46 ± 0.062
2.718AlaAsn: 2.718 ± 0.062
6.26AlaPro: 6.26 ± 0.116
5.683AlaGln: 5.683 ± 0.117
8.54AlaArg: 8.54 ± 0.13
6.552AlaSer: 6.552 ± 0.112
6.527AlaThr: 6.527 ± 0.105
8.084AlaVal: 8.084 ± 0.116
1.599AlaTrp: 1.599 ± 0.054
2.404AlaTyr: 2.404 ± 0.065
0.0AlaXaa: 0.0 ± 0.0
Cys
1.379CysAla: 1.379 ± 0.047
0.189CysCys: 0.189 ± 0.016
0.518CysAsp: 0.518 ± 0.028
0.438CysGlu: 0.438 ± 0.022
0.377CysPhe: 0.377 ± 0.026
1.18CysGly: 1.18 ± 0.037
0.298CysHis: 0.298 ± 0.022
0.524CysIle: 0.524 ± 0.028
0.242CysLys: 0.242 ± 0.017
1.105CysLeu: 1.105 ± 0.041
0.264CysMet: 0.264 ± 0.021
0.256CysAsn: 0.256 ± 0.02
0.615CysPro: 0.615 ± 0.032
0.333CysGln: 0.333 ± 0.024
0.685CysArg: 0.685 ± 0.029
0.616CysSer: 0.616 ± 0.036
0.605CysThr: 0.605 ± 0.028
0.928CysVal: 0.928 ± 0.041
0.121CysTrp: 0.121 ± 0.013
0.201CysTyr: 0.201 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
6.247AspAla: 6.247 ± 0.104
0.497AspCys: 0.497 ± 0.027
2.479AspAsp: 2.479 ± 0.072
2.708AspGlu: 2.708 ± 0.063
1.841AspPhe: 1.841 ± 0.052
4.405AspGly: 4.405 ± 0.085
1.15AspHis: 1.15 ± 0.039
2.982AspIle: 2.982 ± 0.073
1.584AspLys: 1.584 ± 0.061
5.392AspLeu: 5.392 ± 0.091
1.729AspMet: 1.729 ± 0.05
1.461AspAsn: 1.461 ± 0.046
2.9AspPro: 2.9 ± 0.082
1.725AspGln: 1.725 ± 0.046
3.304AspArg: 3.304 ± 0.069
2.193AspSer: 2.193 ± 0.063
2.83AspThr: 2.83 ± 0.061
4.355AspVal: 4.355 ± 0.084
0.845AspTrp: 0.845 ± 0.04
1.387AspTyr: 1.387 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
6.499GluAla: 6.499 ± 0.097
0.411GluCys: 0.411 ± 0.029
2.615GluAsp: 2.615 ± 0.072
3.114GluGlu: 3.114 ± 0.092
1.434GluPhe: 1.434 ± 0.052
4.035GluGly: 4.035 ± 0.077
1.52GluHis: 1.52 ± 0.058
2.525GluIle: 2.525 ± 0.067
2.177GluLys: 2.177 ± 0.064
4.721GluLeu: 4.721 ± 0.096
1.229GluMet: 1.229 ± 0.048
1.622GluAsn: 1.622 ± 0.053
2.135GluPro: 2.135 ± 0.058
2.929GluGln: 2.929 ± 0.076
4.11GluArg: 4.11 ± 0.092
2.232GluSer: 2.232 ± 0.057
2.858GluThr: 2.858 ± 0.069
3.376GluVal: 3.376 ± 0.08
0.607GluTrp: 0.607 ± 0.031
0.942GluTyr: 0.942 ± 0.035
0.0GluXaa: 0.0 ± 0.0
Phe
4.208PheAla: 4.208 ± 0.081
0.514PheCys: 0.514 ± 0.028
2.01PheAsp: 2.01 ± 0.058
1.709PheGlu: 1.709 ± 0.05
1.387PhePhe: 1.387 ± 0.049
3.439PheGly: 3.439 ± 0.068
0.746PheHis: 0.746 ± 0.036
1.551PheIle: 1.551 ± 0.045
0.894PheLys: 0.894 ± 0.038
3.402PheLeu: 3.402 ± 0.074
0.995PheMet: 0.995 ± 0.042
1.058PheAsn: 1.058 ± 0.043
1.543PhePro: 1.543 ± 0.05
0.903PheGln: 0.903 ± 0.039
2.039PheArg: 2.039 ± 0.048
2.318PheSer: 2.318 ± 0.059
2.017PheThr: 2.017 ± 0.05
2.803PheVal: 2.803 ± 0.067
0.523PheTrp: 0.523 ± 0.027
0.915PheTyr: 0.915 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
8.646GlyAla: 8.646 ± 0.132
1.067GlyCys: 1.067 ± 0.047
3.571GlyAsp: 3.571 ± 0.068
3.85GlyGlu: 3.85 ± 0.078
3.258GlyPhe: 3.258 ± 0.082
7.3GlyGly: 7.3 ± 0.151
2.2GlyHis: 2.2 ± 0.059
4.19GlyIle: 4.19 ± 0.076
3.059GlyLys: 3.059 ± 0.072
9.145GlyLeu: 9.145 ± 0.118
2.616GlyMet: 2.616 ± 0.056
2.27GlyAsn: 2.27 ± 0.067
3.45GlyPro: 3.45 ± 0.071
3.713GlyGln: 3.713 ± 0.059
5.563GlyArg: 5.563 ± 0.091
4.519GlySer: 4.519 ± 0.101
5.025GlyThr: 5.025 ± 0.127
6.717GlyVal: 6.717 ± 0.106
1.375GlyTrp: 1.375 ± 0.042
2.139GlyTyr: 2.139 ± 0.064
0.0GlyXaa: 0.0 ± 0.0
His
3.022HisAla: 3.022 ± 0.077
0.266HisCys: 0.266 ± 0.022
1.428HisAsp: 1.428 ± 0.045
1.182HisGlu: 1.182 ± 0.04
0.965HisPhe: 0.965 ± 0.038
2.125HisGly: 2.125 ± 0.054
0.712HisHis: 0.712 ± 0.037
1.212HisIle: 1.212 ± 0.046
0.78HisLys: 0.78 ± 0.034
2.266HisLeu: 2.266 ± 0.065
0.678HisMet: 0.678 ± 0.031
0.836HisAsn: 0.836 ± 0.033
1.547HisPro: 1.547 ± 0.052
0.702HisGln: 0.702 ± 0.029
1.41HisArg: 1.41 ± 0.046
1.122HisSer: 1.122 ± 0.038
1.506HisThr: 1.506 ± 0.045
1.859HisVal: 1.859 ± 0.051
0.388HisTrp: 0.388 ± 0.023
0.674HisTyr: 0.674 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
6.157IleAla: 6.157 ± 0.087
0.552IleCys: 0.552 ± 0.027
2.502IleAsp: 2.502 ± 0.067
2.461IleGlu: 2.461 ± 0.068
1.497IlePhe: 1.497 ± 0.056
4.309IleGly: 4.309 ± 0.079
0.975IleHis: 0.975 ± 0.035
2.174IleIle: 2.174 ± 0.063
1.359IleLys: 1.359 ± 0.046
4.375IleLeu: 4.375 ± 0.081
1.174IleMet: 1.174 ± 0.043
1.499IleAsn: 1.499 ± 0.054
2.509IlePro: 2.509 ± 0.064
1.425IleGln: 1.425 ± 0.049
3.035IleArg: 3.035 ± 0.066
2.806IleSer: 2.806 ± 0.07
3.095IleThr: 3.095 ± 0.07
3.593IleVal: 3.593 ± 0.064
0.559IleTrp: 0.559 ± 0.03
1.004IleTyr: 1.004 ± 0.041
0.0IleXaa: 0.0 ± 0.0
Lys
3.83LysAla: 3.83 ± 0.087
0.151LysCys: 0.151 ± 0.016
1.756LysAsp: 1.756 ± 0.058
1.473LysGlu: 1.473 ± 0.053
0.759LysPhe: 0.759 ± 0.032
2.554LysGly: 2.554 ± 0.074
0.595LysHis: 0.595 ± 0.031
1.627LysIle: 1.627 ± 0.051
1.428LysLys: 1.428 ± 0.052
3.078LysLeu: 3.078 ± 0.08
0.747LysMet: 0.747 ± 0.032
1.065LysAsn: 1.065 ± 0.045
1.859LysPro: 1.859 ± 0.057
1.127LysGln: 1.127 ± 0.046
1.928LysArg: 1.928 ± 0.053
1.557LysSer: 1.557 ± 0.057
2.024LysThr: 2.024 ± 0.055
2.178LysVal: 2.178 ± 0.069
0.321LysTrp: 0.321 ± 0.023
0.561LysTyr: 0.561 ± 0.032
0.0LysXaa: 0.0 ± 0.0
Leu
13.475LeuAla: 13.475 ± 0.195
1.37LeuCys: 1.37 ± 0.05
5.908LeuAsp: 5.908 ± 0.095
5.131LeuGlu: 5.131 ± 0.096
3.793LeuPhe: 3.793 ± 0.097
7.969LeuGly: 7.969 ± 0.11
2.745LeuHis: 2.745 ± 0.067
4.172LeuIle: 4.172 ± 0.08
3.111LeuLys: 3.111 ± 0.073
10.499LeuLeu: 10.499 ± 0.185
2.465LeuMet: 2.465 ± 0.064
2.923LeuAsn: 2.923 ± 0.066
6.832LeuPro: 6.832 ± 0.126
3.53LeuGln: 3.53 ± 0.07
7.307LeuArg: 7.307 ± 0.109
6.866LeuSer: 6.866 ± 0.115
5.6LeuThr: 5.6 ± 0.1
7.578LeuVal: 7.578 ± 0.115
1.222LeuTrp: 1.222 ± 0.041
2.125LeuTyr: 2.125 ± 0.058
0.0LeuXaa: 0.0 ± 0.0
Met
3.405MetAla: 3.405 ± 0.072
0.161MetCys: 0.161 ± 0.015
1.37MetAsp: 1.37 ± 0.043
1.377MetGlu: 1.377 ± 0.041
0.661MetPhe: 0.661 ± 0.034
2.188MetGly: 2.188 ± 0.056
0.571MetHis: 0.571 ± 0.028
1.105MetIle: 1.105 ± 0.043
0.812MetLys: 0.812 ± 0.035
2.857MetLeu: 2.857 ± 0.071
0.606MetMet: 0.606 ± 0.034
0.785MetAsn: 0.785 ± 0.04
1.721MetPro: 1.721 ± 0.052
1.171MetGln: 1.171 ± 0.045
1.769MetArg: 1.769 ± 0.051
1.725MetSer: 1.725 ± 0.045
1.846MetThr: 1.846 ± 0.048
1.942MetVal: 1.942 ± 0.051
0.232MetTrp: 0.232 ± 0.018
0.302MetTyr: 0.302 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
3.563AsnAla: 3.563 ± 0.074
0.263AsnCys: 0.263 ± 0.021
1.232AsnAsp: 1.232 ± 0.041
1.178AsnGlu: 1.178 ± 0.045
0.9AsnPhe: 0.9 ± 0.036
2.721AsnGly: 2.721 ± 0.071
0.644AsnHis: 0.644 ± 0.032
1.529AsnIle: 1.529 ± 0.043
0.856AsnLys: 0.856 ± 0.04
2.643AsnLeu: 2.643 ± 0.064
0.747AsnMet: 0.747 ± 0.037
0.944AsnAsn: 0.944 ± 0.05
1.947AsnPro: 1.947 ± 0.053
0.879AsnGln: 0.879 ± 0.039
1.769AsnArg: 1.769 ± 0.044
1.343AsnSer: 1.343 ± 0.055
1.921AsnThr: 1.921 ± 0.055
2.108AsnVal: 2.108 ± 0.056
0.387AsnTrp: 0.387 ± 0.026
0.665AsnTyr: 0.665 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
6.934ProAla: 6.934 ± 0.13
0.475ProCys: 0.475 ± 0.028
3.981ProAsp: 3.981 ± 0.077
3.765ProGlu: 3.765 ± 0.068
2.023ProPhe: 2.023 ± 0.057
4.401ProGly: 4.401 ± 0.078
1.519ProHis: 1.519 ± 0.05
1.969ProIle: 1.969 ± 0.046
1.386ProLys: 1.386 ± 0.048
5.213ProLeu: 5.213 ± 0.091
1.259ProMet: 1.259 ± 0.044
1.355ProAsn: 1.355 ± 0.048
3.248ProPro: 3.248 ± 0.095
2.372ProGln: 2.372 ± 0.063
2.842ProArg: 2.842 ± 0.075
2.93ProSer: 2.93 ± 0.077
3.009ProThr: 3.009 ± 0.066
4.795ProVal: 4.795 ± 0.082
0.747ProTrp: 0.747 ± 0.028
1.242ProTyr: 1.242 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
5.747GlnAla: 5.747 ± 0.133
0.297GlnCys: 0.297 ± 0.021
2.074GlnAsp: 2.074 ± 0.052
1.887GlnGlu: 1.887 ± 0.056
1.143GlnPhe: 1.143 ± 0.043
2.83GlnGly: 2.83 ± 0.066
0.969GlnHis: 0.969 ± 0.043
2.176GlnIle: 2.176 ± 0.046
1.5GlnLys: 1.5 ± 0.05
3.198GlnLeu: 3.198 ± 0.07
1.027GlnMet: 1.027 ± 0.037
1.252GlnAsn: 1.252 ± 0.044
2.358GlnPro: 2.358 ± 0.062
1.804GlnGln: 1.804 ± 0.058
2.385GlnArg: 2.385 ± 0.057
2.178GlnSer: 2.178 ± 0.066
2.717GlnThr: 2.717 ± 0.076
2.69GlnVal: 2.69 ± 0.068
0.469GlnTrp: 0.469 ± 0.029
0.802GlnTyr: 0.802 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
7.127ArgAla: 7.127 ± 0.114
0.581ArgCys: 0.581 ± 0.03
3.157ArgAsp: 3.157 ± 0.07
3.474ArgGlu: 3.474 ± 0.069
2.587ArgPhe: 2.587 ± 0.061
4.265ArgGly: 4.265 ± 0.088
1.863ArgHis: 1.863 ± 0.053
3.665ArgIle: 3.665 ± 0.07
2.387ArgLys: 2.387 ± 0.065
7.671ArgLeu: 7.671 ± 0.137
1.825ArgMet: 1.825 ± 0.056
2.058ArgAsn: 2.058 ± 0.053
3.296ArgPro: 3.296 ± 0.069
2.919ArgGln: 2.919 ± 0.065
4.788ArgArg: 4.788 ± 0.104
3.419ArgSer: 3.419 ± 0.066
3.611ArgThr: 3.611 ± 0.069
4.686ArgVal: 4.686 ± 0.068
0.896ArgTrp: 0.896 ± 0.04
1.698ArgTyr: 1.698 ± 0.057
0.0ArgXaa: 0.0 ± 0.0
Ser
6.46SerAla: 6.46 ± 0.108
0.565SerCys: 0.565 ± 0.029
2.541SerAsp: 2.541 ± 0.059
2.14SerGlu: 2.14 ± 0.055
2.431SerPhe: 2.431 ± 0.065
5.406SerGly: 5.406 ± 0.114
1.215SerHis: 1.215 ± 0.043
2.765SerIle: 2.765 ± 0.069
1.387SerLys: 1.387 ± 0.044
5.94SerLeu: 5.94 ± 0.099
1.505SerMet: 1.505 ± 0.045
1.485SerAsn: 1.485 ± 0.053
3.064SerPro: 3.064 ± 0.062
1.64SerGln: 1.64 ± 0.055
3.369SerArg: 3.369 ± 0.071
3.378SerSer: 3.378 ± 0.1
3.222SerThr: 3.222 ± 0.081
4.514SerVal: 4.514 ± 0.085
0.732SerTrp: 0.732 ± 0.033
1.356SerTyr: 1.356 ± 0.049
0.0SerXaa: 0.0 ± 0.0
Thr
6.497ThrAla: 6.497 ± 0.107
0.603ThrCys: 0.603 ± 0.031
2.858ThrAsp: 2.858 ± 0.057
2.59ThrGlu: 2.59 ± 0.069
1.958ThrPhe: 1.958 ± 0.053
5.33ThrGly: 5.33 ± 0.086
1.488ThrHis: 1.488 ± 0.05
2.707ThrIle: 2.707 ± 0.065
1.348ThrLys: 1.348 ± 0.042
7.1ThrLeu: 7.1 ± 0.107
1.235ThrMet: 1.235 ± 0.039
1.502ThrAsn: 1.502 ± 0.063
4.23ThrPro: 4.23 ± 0.089
2.279ThrGln: 2.279 ± 0.054
3.529ThrArg: 3.529 ± 0.067
3.119ThrSer: 3.119 ± 0.096
3.537ThrThr: 3.537 ± 0.088
4.37ThrVal: 4.37 ± 0.077
0.711ThrTrp: 0.711 ± 0.033
1.267ThrTyr: 1.267 ± 0.049
0.0ThrXaa: 0.0 ± 0.0
Val
8.947ValAla: 8.947 ± 0.14
0.914ValCys: 0.914 ± 0.036
3.793ValAsp: 3.793 ± 0.076
4.276ValGlu: 4.276 ± 0.084
2.763ValPhe: 2.763 ± 0.064
5.549ValGly: 5.549 ± 0.1
1.663ValHis: 1.663 ± 0.051
3.446ValIle: 3.446 ± 0.091
2.022ValLys: 2.022 ± 0.06
8.514ValLeu: 8.514 ± 0.121
2.164ValMet: 2.164 ± 0.055
1.902ValAsn: 1.902 ± 0.059
4.107ValPro: 4.107 ± 0.082
2.799ValGln: 2.799 ± 0.068
4.985ValArg: 4.985 ± 0.097
4.348ValSer: 4.348 ± 0.073
4.343ValThr: 4.343 ± 0.088
6.093ValVal: 6.093 ± 0.11
1.041ValTrp: 1.041 ± 0.036
1.432ValTyr: 1.432 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
1.132TrpAla: 1.132 ± 0.041
0.194TrpCys: 0.194 ± 0.015
0.545TrpAsp: 0.545 ± 0.032
0.53TrpGlu: 0.53 ± 0.028
0.48TrpPhe: 0.48 ± 0.026
0.978TrpGly: 0.978 ± 0.043
0.437TrpHis: 0.437 ± 0.025
0.551TrpIle: 0.551 ± 0.027
0.506TrpLys: 0.506 ± 0.023
1.7TrpLeu: 1.7 ± 0.045
0.37TrpMet: 0.37 ± 0.023
0.476TrpAsn: 0.476 ± 0.032
0.732TrpPro: 0.732 ± 0.035
0.736TrpGln: 0.736 ± 0.034
1.064TrpArg: 1.064 ± 0.039
0.752TrpSer: 0.752 ± 0.034
0.691TrpThr: 0.691 ± 0.035
0.903TrpVal: 0.903 ± 0.039
0.184TrpTrp: 0.184 ± 0.017
0.291TrpTyr: 0.291 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.553TyrAla: 2.553 ± 0.057
0.27TyrCys: 0.27 ± 0.02
1.42TyrAsp: 1.42 ± 0.045
1.101TyrGlu: 1.101 ± 0.038
0.771TyrPhe: 0.771 ± 0.035
2.12TyrGly: 2.12 ± 0.056
0.49TyrHis: 0.49 ± 0.03
0.914TyrIle: 0.914 ± 0.036
0.667TyrLys: 0.667 ± 0.028
2.085TyrLeu: 2.085 ± 0.065
0.51TyrMet: 0.51 ± 0.025
0.773TyrAsn: 0.773 ± 0.041
1.115TyrPro: 1.115 ± 0.043
0.834TyrGln: 0.834 ± 0.038
1.553TyrArg: 1.553 ± 0.054
1.144TyrSer: 1.144 ± 0.043
1.271TyrThr: 1.271 ± 0.052
1.53TyrVal: 1.53 ± 0.048
0.29TyrTrp: 0.29 ± 0.021
0.617TyrTyr: 0.617 ± 0.033
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2200 proteins (707855 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski