Amino acid dipepetide frequency for Klebsiella phage KPN N54

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.382AlaAla: 12.382 ± 1.27
0.931AlaCys: 0.931 ± 0.314
6.191AlaAsp: 6.191 ± 0.453
7.506AlaGlu: 7.506 ± 0.779
3.78AlaPhe: 3.78 ± 0.521
8.054AlaGly: 8.054 ± 0.588
1.26AlaHis: 1.26 ± 0.22
5.534AlaIle: 5.534 ± 0.572
6.41AlaLys: 6.41 ± 0.862
7.67AlaLeu: 7.67 ± 0.605
3.068AlaMet: 3.068 ± 0.465
3.835AlaAsn: 3.835 ± 0.435
3.506AlaPro: 3.506 ± 0.422
4.876AlaGln: 4.876 ± 0.626
4.986AlaArg: 4.986 ± 0.573
4.876AlaSer: 4.876 ± 0.566
5.698AlaThr: 5.698 ± 0.682
7.177AlaVal: 7.177 ± 0.867
1.37AlaTrp: 1.37 ± 0.333
3.78AlaTyr: 3.78 ± 0.403
0.0AlaXaa: 0.0 ± 0.0
Cys
1.041CysAla: 1.041 ± 0.209
0.274CysCys: 0.274 ± 0.117
0.712CysAsp: 0.712 ± 0.251
0.603CysGlu: 0.603 ± 0.195
0.548CysPhe: 0.548 ± 0.134
0.877CysGly: 0.877 ± 0.219
0.219CysHis: 0.219 ± 0.093
0.603CysIle: 0.603 ± 0.169
0.384CysLys: 0.384 ± 0.155
0.548CysLeu: 0.548 ± 0.183
0.164CysMet: 0.164 ± 0.097
0.438CysAsn: 0.438 ± 0.157
0.603CysPro: 0.603 ± 0.248
0.164CysGln: 0.164 ± 0.093
1.041CysArg: 1.041 ± 0.293
0.548CysSer: 0.548 ± 0.2
0.603CysThr: 0.603 ± 0.179
0.438CysVal: 0.438 ± 0.163
0.11CysTrp: 0.11 ± 0.069
0.164CysTyr: 0.164 ± 0.099
0.0CysXaa: 0.0 ± 0.0
Asp
7.068AspAla: 7.068 ± 0.544
0.493AspCys: 0.493 ± 0.171
5.534AspAsp: 5.534 ± 0.653
5.095AspGlu: 5.095 ± 0.591
2.575AspPhe: 2.575 ± 0.33
5.26AspGly: 5.26 ± 0.566
0.712AspHis: 0.712 ± 0.184
4.0AspIle: 4.0 ± 0.513
2.794AspLys: 2.794 ± 0.386
4.821AspLeu: 4.821 ± 0.599
1.918AspMet: 1.918 ± 0.3
2.192AspAsn: 2.192 ± 0.333
3.123AspPro: 3.123 ± 0.388
1.972AspGln: 1.972 ± 0.234
2.849AspArg: 2.849 ± 0.365
3.013AspSer: 3.013 ± 0.449
3.945AspThr: 3.945 ± 0.385
4.328AspVal: 4.328 ± 0.449
1.918AspTrp: 1.918 ± 0.368
1.972AspTyr: 1.972 ± 0.354
0.0AspXaa: 0.0 ± 0.0
Glu
5.369GluAla: 5.369 ± 0.472
0.493GluCys: 0.493 ± 0.195
3.945GluAsp: 3.945 ± 0.494
4.821GluGlu: 4.821 ± 0.66
2.192GluPhe: 2.192 ± 0.393
4.328GluGly: 4.328 ± 0.517
0.712GluHis: 0.712 ± 0.203
3.726GluIle: 3.726 ± 0.451
3.452GluLys: 3.452 ± 0.63
5.205GluLeu: 5.205 ± 0.542
2.246GluMet: 2.246 ± 0.425
2.575GluAsn: 2.575 ± 0.409
1.863GluPro: 1.863 ± 0.353
2.192GluGln: 2.192 ± 0.432
3.616GluArg: 3.616 ± 0.461
3.123GluSer: 3.123 ± 0.397
2.411GluThr: 2.411 ± 0.383
4.602GluVal: 4.602 ± 0.549
1.26GluTrp: 1.26 ± 0.22
2.301GluTyr: 2.301 ± 0.321
0.0GluXaa: 0.0 ± 0.0
Phe
3.835PheAla: 3.835 ± 0.521
0.384PheCys: 0.384 ± 0.157
2.356PheAsp: 2.356 ± 0.337
1.972PheGlu: 1.972 ± 0.297
1.26PhePhe: 1.26 ± 0.331
2.849PheGly: 2.849 ± 0.404
0.438PheHis: 0.438 ± 0.149
2.411PheIle: 2.411 ± 0.296
1.644PheLys: 1.644 ± 0.418
2.52PheLeu: 2.52 ± 0.418
1.041PheMet: 1.041 ± 0.235
1.644PheAsn: 1.644 ± 0.333
1.644PhePro: 1.644 ± 0.265
1.096PheGln: 1.096 ± 0.212
2.465PheArg: 2.465 ± 0.339
2.246PheSer: 2.246 ± 0.317
2.246PheThr: 2.246 ± 0.438
1.918PheVal: 1.918 ± 0.31
0.603PheTrp: 0.603 ± 0.189
0.877PheTyr: 0.877 ± 0.202
0.0PheXaa: 0.0 ± 0.0
Gly
6.903GlyAla: 6.903 ± 0.79
0.986GlyCys: 0.986 ± 0.285
4.547GlyAsp: 4.547 ± 0.595
4.383GlyGlu: 4.383 ± 0.525
2.082GlyPhe: 2.082 ± 0.378
5.095GlyGly: 5.095 ± 0.645
1.26GlyHis: 1.26 ± 0.282
4.219GlyIle: 4.219 ± 0.36
4.438GlyLys: 4.438 ± 0.57
6.794GlyLeu: 6.794 ± 0.824
1.972GlyMet: 1.972 ± 0.339
2.685GlyAsn: 2.685 ± 0.435
2.192GlyPro: 2.192 ± 0.32
2.192GlyGln: 2.192 ± 0.325
5.643GlyArg: 5.643 ± 0.599
4.274GlySer: 4.274 ± 0.637
4.821GlyThr: 4.821 ± 0.802
5.369GlyVal: 5.369 ± 0.526
1.918GlyTrp: 1.918 ± 0.327
3.342GlyTyr: 3.342 ± 0.485
0.0GlyXaa: 0.0 ± 0.0
His
1.26HisAla: 1.26 ± 0.259
0.274HisCys: 0.274 ± 0.118
0.931HisAsp: 0.931 ± 0.221
0.767HisGlu: 0.767 ± 0.185
0.493HisPhe: 0.493 ± 0.155
1.315HisGly: 1.315 ± 0.33
0.11HisHis: 0.11 ± 0.091
1.096HisIle: 1.096 ± 0.23
0.986HisLys: 0.986 ± 0.225
1.26HisLeu: 1.26 ± 0.32
0.438HisMet: 0.438 ± 0.169
0.603HisAsn: 0.603 ± 0.163
0.712HisPro: 0.712 ± 0.213
0.329HisGln: 0.329 ± 0.13
1.205HisArg: 1.205 ± 0.279
0.822HisSer: 0.822 ± 0.193
0.931HisThr: 0.931 ± 0.237
0.767HisVal: 0.767 ± 0.216
0.219HisTrp: 0.219 ± 0.104
0.822HisTyr: 0.822 ± 0.226
0.0HisXaa: 0.0 ± 0.0
Ile
5.369IleAla: 5.369 ± 0.423
0.438IleCys: 0.438 ± 0.162
3.89IleAsp: 3.89 ± 0.418
2.959IleGlu: 2.959 ± 0.36
1.589IlePhe: 1.589 ± 0.303
4.219IleGly: 4.219 ± 0.546
1.151IleHis: 1.151 ± 0.305
3.616IleIle: 3.616 ± 0.377
3.506IleLys: 3.506 ± 0.481
2.959IleLeu: 2.959 ± 0.317
1.315IleMet: 1.315 ± 0.297
2.52IleAsn: 2.52 ± 0.298
2.246IlePro: 2.246 ± 0.344
1.37IleGln: 1.37 ± 0.22
4.109IleArg: 4.109 ± 0.46
3.068IleSer: 3.068 ± 0.367
3.452IleThr: 3.452 ± 0.476
4.0IleVal: 4.0 ± 0.494
0.384IleTrp: 0.384 ± 0.153
1.698IleTyr: 1.698 ± 0.402
0.0IleXaa: 0.0 ± 0.0
Lys
6.246LysAla: 6.246 ± 0.69
0.493LysCys: 0.493 ± 0.168
2.849LysAsp: 2.849 ± 0.363
3.397LysGlu: 3.397 ± 0.503
1.863LysPhe: 1.863 ± 0.27
3.561LysGly: 3.561 ± 0.421
1.151LysHis: 1.151 ± 0.232
2.301LysIle: 2.301 ± 0.426
3.78LysLys: 3.78 ± 0.491
3.397LysLeu: 3.397 ± 0.442
1.534LysMet: 1.534 ± 0.32
2.739LysAsn: 2.739 ± 0.413
2.575LysPro: 2.575 ± 0.327
1.644LysGln: 1.644 ± 0.314
3.671LysArg: 3.671 ± 0.55
2.739LysSer: 2.739 ± 0.329
3.89LysThr: 3.89 ± 0.495
3.561LysVal: 3.561 ± 0.472
1.26LysTrp: 1.26 ± 0.284
1.479LysTyr: 1.479 ± 0.303
0.0LysXaa: 0.0 ± 0.0
Leu
7.89LeuAla: 7.89 ± 0.858
1.205LeuCys: 1.205 ± 0.251
4.876LeuAsp: 4.876 ± 0.489
3.78LeuGlu: 3.78 ± 0.463
2.959LeuPhe: 2.959 ± 0.402
4.547LeuGly: 4.547 ± 0.756
0.657LeuHis: 0.657 ± 0.198
4.219LeuIle: 4.219 ± 0.456
4.109LeuLys: 4.109 ± 0.546
5.588LeuLeu: 5.588 ± 0.584
2.63LeuMet: 2.63 ± 0.464
3.726LeuAsn: 3.726 ± 0.548
3.726LeuPro: 3.726 ± 0.47
3.561LeuGln: 3.561 ± 0.773
5.588LeuArg: 5.588 ± 0.592
4.602LeuSer: 4.602 ± 0.534
4.986LeuThr: 4.986 ± 0.676
4.602LeuVal: 4.602 ± 0.45
1.425LeuTrp: 1.425 ± 0.247
2.301LeuTyr: 2.301 ± 0.319
0.0LeuXaa: 0.0 ± 0.0
Met
3.397MetAla: 3.397 ± 0.452
0.384MetCys: 0.384 ± 0.174
1.753MetAsp: 1.753 ± 0.304
1.425MetGlu: 1.425 ± 0.277
0.657MetPhe: 0.657 ± 0.174
1.698MetGly: 1.698 ± 0.322
0.438MetHis: 0.438 ± 0.132
1.315MetIle: 1.315 ± 0.241
1.918MetLys: 1.918 ± 0.298
2.301MetLeu: 2.301 ± 0.386
0.712MetMet: 0.712 ± 0.182
1.425MetAsn: 1.425 ± 0.299
1.37MetPro: 1.37 ± 0.273
0.822MetGln: 0.822 ± 0.159
2.246MetArg: 2.246 ± 0.293
2.137MetSer: 2.137 ± 0.347
2.465MetThr: 2.465 ± 0.347
2.301MetVal: 2.301 ± 0.336
0.384MetTrp: 0.384 ± 0.139
0.822MetTyr: 0.822 ± 0.211
0.0MetXaa: 0.0 ± 0.0
Asn
4.767AsnAla: 4.767 ± 0.512
0.329AsnCys: 0.329 ± 0.141
2.027AsnAsp: 2.027 ± 0.358
2.137AsnGlu: 2.137 ± 0.39
1.205AsnPhe: 1.205 ± 0.206
4.438AsnGly: 4.438 ± 0.499
0.548AsnHis: 0.548 ± 0.196
2.411AsnIle: 2.411 ± 0.431
1.644AsnLys: 1.644 ± 0.322
3.178AsnLeu: 3.178 ± 0.355
1.425AsnMet: 1.425 ± 0.238
1.37AsnAsn: 1.37 ± 0.307
2.575AsnPro: 2.575 ± 0.362
0.986AsnGln: 0.986 ± 0.225
2.411AsnArg: 2.411 ± 0.461
2.465AsnSer: 2.465 ± 0.451
2.575AsnThr: 2.575 ± 0.366
2.301AsnVal: 2.301 ± 0.389
1.26AsnTrp: 1.26 ± 0.268
2.027AsnTyr: 2.027 ± 0.396
0.0AsnXaa: 0.0 ± 0.0
Pro
4.602ProAla: 4.602 ± 0.701
0.438ProCys: 0.438 ± 0.174
3.671ProAsp: 3.671 ± 0.512
3.397ProGlu: 3.397 ± 0.503
1.863ProPhe: 1.863 ± 0.29
3.342ProGly: 3.342 ± 0.457
0.931ProHis: 0.931 ± 0.184
1.425ProIle: 1.425 ± 0.296
1.698ProLys: 1.698 ± 0.32
2.849ProLeu: 2.849 ± 0.315
1.041ProMet: 1.041 ± 0.27
1.918ProAsn: 1.918 ± 0.333
1.534ProPro: 1.534 ± 0.372
1.37ProGln: 1.37 ± 0.262
2.246ProArg: 2.246 ± 0.4
2.575ProSer: 2.575 ± 0.368
2.465ProThr: 2.465 ± 0.32
3.616ProVal: 3.616 ± 0.463
0.822ProTrp: 0.822 ± 0.216
1.698ProTyr: 1.698 ± 0.369
0.0ProXaa: 0.0 ± 0.0
Gln
3.726GlnAla: 3.726 ± 0.889
0.274GlnCys: 0.274 ± 0.116
2.137GlnAsp: 2.137 ± 0.322
1.37GlnGlu: 1.37 ± 0.311
1.096GlnPhe: 1.096 ± 0.21
1.972GlnGly: 1.972 ± 0.302
0.603GlnHis: 0.603 ± 0.195
1.863GlnIle: 1.863 ± 0.332
1.479GlnLys: 1.479 ± 0.339
3.452GlnLeu: 3.452 ± 0.572
1.534GlnMet: 1.534 ± 0.286
1.589GlnAsn: 1.589 ± 0.288
1.151GlnPro: 1.151 ± 0.212
1.918GlnGln: 1.918 ± 0.835
2.849GlnArg: 2.849 ± 0.426
1.753GlnSer: 1.753 ± 0.328
1.972GlnThr: 1.972 ± 0.305
2.465GlnVal: 2.465 ± 0.348
0.877GlnTrp: 0.877 ± 0.26
1.151GlnTyr: 1.151 ± 0.287
0.0GlnXaa: 0.0 ± 0.0
Arg
6.41ArgAla: 6.41 ± 0.599
0.603ArgCys: 0.603 ± 0.2
4.767ArgAsp: 4.767 ± 0.461
3.397ArgGlu: 3.397 ± 0.43
2.246ArgPhe: 2.246 ± 0.357
4.164ArgGly: 4.164 ± 0.547
1.151ArgHis: 1.151 ± 0.213
3.068ArgIle: 3.068 ± 0.365
3.561ArgLys: 3.561 ± 0.359
4.767ArgLeu: 4.767 ± 0.561
1.479ArgMet: 1.479 ± 0.293
3.013ArgAsn: 3.013 ± 0.412
3.068ArgPro: 3.068 ± 0.386
2.575ArgGln: 2.575 ± 0.497
4.274ArgArg: 4.274 ± 0.775
2.301ArgSer: 2.301 ± 0.44
2.794ArgThr: 2.794 ± 0.398
5.15ArgVal: 5.15 ± 0.464
0.822ArgTrp: 0.822 ± 0.185
2.192ArgTyr: 2.192 ± 0.36
0.0ArgXaa: 0.0 ± 0.0
Ser
5.369SerAla: 5.369 ± 0.435
0.548SerCys: 0.548 ± 0.182
3.342SerAsp: 3.342 ± 0.424
2.411SerGlu: 2.411 ± 0.376
2.301SerPhe: 2.301 ± 0.503
5.26SerGly: 5.26 ± 0.546
0.712SerHis: 0.712 ± 0.155
2.849SerIle: 2.849 ± 0.323
2.685SerLys: 2.685 ± 0.457
4.821SerLeu: 4.821 ± 0.514
1.425SerMet: 1.425 ± 0.259
2.356SerAsn: 2.356 ± 0.359
2.301SerPro: 2.301 ± 0.403
1.644SerGln: 1.644 ± 0.221
2.63SerArg: 2.63 ± 0.355
3.561SerSer: 3.561 ± 0.63
2.849SerThr: 2.849 ± 0.52
4.493SerVal: 4.493 ± 0.44
0.931SerTrp: 0.931 ± 0.256
1.753SerTyr: 1.753 ± 0.345
0.0SerXaa: 0.0 ± 0.0
Thr
5.479ThrAla: 5.479 ± 0.675
0.329ThrCys: 0.329 ± 0.122
3.78ThrAsp: 3.78 ± 0.48
3.287ThrGlu: 3.287 ± 0.396
2.137ThrPhe: 2.137 ± 0.474
5.205ThrGly: 5.205 ± 0.687
0.986ThrHis: 0.986 ± 0.25
2.794ThrIle: 2.794 ± 0.477
2.904ThrLys: 2.904 ± 0.496
5.698ThrLeu: 5.698 ± 0.6
1.479ThrMet: 1.479 ± 0.293
2.192ThrAsn: 2.192 ± 0.473
3.233ThrPro: 3.233 ± 0.474
2.246ThrGln: 2.246 ± 0.396
2.685ThrArg: 2.685 ± 0.365
3.068ThrSer: 3.068 ± 0.353
3.835ThrThr: 3.835 ± 0.599
5.479ThrVal: 5.479 ± 0.578
1.315ThrTrp: 1.315 ± 0.241
1.589ThrTyr: 1.589 ± 0.296
0.0ThrXaa: 0.0 ± 0.0
Val
6.629ValAla: 6.629 ± 0.636
0.712ValCys: 0.712 ± 0.213
4.109ValAsp: 4.109 ± 0.486
4.438ValGlu: 4.438 ± 0.35
2.575ValPhe: 2.575 ± 0.315
4.876ValGly: 4.876 ± 0.621
1.26ValHis: 1.26 ± 0.336
4.054ValIle: 4.054 ± 0.461
4.328ValLys: 4.328 ± 0.453
5.205ValLeu: 5.205 ± 0.569
3.123ValMet: 3.123 ± 0.36
3.068ValAsn: 3.068 ± 0.473
3.013ValPro: 3.013 ± 0.48
2.192ValGln: 2.192 ± 0.38
4.219ValArg: 4.219 ± 0.489
4.493ValSer: 4.493 ± 0.473
4.602ValThr: 4.602 ± 0.436
5.15ValVal: 5.15 ± 0.568
1.26ValTrp: 1.26 ± 0.292
1.808ValTyr: 1.808 ± 0.293
0.0ValXaa: 0.0 ± 0.0
Trp
2.137TrpAla: 2.137 ± 0.297
0.384TrpCys: 0.384 ± 0.154
1.644TrpAsp: 1.644 ± 0.335
1.096TrpGlu: 1.096 ± 0.276
1.151TrpPhe: 1.151 ± 0.262
1.096TrpGly: 1.096 ± 0.233
0.493TrpHis: 0.493 ± 0.192
0.931TrpIle: 0.931 ± 0.222
1.041TrpLys: 1.041 ± 0.199
1.479TrpLeu: 1.479 ± 0.327
0.274TrpMet: 0.274 ± 0.105
0.493TrpAsn: 0.493 ± 0.149
1.26TrpPro: 1.26 ± 0.301
0.767TrpGln: 0.767 ± 0.2
0.822TrpArg: 0.822 ± 0.224
0.931TrpSer: 0.931 ± 0.206
1.151TrpThr: 1.151 ± 0.306
1.205TrpVal: 1.205 ± 0.253
0.384TrpTrp: 0.384 ± 0.142
0.329TrpTyr: 0.329 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.013TyrAla: 3.013 ± 0.318
0.11TyrCys: 0.11 ± 0.072
2.52TyrAsp: 2.52 ± 0.364
2.192TyrGlu: 2.192 ± 0.391
0.877TyrPhe: 0.877 ± 0.22
2.959TyrGly: 2.959 ± 0.462
0.548TyrHis: 0.548 ± 0.17
1.425TyrIle: 1.425 ± 0.254
1.37TyrLys: 1.37 ± 0.27
2.465TyrLeu: 2.465 ± 0.409
1.096TyrMet: 1.096 ± 0.248
1.698TyrAsn: 1.698 ± 0.34
1.808TyrPro: 1.808 ± 0.351
1.315TyrGln: 1.315 ± 0.31
2.246TyrArg: 2.246 ± 0.346
1.644TyrSer: 1.644 ± 0.286
2.027TyrThr: 2.027 ± 0.378
2.246TyrVal: 2.246 ± 0.353
0.548TyrTrp: 0.548 ± 0.211
1.315TyrTyr: 1.315 ± 0.275
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (18253 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski