Streptococcus phage P9903
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A286QSA5|A0A286QSA5_9CAUD Tail component OS=Streptococcus phage P9903 OX=1971449 GN=P9903_11 PE=4 SV=1
MM1 pKa = 7.3 SVSKK5 pKa = 9.08 EE6 pKa = 4.26 TIMQTLNLDD15 pKa = 3.8 EE16 pKa = 4.97 TDD18 pKa = 3.78 DD19 pKa = 3.8 TALIPAYY26 pKa = 9.65 IEE28 pKa = 3.9 SARR31 pKa = 11.84 QYY33 pKa = 10.3 VVNSVGDD40 pKa = 3.54 DD41 pKa = 3.37 PKK43 pKa = 10.95 FYY45 pKa = 11.07 NLDD48 pKa = 3.41 SVRR51 pKa = 11.84 ALFDD55 pKa = 3.34 TAVIALTSSYY65 pKa = 8.66 FTYY68 pKa = 10.07 RR69 pKa = 11.84 VALTDD74 pKa = 3.37 TATYY78 pKa = 9.41 PVNLTLNSIIGQLRR92 pKa = 11.84 GLYY95 pKa = 8.66 ATYY98 pKa = 10.56 SEE100 pKa = 4.46 EE101 pKa = 5.06 RR102 pKa = 11.84 GDD104 pKa = 3.53
Molecular weight: 11.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.863
IPC2_protein 4.151
IPC_protein 4.062
Toseland 3.859
ProMoST 4.139
Dawson 4.062
Bjellqvist 4.279
Wikipedia 3.999
Rodwell 3.897
Grimsley 3.77
Solomon 4.05
Lehninger 3.999
Nozaki 4.177
DTASelect 4.406
Thurlkill 3.923
EMBOSS 4.012
Sillero 4.19
Patrickios 1.964
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.1
Protein with the highest isoelectric point:
>tr|A0A286QT78|A0A286QT78_9CAUD Head-tail connector protein OS=Streptococcus phage P9903 OX=1971449 GN=P9903_05 PE=4 SV=1
MM1 pKa = 7.62 HH2 pKa = 7.43 KK3 pKa = 9.84 PSKK6 pKa = 10.64 YY7 pKa = 9.16 FIKK10 pKa = 10.51 RR11 pKa = 11.84 RR12 pKa = 11.84 IQYY15 pKa = 10.21 ILFLVQPCIAGSKK28 pKa = 8.79 TGII31 pKa = 3.68
Molecular weight: 3.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.268
IPC2_protein 9.911
IPC_protein 10.16
Toseland 10.526
ProMoST 10.116
Dawson 10.657
Bjellqvist 10.306
Wikipedia 10.804
Rodwell 11.242
Grimsley 10.716
Solomon 10.701
Lehninger 10.672
Nozaki 10.511
DTASelect 10.292
Thurlkill 10.526
EMBOSS 10.906
Sillero 10.57
Patrickios 11.082
IPC_peptide 10.701
IPC2_peptide 9.194
IPC2.peptide.svr19 8.471
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
50
0
50
10962
31
1656
219.2
24.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.523 ± 0.475
0.556 ± 0.106
6.477 ± 0.218
7.015 ± 0.642
4.379 ± 0.193
6.431 ± 0.551
1.423 ± 0.105
6.486 ± 0.203
8.602 ± 0.597
8.128 ± 0.426
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.472 ± 0.15
6.641 ± 0.276
2.864 ± 0.175
4.424 ± 0.251
4.069 ± 0.281
6.094 ± 0.289
5.948 ± 0.26
5.984 ± 0.316
1.35 ± 0.163
4.132 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here