Arthrobacter phage Maureen
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G2KHP4|A0A3G2KHP4_9CAUD Head-to-tail adaptor OS=Arthrobacter phage Maureen OX=2419961 GN=7 PE=4 SV=1
MM1 pKa = 7.25 LHH3 pKa = 7.34 DD4 pKa = 3.97 VDD6 pKa = 4.07 QTPVFATLCLEE17 pKa = 4.2 YY18 pKa = 10.1 PDD20 pKa = 5.67 LVWDD24 pKa = 3.8 QPYY27 pKa = 10.1 VEE29 pKa = 4.26 ARR31 pKa = 11.84 SWDD34 pKa = 3.52 GATVRR39 pKa = 11.84 VVPAGPLTTLL49 pKa = 3.77
Molecular weight: 5.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.809
IPC2_protein 4.088
IPC_protein 3.91
Toseland 3.706
ProMoST 4.113
Dawson 3.923
Bjellqvist 4.088
Wikipedia 3.91
Rodwell 3.745
Grimsley 3.63
Solomon 3.884
Lehninger 3.846
Nozaki 4.075
DTASelect 4.291
Thurlkill 3.808
EMBOSS 3.91
Sillero 4.037
Patrickios 1.952
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.933
Protein with the highest isoelectric point:
>tr|A0A3G2KHU0|A0A3G2KHU0_9CAUD Uncharacterized protein OS=Arthrobacter phage Maureen OX=2419961 GN=57 PE=4 SV=1
MM1 pKa = 7.4 LADD4 pKa = 4.22 LALFAWACVAWAAIILAPCWAVAVLDD30 pKa = 3.46 GWIRR34 pKa = 11.84 KK35 pKa = 8.62 RR36 pKa = 11.84 RR37 pKa = 11.84 AARR40 pKa = 11.84 RR41 pKa = 11.84 DD42 pKa = 3.21 RR43 pKa = 11.84 MLL45 pKa = 5.0
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.313
IPC2_protein 9.253
IPC_protein 10.218
Toseland 10.774
ProMoST 10.76
Dawson 10.789
Bjellqvist 10.613
Wikipedia 11.082
Rodwell 10.73
Grimsley 10.804
Solomon 11.111
Lehninger 11.052
Nozaki 10.818
DTASelect 10.584
Thurlkill 10.76
EMBOSS 11.199
Sillero 10.774
Patrickios 10.716
IPC_peptide 11.111
IPC2_peptide 10.218
IPC2.peptide.svr19 8.89
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
69
0
69
14338
36
1076
207.8
22.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.612 ± 0.561
0.572 ± 0.106
7.421 ± 0.33
5.05 ± 0.328
2.832 ± 0.18
8.683 ± 0.356
1.527 ± 0.151
3.641 ± 0.275
3.341 ± 0.194
8.579 ± 0.281
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.632 ± 0.1
1.848 ± 0.224
5.447 ± 0.277
2.622 ± 0.133
7.923 ± 0.571
5.259 ± 0.27
6.34 ± 0.366
8.321 ± 0.302
1.806 ± 0.128
2.546 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here