Molossus molossus circovirus 4
Average proteome isoelectric point is 7.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I2MP67|A0A2I2MP67_9CIRC Capsid OS=Molossus molossus circovirus 4 OX=1959845 GN=Cap PE=4 SV=1
MM1 pKa = 7.27 SLSRR5 pKa = 11.84 TWCFTSFDD13 pKa = 4.28 DD14 pKa = 3.63 CWEE17 pKa = 4.1 YY18 pKa = 11.39 EE19 pKa = 4.54 PIQQHH24 pKa = 3.98 ITYY27 pKa = 9.37 VVWQIEE33 pKa = 4.29 KK34 pKa = 10.94 CPDD37 pKa = 3.03 TGRR40 pKa = 11.84 LHH42 pKa = 6.0 AQGYY46 pKa = 10.19 LEE48 pKa = 4.17 TKK50 pKa = 10.56 KK51 pKa = 10.44 MISHH55 pKa = 6.93 KK56 pKa = 8.13 EE57 pKa = 3.8 AKK59 pKa = 10.32 SLIFKK64 pKa = 10.38 NNEE67 pKa = 3.49 HH68 pKa = 5.92 FEE70 pKa = 4.13 KK71 pKa = 10.69 RR72 pKa = 11.84 KK73 pKa = 10.18 GSQKK77 pKa = 10.28 QAIDD81 pKa = 3.6 YY82 pKa = 8.78 CKK84 pKa = 10.91 KK85 pKa = 10.34 NDD87 pKa = 3.48 TRR89 pKa = 11.84 EE90 pKa = 4.26 DD91 pKa = 4.37 GPWEE95 pKa = 3.71 WGQPTKK101 pKa = 10.49 QGQRR105 pKa = 11.84 NDD107 pKa = 4.02 LIAIKK112 pKa = 10.92 NMIDD116 pKa = 3.35 EE117 pKa = 4.43 GHH119 pKa = 6.77 TDD121 pKa = 3.61 LEE123 pKa = 4.7 VAQEE127 pKa = 4.71 HH128 pKa = 5.98 FGSWIRR134 pKa = 11.84 YY135 pKa = 6.84 NKK137 pKa = 10.58 SFDD140 pKa = 3.67 KK141 pKa = 10.92 YY142 pKa = 10.97 SKK144 pKa = 9.62 LTLRR148 pKa = 11.84 CRR150 pKa = 11.84 EE151 pKa = 4.03 EE152 pKa = 4.29 PPTVIILWGPAGCGKK167 pKa = 9.26 TSFVYY172 pKa = 10.44 KK173 pKa = 9.38 KK174 pKa = 9.01 TSFVYY179 pKa = 8.56 KK180 pKa = 7.29 THH182 pKa = 5.78 KK183 pKa = 9.13 TVYY186 pKa = 10.69 SKK188 pKa = 11.4 DD189 pKa = 3.32 NSKK192 pKa = 8.85 WWDD195 pKa = 3.77 GYY197 pKa = 10.02 HH198 pKa = 4.72 QQEE201 pKa = 5.2 AILFDD206 pKa = 4.99 DD207 pKa = 5.12 INWNDD212 pKa = 3.43 LDD214 pKa = 3.78 RR215 pKa = 11.84 RR216 pKa = 11.84 EE217 pKa = 4.44 ILLLCDD223 pKa = 4.38 RR224 pKa = 11.84 YY225 pKa = 10.09 PLKK228 pKa = 10.99 KK229 pKa = 9.59 EE230 pKa = 3.93 FKK232 pKa = 10.13 GGYY235 pKa = 8.59 VEE237 pKa = 5.22 INSPYY242 pKa = 10.21 IYY244 pKa = 9.36 FTSNFNPEE252 pKa = 4.07 GYY254 pKa = 10.07 QIYY257 pKa = 10.7 DD258 pKa = 3.44 DD259 pKa = 3.82 ALKK262 pKa = 10.72 RR263 pKa = 11.84 RR264 pKa = 11.84 INEE267 pKa = 4.43 IIPMM271 pKa = 4.39
Molecular weight: 32.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.664
IPC2_protein 6.839
IPC_protein 6.912
Toseland 6.781
ProMoST 7.307
Dawson 7.527
Bjellqvist 7.585
Wikipedia 7.454
Rodwell 7.527
Grimsley 6.868
Solomon 7.585
Lehninger 7.6
Nozaki 7.863
DTASelect 7.702
Thurlkill 7.746
EMBOSS 7.79
Sillero 7.966
Patrickios 4.113
IPC_peptide 7.585
IPC2_peptide 7.102
IPC2.peptide.svr19 7.162
Protein with the highest isoelectric point:
>tr|A0A2I2MP67|A0A2I2MP67_9CIRC Capsid OS=Molossus molossus circovirus 4 OX=1959845 GN=Cap PE=4 SV=1
MM1 pKa = 7.5 PFKK4 pKa = 10.49 RR5 pKa = 11.84 KK6 pKa = 8.71 YY7 pKa = 9.75 RR8 pKa = 11.84 RR9 pKa = 11.84 LRR11 pKa = 11.84 RR12 pKa = 11.84 PAKK15 pKa = 8.8 KK16 pKa = 9.47 RR17 pKa = 11.84 RR18 pKa = 11.84 VITRR22 pKa = 11.84 PRR24 pKa = 11.84 YY25 pKa = 7.16 RR26 pKa = 11.84 TLARR30 pKa = 11.84 GMTANKK36 pKa = 10.27 AGIYY40 pKa = 7.97 WFKK43 pKa = 10.53 RR44 pKa = 11.84 TLNASAEE51 pKa = 4.19 NGTGISVDD59 pKa = 3.33 GTGILQTADD68 pKa = 3.41 MFYY71 pKa = 10.23 MRR73 pKa = 11.84 CNNTAAGSAVFGTMTYY89 pKa = 10.31 QFSLQNIPNISEE101 pKa = 4.1 FTSLFDD107 pKa = 3.29 SYY109 pKa = 10.85 QIRR112 pKa = 11.84 KK113 pKa = 8.92 VVINIIPYY121 pKa = 9.89 NNTVQAAQATNVGVAAAYY139 pKa = 9.0 WPDD142 pKa = 3.98 FSPIMHH148 pKa = 6.12 YY149 pKa = 10.84 VIDD152 pKa = 4.31 HH153 pKa = 7.15 DD154 pKa = 4.24 GVPPVSANEE163 pKa = 3.72 AGINGFQQYY172 pKa = 10.77 ASYY175 pKa = 10.52 KK176 pKa = 8.65 RR177 pKa = 11.84 RR178 pKa = 11.84 RR179 pKa = 11.84 FTGIGPKK186 pKa = 10.2 GLSVVIKK193 pKa = 10.23 PRR195 pKa = 11.84 AKK197 pKa = 10.49 LLAGNAALTTVGGTIAGRR215 pKa = 11.84 NQWQDD220 pKa = 3.19 CAEE223 pKa = 4.06 NNLPHH228 pKa = 6.8 FGFGMILEE236 pKa = 5.29 GISPGTAGAGTIFEE250 pKa = 4.62 VPFRR254 pKa = 11.84 MEE256 pKa = 3.31 TTYY259 pKa = 11.21 YY260 pKa = 11.01 LKK262 pKa = 10.75 FKK264 pKa = 9.87 TIRR267 pKa = 3.41
Molecular weight: 29.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.853
IPC_protein 10.452
Toseland 10.335
ProMoST 10.16
Dawson 10.54
Bjellqvist 10.262
Wikipedia 10.745
Rodwell 10.833
Grimsley 10.628
Solomon 10.584
Lehninger 10.54
Nozaki 10.321
DTASelect 10.248
Thurlkill 10.394
EMBOSS 10.745
Sillero 10.452
Patrickios 10.423
IPC_peptide 10.57
IPC2_peptide 9.151
IPC2.peptide.svr19 8.559
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
538
267
271
269.0
30.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.506 ± 2.506
1.673 ± 0.642
4.833 ± 1.536
5.019 ± 1.665
5.019 ± 0.416
7.435 ± 1.34
2.045 ± 0.64
7.621 ± 0.09
7.435 ± 1.783
5.39 ± 0.362
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.23 ± 0.532
5.576 ± 0.549
4.647 ± 0.415
4.275 ± 0.368
6.32 ± 1.074
5.019 ± 0.364
6.877 ± 0.947
4.275 ± 0.933
2.416 ± 0.898
5.39 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here