Odonata-associated circular virus-11
Average proteome isoelectric point is 8.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UH45|A0A0B4UH45_9VIRU Coat protein OS=Odonata-associated circular virus-11 OX=1592111 PE=4 SV=1
MM1 pKa = 6.92 GHH3 pKa = 7.07 EE4 pKa = 4.15 IRR6 pKa = 11.84 AKK8 pKa = 9.98 SRR10 pKa = 11.84 GWCFTLNNYY19 pKa = 10.41 SNLEE23 pKa = 3.68 ISEE26 pKa = 4.19 LKK28 pKa = 10.71 LRR30 pKa = 11.84 FEE32 pKa = 4.18 GAVWYY37 pKa = 10.02 IIGEE41 pKa = 4.27 EE42 pKa = 4.23 VGLSGTPHH50 pKa = 6.17 LQGAIYY56 pKa = 9.63 YY57 pKa = 9.18 KK58 pKa = 10.57 NAVVMPKK65 pKa = 10.15 FNPRR69 pKa = 11.84 IHH71 pKa = 6.22 WEE73 pKa = 3.85 AMRR76 pKa = 11.84 GNCQQSRR83 pKa = 11.84 EE84 pKa = 4.18 YY85 pKa = 10.55 CSKK88 pKa = 10.62 EE89 pKa = 3.81 KK90 pKa = 11.11 VLVEE94 pKa = 4.17 YY95 pKa = 10.52 GEE97 pKa = 4.46 CPKK100 pKa = 10.51 KK101 pKa = 10.79 GKK103 pKa = 10.44 RR104 pKa = 11.84 SDD106 pKa = 3.24 IKK108 pKa = 11.59 SMVEE112 pKa = 3.59 MLQAGATDD120 pKa = 3.39 KK121 pKa = 10.82 DD122 pKa = 3.28 IYY124 pKa = 9.21 MAHH127 pKa = 6.97 GDD129 pKa = 3.2 KK130 pKa = 10.77 ALRR133 pKa = 11.84 INGCLARR140 pKa = 11.84 ARR142 pKa = 11.84 ASLTEE147 pKa = 4.1 VKK149 pKa = 10.42 RR150 pKa = 11.84 NWEE153 pKa = 3.61 MDD155 pKa = 2.51 IRR157 pKa = 11.84 IYY159 pKa = 10.95 YY160 pKa = 7.58 GAPRR164 pKa = 11.84 TGKK167 pKa = 7.55 TRR169 pKa = 11.84 AVYY172 pKa = 10.55 EE173 pKa = 3.99 EE174 pKa = 4.11 FGIDD178 pKa = 3.24 NVYY181 pKa = 11.28 SKK183 pKa = 10.41 MAGKK187 pKa = 8.48 WWDD190 pKa = 3.69 GYY192 pKa = 10.69 RR193 pKa = 11.84 GEE195 pKa = 4.39 PCVLIDD201 pKa = 6.23 DD202 pKa = 5.74 FDD204 pKa = 4.2 PQNCFDD210 pKa = 3.77 IQYY213 pKa = 11.04 DD214 pKa = 4.67 FYY216 pKa = 11.79 LKK218 pKa = 10.97 LLDD221 pKa = 4.5 RR222 pKa = 11.84 YY223 pKa = 9.37 PLRR226 pKa = 11.84 IEE228 pKa = 4.12 FKK230 pKa = 9.63 GGSGEE235 pKa = 4.44 FYY237 pKa = 10.66 SKK239 pKa = 9.95 TIILTSNFDD248 pKa = 3.52 PAIWFTEE255 pKa = 3.91 KK256 pKa = 10.82 ANRR259 pKa = 11.84 DD260 pKa = 3.29 AFFEE264 pKa = 4.47 RR265 pKa = 11.84 IKK267 pKa = 10.11 EE268 pKa = 3.65 IRR270 pKa = 11.84 YY271 pKa = 9.22 FGGTEE276 pKa = 3.69 VSGGNTSLLTPEE288 pKa = 4.72 NKK290 pKa = 8.32 PTYY293 pKa = 9.88 EE294 pKa = 4.87 GIGKK298 pKa = 9.62
Molecular weight: 34.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.053
IPC2_protein 7.424
IPC_protein 7.337
Toseland 6.942
ProMoST 8.009
Dawson 8.024
Bjellqvist 8.317
Wikipedia 7.951
Rodwell 8.039
Grimsley 6.956
Solomon 8.112
Lehninger 8.126
Nozaki 8.478
DTASelect 8.112
Thurlkill 8.156
EMBOSS 8.229
Sillero 8.478
Patrickios 4.139
IPC_peptide 8.097
IPC2_peptide 7.337
IPC2.peptide.svr19 7.587
Protein with the highest isoelectric point:
>tr|A0A0B4UH45|A0A0B4UH45_9VIRU Coat protein OS=Odonata-associated circular virus-11 OX=1592111 PE=4 SV=1
MM1 pKa = 7.93 PYY3 pKa = 10.35 NRR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 TYY9 pKa = 9.77 RR10 pKa = 11.84 RR11 pKa = 11.84 KK12 pKa = 9.28 RR13 pKa = 11.84 NYY15 pKa = 9.38 RR16 pKa = 11.84 RR17 pKa = 11.84 KK18 pKa = 7.85 TSRR21 pKa = 11.84 ASIYY25 pKa = 10.63 GRR27 pKa = 11.84 AGRR30 pKa = 11.84 QLWKK34 pKa = 10.47 DD35 pKa = 3.33 VKK37 pKa = 9.46 TLKK40 pKa = 10.8 NLINVEE46 pKa = 4.17 WKK48 pKa = 9.55 VQNHH52 pKa = 4.82 VTTSTSVPNTGTVMLLNHH70 pKa = 7.02 LALGEE75 pKa = 4.37 DD76 pKa = 3.92 YY77 pKa = 11.2 NNRR80 pKa = 11.84 SGRR83 pKa = 11.84 QVRR86 pKa = 11.84 WKK88 pKa = 10.22 SLQWRR93 pKa = 11.84 CTTQKK98 pKa = 10.26 HH99 pKa = 5.41 ASATSSRR106 pKa = 11.84 VRR108 pKa = 11.84 MLIVIDD114 pKa = 4.27 RR115 pKa = 11.84 QCKK118 pKa = 8.36 QALPSASEE126 pKa = 3.96 ILDD129 pKa = 3.5 SSVAPLIDD137 pKa = 4.01 GFRR140 pKa = 11.84 QLDD143 pKa = 3.23 ARR145 pKa = 11.84 KK146 pKa = 9.82 RR147 pKa = 11.84 FVILKK152 pKa = 10.43 DD153 pKa = 3.83 VMFLLNNDD161 pKa = 3.48 RR162 pKa = 11.84 PEE164 pKa = 4.11 LTKK167 pKa = 10.39 TGYY170 pKa = 10.82 FKK172 pKa = 10.58 MDD174 pKa = 3.15 MKK176 pKa = 10.35 TVYY179 pKa = 10.14 DD180 pKa = 3.95 ASDD183 pKa = 3.36 NGDD186 pKa = 3.06 ITDD189 pKa = 3.91 IYY191 pKa = 11.15 SNSLYY196 pKa = 10.2 MVMIGDD202 pKa = 4.0 EE203 pKa = 4.31 ATNQPTHH210 pKa = 5.29 EE211 pKa = 4.4 TNVRR215 pKa = 11.84 LRR217 pKa = 11.84 YY218 pKa = 9.35 IDD220 pKa = 3.37 NN221 pKa = 4.02
Molecular weight: 25.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.276
IPC2_protein 9.575
IPC_protein 9.999
Toseland 10.248
ProMoST 9.955
Dawson 10.438
Bjellqvist 10.131
Wikipedia 10.628
Rodwell 10.745
Grimsley 10.511
Solomon 10.482
Lehninger 10.438
Nozaki 10.248
DTASelect 10.131
Thurlkill 10.292
EMBOSS 10.657
Sillero 10.35
Patrickios 10.365
IPC_peptide 10.482
IPC2_peptide 8.916
IPC2.peptide.svr19 8.664
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
519
221
298
259.5
30.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.588 ± 0.348
1.734 ± 0.473
5.78 ± 0.575
5.973 ± 1.859
3.468 ± 0.946
7.129 ± 1.744
1.541 ± 0.153
6.166 ± 0.678
7.129 ± 0.453
7.322 ± 0.728
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.083 ± 0.306
5.78 ± 0.833
3.276 ± 0.32
2.89 ± 0.675
8.478 ± 1.359
5.973 ± 0.723
5.973 ± 1.239
5.202 ± 0.904
2.119 ± 0.177
5.395 ± 0.497
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here