Dyella soli
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7160 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R0YNB1|A0A4R0YNB1_9GAMM Tetratricopeptide repeat protein OS=Dyella soli OX=522319 GN=EZM97_24255 PE=4 SV=1
MM1 pKa = 7.07 SQSSTEE7 pKa = 4.04 TGNLRR12 pKa = 11.84 KK13 pKa = 9.17 WMCVVCGFIYY23 pKa = 10.64 DD24 pKa = 3.83 EE25 pKa = 4.46 ALGLPEE31 pKa = 4.85 EE32 pKa = 5.0 GIEE35 pKa = 4.88 PGTRR39 pKa = 11.84 WADD42 pKa = 3.54 VPDD45 pKa = 3.6 TWTCPDD51 pKa = 3.84 CGATKK56 pKa = 10.7 DD57 pKa = 3.9 DD58 pKa = 4.11 FEE60 pKa = 4.95 MIEE63 pKa = 3.83 VDD65 pKa = 3.41
Molecular weight: 7.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.834
IPC_protein 3.732
Toseland 3.541
ProMoST 3.808
Dawson 3.719
Bjellqvist 3.948
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.465
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.024
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A4R0YRP0|A0A4R0YRP0_9GAMM Extracellular solute-binding protein OS=Dyella soli OX=522319 GN=EZM97_28425 PE=4 SV=1
MM1 pKa = 7.17 TPAAPKK7 pKa = 9.87 KK8 pKa = 10.37 ARR10 pKa = 11.84 AKK12 pKa = 10.38 KK13 pKa = 10.04 KK14 pKa = 10.0 SAPAKK19 pKa = 9.75 KK20 pKa = 9.88 AAPAKK25 pKa = 9.91 KK26 pKa = 9.62 AAKK29 pKa = 9.86 KK30 pKa = 9.96 AVKK33 pKa = 8.72 KK34 pKa = 7.73 TAKK37 pKa = 9.74 KK38 pKa = 9.93 AAAKK42 pKa = 9.85 KK43 pKa = 8.81 VAKK46 pKa = 10.1 KK47 pKa = 10.18 AVKK50 pKa = 9.99 KK51 pKa = 10.34 AVKK54 pKa = 8.99 KK55 pKa = 7.97 TVKK58 pKa = 10.03 KK59 pKa = 10.33 AAAKK63 pKa = 9.83 KK64 pKa = 8.68 VAKK67 pKa = 10.25 KK68 pKa = 10.17 AVKK71 pKa = 9.7 KK72 pKa = 10.37 AAAKK76 pKa = 9.98 KK77 pKa = 8.85 VAKK80 pKa = 9.98 KK81 pKa = 9.49 VAKK84 pKa = 10.22 KK85 pKa = 10.11 AVKK88 pKa = 9.97 KK89 pKa = 10.13 GARR92 pKa = 11.84 KK93 pKa = 9.14 AVKK96 pKa = 9.61 KK97 pKa = 10.55 AVAKK101 pKa = 10.29 RR102 pKa = 11.84 VAKK105 pKa = 10.19 KK106 pKa = 9.71 VARR109 pKa = 11.84 KK110 pKa = 8.99 AVKK113 pKa = 9.79 KK114 pKa = 10.33 AVARR118 pKa = 11.84 KK119 pKa = 8.19 VAKK122 pKa = 9.96 KK123 pKa = 10.45 VSSKK127 pKa = 9.26 KK128 pKa = 7.16 TAKK131 pKa = 9.71 KK132 pKa = 9.48 AAVRR136 pKa = 11.84 KK137 pKa = 8.64 VAKK140 pKa = 10.08 KK141 pKa = 10.11 VAGKK145 pKa = 9.9 KK146 pKa = 8.87 AAKK149 pKa = 9.32 AVKK152 pKa = 10.04 RR153 pKa = 11.84 STASKK158 pKa = 9.94 KK159 pKa = 10.67 AGVKK163 pKa = 9.65 KK164 pKa = 10.4 AVRR167 pKa = 11.84 KK168 pKa = 8.79 VAKK171 pKa = 8.78 KK172 pKa = 6.66 TARR175 pKa = 11.84 RR176 pKa = 11.84 KK177 pKa = 9.68
Molecular weight: 18.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.439
IPC2_protein 11.447
IPC_protein 12.793
Toseland 13.042
ProMoST 13.466
Dawson 13.056
Bjellqvist 12.983
Wikipedia 13.481
Rodwell 13.393
Grimsley 13.086
Solomon 13.495
Lehninger 13.393
Nozaki 13.042
DTASelect 12.983
Thurlkill 13.042
EMBOSS 13.525
Sillero 13.042
Patrickios 13.1
IPC_peptide 13.495
IPC2_peptide 12.442
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7160
0
7160
2409684
30
5691
336.5
36.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.297 ± 0.038
0.83 ± 0.009
5.774 ± 0.021
5.061 ± 0.03
3.448 ± 0.016
8.464 ± 0.032
2.479 ± 0.015
4.328 ± 0.023
2.997 ± 0.023
10.638 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.405 ± 0.015
2.691 ± 0.024
5.201 ± 0.024
3.854 ± 0.02
7.158 ± 0.033
5.608 ± 0.027
5.144 ± 0.03
7.577 ± 0.027
1.575 ± 0.013
2.472 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here