Shimia marina

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Shimia

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3874 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N7LRS1|A0A0N7LRS1_9RHOB Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE OS=Shimia marina OX=321267 GN=ubiE PE=3 SV=1
MM1 pKa = 7.98CIMCAATATFDD12 pKa = 4.15PNRR15 pKa = 11.84HH16 pKa = 5.92PGEE19 pKa = 4.59GPVQALITEE28 pKa = 4.7GNDD31 pKa = 2.79AAAGVGTNYY40 pKa = 9.78TISVGDD46 pKa = 3.89TFNGTLDD53 pKa = 3.49RR54 pKa = 11.84AGDD57 pKa = 3.91RR58 pKa = 11.84DD59 pKa = 3.52WVAIEE64 pKa = 4.02LTADD68 pKa = 2.76TWYY71 pKa = 10.53EE72 pKa = 3.8IGLSGAATSSGTLSDD87 pKa = 4.07PYY89 pKa = 11.1LRR91 pKa = 11.84LYY93 pKa = 10.72DD94 pKa = 3.72SSGNLIGEE102 pKa = 4.38NDD104 pKa = 4.29DD105 pKa = 4.13GGQGYY110 pKa = 10.05EE111 pKa = 4.09SLLGFSASYY120 pKa = 9.78TGTYY124 pKa = 9.74YY125 pKa = 10.15IAAGSYY131 pKa = 10.24RR132 pKa = 11.84DD133 pKa = 4.05GGQGTYY139 pKa = 9.89EE140 pKa = 4.04ISVEE144 pKa = 4.14TAVPGTVDD152 pKa = 3.37EE153 pKa = 4.9LADD156 pKa = 3.88FLTEE160 pKa = 4.23GFWGSEE166 pKa = 4.05RR167 pKa = 11.84HH168 pKa = 5.52WNTSSDD174 pKa = 3.67NIISVNITGLTAAGQQLARR193 pKa = 11.84WAFEE197 pKa = 3.49AWEE200 pKa = 4.22MVANLDD206 pKa = 3.74FQEE209 pKa = 4.37TSGAADD215 pKa = 3.11ITFDD219 pKa = 5.24DD220 pKa = 4.72EE221 pKa = 5.77DD222 pKa = 4.34SGAYY226 pKa = 9.17AQSTVGGGFITSSSVNVGTGWLSSYY251 pKa = 9.12GTTLGSYY258 pKa = 9.87SFQTYY263 pKa = 6.68VHH265 pKa = 7.14EE266 pKa = 4.95IGHH269 pKa = 6.89AIGLGHH275 pKa = 6.24QGGYY279 pKa = 9.86NGSATYY285 pKa = 10.36GVDD288 pKa = 3.12EE289 pKa = 4.63TFSNDD294 pKa = 2.68SWQMSIMSYY303 pKa = 10.51FSQSEE308 pKa = 4.22NTTVDD313 pKa = 2.8ASYY316 pKa = 11.63ARR318 pKa = 11.84LLTAMMADD326 pKa = 3.3IVAAQNLYY334 pKa = 10.38GASTSTDD341 pKa = 2.96GNTVWGSGANLGNYY355 pKa = 9.86LDD357 pKa = 4.8DD358 pKa = 5.35AFAALDD364 pKa = 3.92GSGDD368 pKa = 3.44TSIYY372 pKa = 10.61NGGPVALTIVDD383 pKa = 3.58SGGNDD388 pKa = 3.74LLNLRR393 pKa = 11.84PYY395 pKa = 9.44NTDD398 pKa = 2.44SRR400 pKa = 11.84IDD402 pKa = 3.63LTPGTFSDD410 pKa = 4.18INGLTGNLGIARR422 pKa = 11.84GTVIEE427 pKa = 4.92RR428 pKa = 11.84LTTGNGNDD436 pKa = 3.97TITGNWAGNVVNAGGGNDD454 pKa = 4.21SIDD457 pKa = 4.53GGGGWDD463 pKa = 4.21KK464 pKa = 11.34VWAGDD469 pKa = 4.46GNDD472 pKa = 3.45TVLGGEE478 pKa = 4.54GNDD481 pKa = 3.84TLGGMNGDD489 pKa = 3.57DD490 pKa = 4.47RR491 pKa = 11.84LWGGNGGDD499 pKa = 3.71RR500 pKa = 11.84LYY502 pKa = 11.41GGADD506 pKa = 3.41NDD508 pKa = 4.48TLGGGAGEE516 pKa = 4.5DD517 pKa = 3.35RR518 pKa = 11.84LFGGTGHH525 pKa = 6.97DD526 pKa = 3.56TLRR529 pKa = 11.84GNGDD533 pKa = 3.31SDD535 pKa = 3.81FLRR538 pKa = 11.84GGTGNDD544 pKa = 3.43ALFGDD549 pKa = 5.07DD550 pKa = 5.95GNDD553 pKa = 3.32TLLGDD558 pKa = 4.87DD559 pKa = 4.94GQDD562 pKa = 3.21TLIGGNGADD571 pKa = 3.71SLQAGGWADD580 pKa = 3.64VLEE583 pKa = 4.99GGAGADD589 pKa = 3.89TLLGDD594 pKa = 4.64AGSDD598 pKa = 3.57TLRR601 pKa = 11.84GDD603 pKa = 3.74AGNDD607 pKa = 3.44SLSGGSYY614 pKa = 10.74HH615 pKa = 7.96DD616 pKa = 4.31MLNGGADD623 pKa = 3.49NDD625 pKa = 4.03VLHH628 pKa = 7.29GDD630 pKa = 4.24GGNDD634 pKa = 3.44TLFGGAGNDD643 pKa = 3.49TLYY646 pKa = 11.12GGSGNDD652 pKa = 2.89RR653 pKa = 11.84LFFGAGNDD661 pKa = 3.64VGDD664 pKa = 4.57GGLGADD670 pKa = 3.52TFVFGANVGSDD681 pKa = 3.1NTINNFTVSEE691 pKa = 4.11GDD693 pKa = 3.48RR694 pKa = 11.84LSLDD698 pKa = 3.45DD699 pKa = 5.35ALWLSGHH706 pKa = 5.76GTLSAAEE713 pKa = 4.08VLSTFGSTVGGDD725 pKa = 3.41LVLTFDD731 pKa = 4.67GGQSITLSGLGTTAGLEE748 pKa = 4.16SALDD752 pKa = 3.78LFF754 pKa = 5.27

Molecular weight: 76.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P1EKZ3|A0A0P1EKZ3_9RHOB Putative transposase OrfB OS=Shimia marina OX=321267 GN=SHM7688_00244 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3874

0

3874

1193212

30

2003

308.0

33.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.942 ± 0.053

0.907 ± 0.015

5.948 ± 0.034

6.127 ± 0.038

3.86 ± 0.027

8.342 ± 0.037

2.172 ± 0.021

5.139 ± 0.024

3.612 ± 0.035

9.986 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.886 ± 0.018

2.816 ± 0.022

4.746 ± 0.029

3.557 ± 0.024

6.12 ± 0.039

5.391 ± 0.029

5.54 ± 0.032

7.297 ± 0.034

1.356 ± 0.016

2.257 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski