Aliidiomarina soli
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2855 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A432WH25|A0A432WH25_9GAMM Long-chain-fatty-acid--CoA ligase FadD OS=Aliidiomarina soli OX=1928574 GN=CWE14_07235 PE=4 SV=1
MM1 pKa = 7.76 LKK3 pKa = 10.05 PQLSLILKK11 pKa = 9.53 VSSLTLAVLLTGCNSSDD28 pKa = 3.61 EE29 pKa = 4.58 KK30 pKa = 11.28 GDD32 pKa = 3.43 TYY34 pKa = 10.85 TRR36 pKa = 11.84 EE37 pKa = 3.96 VVVIDD42 pKa = 3.73 GQPAIFNGAFFTSLGYY58 pKa = 10.4 SIEE61 pKa = 4.25 RR62 pKa = 11.84 EE63 pKa = 4.12 SGQQTTAPWIEE74 pKa = 4.4 DD75 pKa = 3.16 FTFRR79 pKa = 11.84 WGYY82 pKa = 9.84 QVTARR87 pKa = 11.84 IEE89 pKa = 4.21 VVTYY93 pKa = 10.69 AEE95 pKa = 4.47 PPEE98 pKa = 4.79 DD99 pKa = 4.02 GPMHH103 pKa = 7.36 EE104 pKa = 4.32 YY105 pKa = 10.71 RR106 pKa = 11.84 LLEE109 pKa = 4.21 ILDD112 pKa = 3.81 SSEE115 pKa = 5.22 DD116 pKa = 3.79 EE117 pKa = 4.33 VGTVYY122 pKa = 11.05 SFDD125 pKa = 3.71 GVFLRR130 pKa = 11.84 NDD132 pKa = 2.88 NTFQIDD138 pKa = 3.68 EE139 pKa = 4.67 DD140 pKa = 4.14 NEE142 pKa = 4.53 GYY144 pKa = 11.12 YY145 pKa = 10.92 LFLDD149 pKa = 3.81 EE150 pKa = 6.46 SFVCAQNINCDD161 pKa = 3.56 QLVDD165 pKa = 3.68 MSNDD169 pKa = 2.98 GGTISLEE176 pKa = 3.93 FEE178 pKa = 4.36 YY179 pKa = 10.66 LGQSDD184 pKa = 4.61 DD185 pKa = 3.9 RR186 pKa = 11.84 PTAVMLTDD194 pKa = 3.7 WNN196 pKa = 3.87
Molecular weight: 22.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.783
IPC_protein 3.77
Toseland 3.567
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.592
Grimsley 3.478
Solomon 3.745
Lehninger 3.694
Nozaki 3.872
DTASelect 4.062
Thurlkill 3.605
EMBOSS 3.681
Sillero 3.884
Patrickios 1.062
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.814
Protein with the highest isoelectric point:
>tr|A0A432WIW2|A0A432WIW2_9GAMM Uncharacterized protein OS=Aliidiomarina soli OX=1928574 GN=CWE14_04690 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 7.97 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.94 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2855
0
2855
940704
32
2818
329.5
36.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.811 ± 0.042
0.86 ± 0.017
5.867 ± 0.031
6.38 ± 0.042
3.885 ± 0.033
7.089 ± 0.042
2.324 ± 0.024
5.306 ± 0.035
3.514 ± 0.044
10.736 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.506 ± 0.021
3.569 ± 0.028
4.171 ± 0.026
5.232 ± 0.046
5.974 ± 0.033
6.314 ± 0.039
5.078 ± 0.028
7.101 ± 0.036
1.358 ± 0.02
2.925 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here