Coptotermes formosanus (Formosan subterranean termite)
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12806 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6L2Q6Z9|A0A6L2Q6Z9_COPFO Uncharacterized protein OS=Coptotermes formosanus OX=36987 GN=Cfor_07107 PE=4 SV=1
MM1 pKa = 7.87 KK2 pKa = 9.99 IPNGTGSGYY11 pKa = 10.29 YY12 pKa = 10.27 GHH14 pKa = 7.82 DD15 pKa = 3.47 SSVISYY21 pKa = 10.56 LSDD24 pKa = 3.32 SSDD27 pKa = 3.24 VGNGDD32 pKa = 4.47 DD33 pKa = 5.11 ADD35 pKa = 4.13 SPTLDD40 pKa = 3.84 GDD42 pKa = 3.65 FSEE45 pKa = 4.66 YY46 pKa = 8.46 MWMEE50 pKa = 3.9 NEE52 pKa = 4.23 EE53 pKa = 4.35 EE54 pKa = 3.91 FDD56 pKa = 3.79 KK57 pKa = 11.43 QVMQQLEE64 pKa = 4.49 EE65 pKa = 4.08 EE66 pKa = 4.6 ALMEE70 pKa = 4.0 QCIEE74 pKa = 4.33 AMLEE78 pKa = 4.05 DD79 pKa = 3.99 EE80 pKa = 5.28 RR81 pKa = 11.84 EE82 pKa = 3.62 IHH84 pKa = 6.59 NIHH87 pKa = 5.91 NQAMAANGVGVIDD100 pKa = 4.99 VRR102 pKa = 11.84 QPWSTGTDD110 pKa = 3.67 YY111 pKa = 11.55 VHH113 pKa = 6.84 PTMM116 pKa = 5.78
Molecular weight: 12.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.846
IPC_protein 3.795
Toseland 3.605
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.516
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.075
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.923
Patrickios 0.947
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.814
Protein with the highest isoelectric point:
>tr|A0A6L2PEC4|A0A6L2PEC4_COPFO Uncharacterized protein OS=Coptotermes formosanus OX=36987 GN=Cfor_03229 PE=4 SV=1
MM1 pKa = 7.47 IRR3 pKa = 11.84 NLRR6 pKa = 11.84 EE7 pKa = 3.71 NNAVQQQASVKK18 pKa = 9.6 NRR20 pKa = 11.84 RR21 pKa = 11.84 LAQQMEE27 pKa = 4.18 RR28 pKa = 11.84 RR29 pKa = 11.84 PAVMAALKK37 pKa = 10.24 LKK39 pKa = 10.33 KK40 pKa = 9.94 RR41 pKa = 11.84 SLRR44 pKa = 11.84 QRR46 pKa = 11.84 LGQPVTSSVKK56 pKa = 10.43 DD57 pKa = 3.58 RR58 pKa = 11.84 LTLAGPTRR66 pKa = 11.84 GGRR69 pKa = 11.84 GNSRR73 pKa = 11.84 AQGRR77 pKa = 11.84 GRR79 pKa = 11.84 SGGRR83 pKa = 11.84 GWGSRR88 pKa = 11.84 LSRR91 pKa = 11.84 SQSMQSVNRR100 pKa = 11.84 SGGGGDD106 pKa = 3.39 TFRR109 pKa = 11.84 LRR111 pKa = 11.84 RR112 pKa = 11.84 GQGRR116 pKa = 11.84 VFRR119 pKa = 11.84 GNNSQRR125 pKa = 11.84 RR126 pKa = 11.84 PFRR129 pKa = 11.84 RR130 pKa = 11.84 GGRR133 pKa = 11.84 PGLGSPRR140 pKa = 11.84 GGRR143 pKa = 11.84 GLRR146 pKa = 11.84 GGRR149 pKa = 11.84 GGSVGNRR156 pKa = 11.84 FARR159 pKa = 11.84 GRR161 pKa = 11.84 GGSFF165 pKa = 3.04
Molecular weight: 17.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.469
IPC2_protein 11.023
IPC_protein 12.632
Toseland 12.793
ProMoST 13.29
Dawson 12.793
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.354
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.793
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.076
IPC_peptide 13.29
IPC2_peptide 12.281
IPC2.peptide.svr19 9.144
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12806
0
12806
6406585
49
14142
500.3
55.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.431 ± 0.021
2.281 ± 0.02
5.25 ± 0.016
6.547 ± 0.031
3.625 ± 0.017
6.023 ± 0.023
2.885 ± 0.012
4.774 ± 0.016
5.747 ± 0.024
9.306 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.285 ± 0.009
4.311 ± 0.017
5.161 ± 0.029
4.459 ± 0.018
5.51 ± 0.019
8.43 ± 0.033
5.89 ± 0.018
6.985 ± 0.017
1.146 ± 0.008
2.954 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here