Salmonella phage vB_SemP_Emek

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus; unclassified Lederbergvirus

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6S1N0|I6S1N0_9CAUD Cro OS=Salmonella phage vB_SemP_Emek OX=1168548 GN=cro PE=4 SV=1
MM1 pKa = 7.26LHH3 pKa = 7.23DD4 pKa = 4.7FDD6 pKa = 6.33DD7 pKa = 4.46EE8 pKa = 4.25EE9 pKa = 5.37FIALSSPEE17 pKa = 3.65IEE19 pKa = 4.47EE20 pKa = 4.42EE21 pKa = 4.19VEE23 pKa = 4.03QQINLAAEE31 pKa = 4.07RR32 pKa = 11.84QNPVISWDD40 pKa = 3.48EE41 pKa = 3.67FAGYY45 pKa = 10.62YY46 pKa = 9.62SS47 pKa = 3.45

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6RSJ2|I6RSJ2_9CAUD NinZ OS=Salmonella phage vB_SemP_Emek OX=1168548 GN=ninZ PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84QRR5 pKa = 11.84RR6 pKa = 11.84SITDD10 pKa = 4.1IICEE14 pKa = 3.96NCKK17 pKa = 10.21YY18 pKa = 10.77LPTKK22 pKa = 10.48RR23 pKa = 11.84SRR25 pKa = 11.84NKK27 pKa = 9.52PKK29 pKa = 10.35PIPTEE34 pKa = 4.16SQVKK38 pKa = 8.12TFDD41 pKa = 3.4YY42 pKa = 11.21VYY44 pKa = 11.4GLLQSKK50 pKa = 7.75WNRR53 pKa = 11.84MRR55 pKa = 11.84KK56 pKa = 7.02TRR58 pKa = 3.69

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

12054

31

725

172.2

19.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.433 ± 0.544

1.211 ± 0.146

5.932 ± 0.226

6.778 ± 0.313

3.219 ± 0.21

7.168 ± 0.399

1.742 ± 0.19

5.923 ± 0.203

6.322 ± 0.292

7.765 ± 0.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.92 ± 0.179

4.762 ± 0.259

3.941 ± 0.199

4.521 ± 0.284

6.139 ± 0.322

6.172 ± 0.271

5.152 ± 0.278

5.782 ± 0.318

1.717 ± 0.16

3.401 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski