Proteome-pI 2.0 - Proteome Isoelectric Point Database

Streptomyces viridosporus (strain ATCC 14672 / DSM 40746 / JCM 4963 / KCTC 9882 / NRRL B-12104 / FH 1290) (Streptomyces ghanaensis)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces viridosporus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 7865 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|D5ZRJ4|D5ZRJ4_STRV1 Sec-independent protein translocase protein TatA OS=Streptomyces viridosporus (strain ATCC 14672 / DSM 40746 / JCM 4963 / KCTC 9882 / NRRL B-12104 / FH 1290) OX=566461 GN=tatA PE=3 SV=1

MM1 pKa = 7.57SVQQEE6 pKa = 4.1AGVDD10 pKa = 4.05GEE12 pKa = 4.74ALEE15 pKa = 4.23VWIDD19 pKa = 3.41QDD21 pKa = 3.99LCTGDD26 pKa = 5.01GICAQYY32 pKa = 10.9APEE35 pKa = 4.23VFEE38 pKa = 5.9LDD40 pKa = 3.05IDD42 pKa = 3.65GLAYY46 pKa = 10.69VKK48 pKa = 10.72GPQDD52 pKa = 3.58EE53 pKa = 4.47LLQDD57 pKa = 3.83RR58 pKa = 11.84GAATPVPLPLLTDD71 pKa = 4.3VIDD74 pKa = 4.0SAKK77 pKa = 9.8EE78 pKa = 3.88CPGEE82 pKa = 4.38CIHH85 pKa = 6.3VRR87 pKa = 11.84RR88 pKa = 11.84VSDD91 pKa = 3.5GVEE94 pKa = 3.69VHH96 pKa = 6.83GPDD99 pKa = 4.44AEE101 pKa = 4.16

Molecular weight: 10.79 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|D5ZR02|D5ZR02_STRV1 Ala_racemase_N domain-containing protein OS=Streptomyces viridosporus (strain ATCC 14672 / DSM 40746 / JCM 4963 / KCTC 9882 / NRRL B-12104 / FH 1290) OX=566461 GN=SSFG_03637 PE=4 SV=1

MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
7865	0	7865	2335780	30	4795	297.0	31.91

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
13.255 ± 0.044	0.872 ± 0.009	5.967 ± 0.023	5.859 ± 0.027	2.69 ± 0.017	9.561 ± 0.029	2.458 ± 0.015	2.943 ± 0.019	2.005 ± 0.024	10.178 ± 0.035

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
1.739 ± 0.012	1.678 ± 0.014	6.41 ± 0.03	2.608 ± 0.017	8.881 ± 0.034	4.888 ± 0.021	6.022 ± 0.021	8.498 ± 0.025	1.496 ± 0.013	1.992 ± 0.013

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski