Mushroom bacilliform virus (isolate Australia/AUS LF-1) (MBV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Barnaviridae; Barnavirus; Mushroom bacilliform virus

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9YPD6|PTASE_MBVLF Putative serine protease OS=Mushroom bacilliform virus (isolate Australia/AUS LF-1) OX=650482 GN=ORF2 PE=3 SV=1
MM1 pKa = 8.48DD2 pKa = 4.88SGLYY6 pKa = 9.61PKK8 pKa = 9.96TGAPTWFYY16 pKa = 10.36GWSKK20 pKa = 9.92QRR22 pKa = 11.84EE23 pKa = 4.4GGFSDD28 pKa = 4.43FGSEE32 pKa = 3.88IFRR35 pKa = 11.84LRR37 pKa = 11.84ALPPLKK43 pKa = 10.79AEE45 pKa = 3.94IFRR48 pKa = 11.84GIRR51 pKa = 11.84EE52 pKa = 4.03VVEE55 pKa = 3.84YY56 pKa = 10.82NITGDD61 pKa = 3.62SHH63 pKa = 7.4PGYY66 pKa = 8.99PWCKK70 pKa = 9.89LGSDD74 pKa = 3.67NKK76 pKa = 10.93AVLTGFGDD84 pKa = 4.55LIWDD88 pKa = 4.0EE89 pKa = 4.01VAKK92 pKa = 10.62RR93 pKa = 11.84FNNMLGYY100 pKa = 10.8GDD102 pKa = 5.99AIFSMTPSEE111 pKa = 4.05LVQNGICDD119 pKa = 3.58AVKK122 pKa = 10.72VFIKK126 pKa = 10.35QEE128 pKa = 3.77PHH130 pKa = 6.4SLEE133 pKa = 3.93KK134 pKa = 11.1VNAGRR139 pKa = 11.84LRR141 pKa = 11.84IIAAVGLVDD150 pKa = 4.84QIVTRR155 pKa = 11.84LLCMKK160 pKa = 10.14QNNAEE165 pKa = 3.86IDD167 pKa = 3.68CWEE170 pKa = 4.5SCPSAPGMGLNDD182 pKa = 3.31EE183 pKa = 4.73GLRR186 pKa = 11.84TLYY189 pKa = 9.67STAQVMAEE197 pKa = 4.36HH198 pKa = 6.23GTICEE203 pKa = 4.16TDD205 pKa = 2.74ISGWDD210 pKa = 3.21WSVQQWEE217 pKa = 4.41LDD219 pKa = 3.01SDD221 pKa = 3.66ARR223 pKa = 11.84LRR225 pKa = 11.84TQLAGEE231 pKa = 4.39EE232 pKa = 3.81IGGYY236 pKa = 10.65LNFFLRR242 pKa = 11.84VHH244 pKa = 6.88AYY246 pKa = 9.62VVGHH250 pKa = 5.83SVFVMPDD257 pKa = 3.08GEE259 pKa = 4.37MLEE262 pKa = 4.14QTVPGGQLSGDD273 pKa = 4.01YY274 pKa = 8.36NTSSSNSRR282 pKa = 11.84MRR284 pKa = 11.84VIATMFARR292 pKa = 11.84YY293 pKa = 8.52LAGQVSGFPLLGIKK307 pKa = 10.55AMGDD311 pKa = 3.26DD312 pKa = 4.81SFEE315 pKa = 3.69IWFKK319 pKa = 11.17GLEE322 pKa = 4.08EE323 pKa = 4.1YY324 pKa = 10.04LGKK327 pKa = 9.87MGHH330 pKa = 4.88TVKK333 pKa = 9.93MCVQRR338 pKa = 11.84PGLVGFEE345 pKa = 4.36FCSQVFLGLGIAYY358 pKa = 8.89PVDD361 pKa = 4.28FSKK364 pKa = 9.95TLYY367 pKa = 10.33RR368 pKa = 11.84FLSHH372 pKa = 6.97HH373 pKa = 6.53PADD376 pKa = 4.18PKK378 pKa = 9.52YY379 pKa = 10.63SEE381 pKa = 4.15YY382 pKa = 10.34RR383 pKa = 11.84AQLMYY388 pKa = 10.46YY389 pKa = 10.03FRR391 pKa = 11.84HH392 pKa = 6.12LPSSTLQKK400 pKa = 10.56VIRR403 pKa = 11.84LAGARR408 pKa = 11.84VEE410 pKa = 4.29RR411 pKa = 11.84AQKK414 pKa = 10.61LATSSNN420 pKa = 3.6

Molecular weight: 46.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9YPD5|RDRP_MBVLF RNA-directed RNA polymerase OS=Mushroom bacilliform virus (isolate Australia/AUS LF-1) OX=650482 GN=ORF3 PE=3 SV=1
MM1 pKa = 7.69ANRR4 pKa = 11.84RR5 pKa = 11.84QSRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84GNKK13 pKa = 8.3NRR15 pKa = 11.84NSATVRR21 pKa = 11.84RR22 pKa = 11.84APPNRR27 pKa = 11.84ATQSSSGKK35 pKa = 9.61VKK37 pKa = 9.93FVKK40 pKa = 10.07WIAASPTKK48 pKa = 10.4LIPHH52 pKa = 6.7IGEE55 pKa = 4.31NEE57 pKa = 3.74TSYY60 pKa = 11.67GVLFDD65 pKa = 3.37ITGTTFPEE73 pKa = 4.63LSSLMSRR80 pKa = 11.84HH81 pKa = 4.32SRR83 pKa = 11.84YY84 pKa = 9.53RR85 pKa = 11.84VLSLGARR92 pKa = 11.84IVPYY96 pKa = 10.58DD97 pKa = 3.97PNCLGAHH104 pKa = 4.84SVKK107 pKa = 10.27VFAEE111 pKa = 4.09SVYY114 pKa = 10.99DD115 pKa = 3.82SSATPTVPSVHH126 pKa = 5.91YY127 pKa = 9.63LQSNGCRR134 pKa = 11.84VVPANKK140 pKa = 9.51QLSSPPSSDD149 pKa = 3.0VNKK152 pKa = 10.51YY153 pKa = 10.35YY154 pKa = 10.26EE155 pKa = 4.24ICSDD159 pKa = 3.65DD160 pKa = 3.82AVIGRR165 pKa = 11.84IMYY168 pKa = 9.46AWNGPGLSTARR179 pKa = 11.84VGFCSFEE186 pKa = 4.14VYY188 pKa = 11.01ADD190 pKa = 4.0LEE192 pKa = 4.15FDD194 pKa = 5.15GIRR197 pKa = 11.84EE198 pKa = 4.09

Molecular weight: 21.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1454

179

657

363.5

40.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.121 ± 0.586

2.338 ± 0.437

4.47 ± 0.217

5.983 ± 0.833

4.402 ± 0.334

7.497 ± 1.024

1.788 ± 0.464

4.195 ± 0.257

5.089 ± 0.579

9.216 ± 0.925

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.82 ± 0.381

3.508 ± 0.697

4.952 ± 0.519

3.164 ± 0.587

6.19 ± 0.699

9.422 ± 1.295

5.433 ± 0.728

8.391 ± 0.679

2.201 ± 0.393

2.82 ± 0.739
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski