Nonomuraea polychroma

Taxonomy: c

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10251 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A438MNE3|A0A438MNE3_9ACTN Type VII secretion integral membrane protein EccD OS=Nonomuraea polychroma OX=46176 GN=EDD27_10272 PE=3 SV=1
MM1 pKa = 7.57SYY3 pKa = 11.33DD4 pKa = 3.35LAVWEE9 pKa = 4.72GEE11 pKa = 4.25RR12 pKa = 11.84PSSDD16 pKa = 3.02QEE18 pKa = 3.97AGEE21 pKa = 4.3THH23 pKa = 5.87SALYY27 pKa = 8.91EE28 pKa = 3.94QYY30 pKa = 10.47IDD32 pKa = 3.72TDD34 pKa = 3.92VEE36 pKa = 4.26IPPTPLIAKK45 pKa = 9.33FVEE48 pKa = 4.16QLLQRR53 pKa = 11.84WPDD56 pKa = 3.52LDD58 pKa = 5.98DD59 pKa = 4.06VDD61 pKa = 5.31EE62 pKa = 6.29DD63 pKa = 4.11DD64 pKa = 6.55DD65 pKa = 4.66EE66 pKa = 6.47YY67 pKa = 11.65EE68 pKa = 4.96DD69 pKa = 4.86IPWSTSPLIGEE80 pKa = 4.06AAGPYY85 pKa = 9.47IYY87 pKa = 10.59FPMGYY92 pKa = 9.14RR93 pKa = 11.84RR94 pKa = 11.84AEE96 pKa = 3.91EE97 pKa = 3.75ASAYY101 pKa = 9.4AVQVAAQLGLNCYY114 pKa = 10.03DD115 pKa = 3.67PQEE118 pKa = 4.34DD119 pKa = 3.9RR120 pKa = 11.84LRR122 pKa = 11.84TT123 pKa = 3.62

Molecular weight: 14.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A438M9I4|A0A438M9I4_9ACTN Carbohydrate ABC transporter substrate-binding protein (CUT1 family) OS=Nonomuraea polychroma OX=46176 GN=EDD27_4970 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 10.4RR3 pKa = 11.84FFKK6 pKa = 10.97AMLLRR11 pKa = 11.84RR12 pKa = 11.84LARR15 pKa = 11.84TPIGAVFLAVGWVLGRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AQRR39 pKa = 11.84RR40 pKa = 11.84GRR42 pKa = 11.84RR43 pKa = 3.47

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10251

0

10251

3320502

29

4646

323.9

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.145 ± 0.04

0.775 ± 0.007

5.742 ± 0.015

5.581 ± 0.024

2.881 ± 0.014

9.17 ± 0.023

2.235 ± 0.014

3.762 ± 0.017

2.218 ± 0.021

10.523 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.962 ± 0.01

1.892 ± 0.016

5.986 ± 0.02

2.836 ± 0.015

7.926 ± 0.03

5.026 ± 0.019

5.906 ± 0.022

8.575 ± 0.021

1.645 ± 0.01

2.214 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski