Streptococcus phage Javan119
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZY94|A0A4D5ZY94_9CAUD Abortive infection bacteriophage resistance protein OS=Streptococcus phage Javan119 OX=2547988 GN=Javan119_0005 PE=4 SV=1
MM1 pKa = 7.16 LTYY4 pKa = 10.89 DD5 pKa = 4.11 EE6 pKa = 5.2 FKK8 pKa = 10.87 QAIDD12 pKa = 3.77 DD13 pKa = 4.54 GYY15 pKa = 9.71 ITGDD19 pKa = 3.05 TVAIVRR25 pKa = 11.84 KK26 pKa = 9.56 NGQIFDD32 pKa = 3.87 YY33 pKa = 10.86 VLPNEE38 pKa = 4.19 EE39 pKa = 4.01 ARR41 pKa = 11.84 NGEE44 pKa = 4.19 VVTDD48 pKa = 3.81 EE49 pKa = 4.15 KK50 pKa = 11.56 VEE52 pKa = 3.97 AVLMEE57 pKa = 4.36 LEE59 pKa = 4.12
Molecular weight: 6.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.266
IPC2_protein 4.037
IPC_protein 3.91
Toseland 3.732
ProMoST 4.012
Dawson 3.884
Bjellqvist 4.126
Wikipedia 3.795
Rodwell 3.757
Grimsley 3.656
Solomon 3.872
Lehninger 3.821
Nozaki 4.012
DTASelect 4.151
Thurlkill 3.783
EMBOSS 3.808
Sillero 4.024
Patrickios 3.592
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.964
Protein with the highest isoelectric point:
>tr|A0A4D5ZZE0|A0A4D5ZZE0_9CAUD Major capsid protein OS=Streptococcus phage Javan119 OX=2547988 GN=Javan119_0028 PE=4 SV=1
MM1 pKa = 7.11 WRR3 pKa = 11.84 KK4 pKa = 9.34 ALSFLYY10 pKa = 9.93 FAEE13 pKa = 4.4 VGPGTRR19 pKa = 11.84 DD20 pKa = 3.32 RR21 pKa = 11.84 LCTRR25 pKa = 11.84 FLSALHH31 pKa = 5.48 NHH33 pKa = 6.98 DD34 pKa = 3.76 YY35 pKa = 10.97 YY36 pKa = 11.29 RR37 pKa = 11.84 FRR39 pKa = 11.84 FMDD42 pKa = 4.18 KK43 pKa = 10.31 III45 pKa = 4.03
Molecular weight: 5.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.196
IPC2_protein 9.414
IPC_protein 9.911
Toseland 9.736
ProMoST 9.663
Dawson 10.058
Bjellqvist 9.853
Wikipedia 10.306
Rodwell 10.16
Grimsley 10.175
Solomon 10.145
Lehninger 10.101
Nozaki 9.823
DTASelect 9.838
Thurlkill 9.867
EMBOSS 10.175
Sillero 9.999
Patrickios 7.571
IPC_peptide 10.131
IPC2_peptide 8.946
IPC2.peptide.svr19 8.241
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
71
0
71
13887
39
1339
195.6
22.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.992 ± 1.046
0.554 ± 0.103
6.337 ± 0.438
7.424 ± 0.613
4.133 ± 0.231
6.481 ± 0.632
1.548 ± 0.187
6.906 ± 0.183
8.872 ± 0.483
8.533 ± 0.28
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.412 ± 0.154
5.293 ± 0.295
2.376 ± 0.215
4.177 ± 0.197
4.407 ± 0.316
6.538 ± 0.449
6.128 ± 0.243
6.265 ± 0.28
1.023 ± 0.111
3.6 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here