Thiospirochaeta perfilievii

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Spirochaetaceae; Thiospirochaeta

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3264 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C1QHC0|A0A5C1QHC0_9SPIO RND family transporter OS=Thiospirochaeta perfilievii OX=252967 GN=EW093_15100 PE=4 SV=1
MM1 pKa = 7.59LLLTAGCLTTAPEE14 pKa = 4.43ANTKK18 pKa = 11.17DD19 pKa = 3.55MVEE22 pKa = 4.07AGAIEE27 pKa = 4.71DD28 pKa = 4.0GNVRR32 pKa = 11.84LHH34 pKa = 5.12YY35 pKa = 10.87QRR37 pKa = 11.84MDD39 pKa = 3.75DD40 pKa = 4.6NYY42 pKa = 10.16EE43 pKa = 3.67GWGLHH48 pKa = 5.02IWGAGYY54 pKa = 10.78AGDD57 pKa = 4.02TVDD60 pKa = 2.89WAAAVEE66 pKa = 4.23PTGFDD71 pKa = 3.82DD72 pKa = 6.63FGAYY76 pKa = 8.58WDD78 pKa = 3.68IPYY81 pKa = 10.52FGEE84 pKa = 5.21DD85 pKa = 3.15EE86 pKa = 4.72LNFIIHH92 pKa = 6.49NGDD95 pKa = 3.36EE96 pKa = 4.42KK97 pKa = 11.46DD98 pKa = 3.52PDD100 pKa = 3.59GDD102 pKa = 3.63RR103 pKa = 11.84TFEE106 pKa = 3.98GVEE109 pKa = 4.27TINEE113 pKa = 4.36FWALSGDD120 pKa = 3.9SVLYY124 pKa = 10.35TSLDD128 pKa = 3.72DD129 pKa = 3.85VQQ131 pKa = 4.94

Molecular weight: 14.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C1QCX4|A0A5C1QCX4_9SPIO Uncharacterized protein OS=Thiospirochaeta perfilievii OX=252967 GN=EW093_11265 PE=4 SV=1
MM1 pKa = 7.31ARR3 pKa = 11.84IAGIDD8 pKa = 3.88LPNKK12 pKa = 9.38HH13 pKa = 6.46LSIALTYY20 pKa = 9.86IYY22 pKa = 10.67GIGRR26 pKa = 11.84TTATQICDD34 pKa = 3.63KK35 pKa = 10.98AGLAHH40 pKa = 7.43DD41 pKa = 4.54VRR43 pKa = 11.84TADD46 pKa = 4.25LTQDD50 pKa = 3.67DD51 pKa = 4.56LGKK54 pKa = 10.54IKK56 pKa = 10.45DD57 pKa = 3.77IIEE60 pKa = 4.11NEE62 pKa = 4.11YY63 pKa = 9.54TVEE66 pKa = 3.74GRR68 pKa = 11.84LRR70 pKa = 11.84SEE72 pKa = 3.68VSLNIKK78 pKa = 10.03RR79 pKa = 11.84LMDD82 pKa = 3.8IGCYY86 pKa = 9.35RR87 pKa = 11.84GLRR90 pKa = 11.84HH91 pKa = 6.38RR92 pKa = 11.84RR93 pKa = 11.84KK94 pKa = 10.37LPVRR98 pKa = 11.84GQRR101 pKa = 11.84TKK103 pKa = 10.45TNARR107 pKa = 11.84TRR109 pKa = 11.84KK110 pKa = 9.52GKK112 pKa = 10.6AKK114 pKa = 9.56TVARR118 pKa = 11.84KK119 pKa = 9.8KK120 pKa = 10.44KK121 pKa = 10.14

Molecular weight: 13.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3264

0

3264

1103600

18

6887

338.1

38.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.752 ± 0.047

0.841 ± 0.015

6.083 ± 0.044

6.702 ± 0.057

4.941 ± 0.038

6.311 ± 0.057

1.459 ± 0.018

9.504 ± 0.049

7.98 ± 0.056

10.06 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.12 ± 0.024

6.144 ± 0.044

3.131 ± 0.023

2.409 ± 0.022

3.607 ± 0.034

7.635 ± 0.044

5.262 ± 0.067

6.052 ± 0.038

0.892 ± 0.015

4.116 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski