Tomato torrado virus (isolate Solanum lycopersicum/Spain/PRIToTV0301/-) (ToTV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Picornavirales; Secoviridae; Torradovirus; Tomato torrado virus

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A1XIQ1|POL2_TOTV RNA2 polyprotein OS=Tomato torrado virus (isolate Solanum lycopersicum/Spain/PRIToTV0301/-) OX=686948 PE=3 SV=1
MM1 pKa = 7.64SFISRR6 pKa = 11.84LNTSLEE12 pKa = 3.75EE13 pKa = 4.06EE14 pKa = 4.39AFHH17 pKa = 6.82KK18 pKa = 10.6QVADD22 pKa = 4.0SQWVCSVDD30 pKa = 3.25TGSGIINSDD39 pKa = 3.12PTLDD43 pKa = 4.8FKK45 pKa = 11.03ICPKK49 pKa = 9.72TGGAISVLSVSWQNNSPQLVPGHH72 pKa = 5.74YY73 pKa = 10.32LLRR76 pKa = 11.84SGTWPITGVKK86 pKa = 10.32LSGLLVHH93 pKa = 6.98RR94 pKa = 11.84SIRR97 pKa = 11.84LEE99 pKa = 3.97TTRR102 pKa = 11.84KK103 pKa = 9.7LLEE106 pKa = 3.94AQRR109 pKa = 11.84ISVSQQASSSSAAGAAGKK127 pKa = 9.63QPQVTLTQLQEE138 pKa = 4.24EE139 pKa = 4.37LDD141 pKa = 3.82EE142 pKa = 4.85AKK144 pKa = 9.99TRR146 pKa = 11.84LALKK150 pKa = 10.18EE151 pKa = 4.09KK152 pKa = 10.17EE153 pKa = 4.0LLEE156 pKa = 4.25ALSEE160 pKa = 4.07ISKK163 pKa = 10.49LRR165 pKa = 11.84LQLSNQLSNDD175 pKa = 3.46DD176 pKa = 4.28VFSGWTEE183 pKa = 3.98EE184 pKa = 4.54GPKK187 pKa = 10.35

Molecular weight: 20.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A1XIQ1|POL2_TOTV RNA2 polyprotein OS=Tomato torrado virus (isolate Solanum lycopersicum/Spain/PRIToTV0301/-) OX=686948 PE=3 SV=1
MM1 pKa = 7.12MMSSVAGQRR10 pKa = 11.84KK11 pKa = 9.1GPSDD15 pKa = 3.09KK16 pKa = 10.86SAAVSEE22 pKa = 3.98QLIAQITAAVEE33 pKa = 3.99AGNKK37 pKa = 9.32NLLRR41 pKa = 11.84KK42 pKa = 9.96LGMGSYY48 pKa = 10.47GILYY52 pKa = 8.87GAQEE56 pKa = 4.3KK57 pKa = 10.65KK58 pKa = 10.83AMEE61 pKa = 4.54LFDD64 pKa = 5.18PEE66 pKa = 4.44DD67 pKa = 3.44VHH69 pKa = 8.04NITSLWSSFKK79 pKa = 10.15KK80 pKa = 9.3TFTSSRR86 pKa = 11.84DD87 pKa = 3.2HH88 pKa = 6.8GNLFFHH94 pKa = 7.28LYY96 pKa = 10.04GVMFFMVPHH105 pKa = 4.83VHH107 pKa = 6.43GGEE110 pKa = 4.17GSVKK114 pKa = 10.24ISLCSSNDD122 pKa = 2.95PTNPVLQEE130 pKa = 3.68KK131 pKa = 10.15VLYY134 pKa = 10.2FSGGAQAVLMSPTITLPFVKK154 pKa = 9.56RR155 pKa = 11.84GPMFYY160 pKa = 10.23YY161 pKa = 10.55TMEE164 pKa = 4.43CLGTRR169 pKa = 11.84AQIPCSVVAIWKK181 pKa = 9.13QKK183 pKa = 9.82IDD185 pKa = 3.53IRR187 pKa = 11.84SAIYY191 pKa = 10.25SKK193 pKa = 10.89QEE195 pKa = 3.73TMSWAIEE202 pKa = 3.99ALHH205 pKa = 6.55RR206 pKa = 11.84PQFFQDD212 pKa = 3.13RR213 pKa = 11.84QEE215 pKa = 3.88AAQYY219 pKa = 10.21ISSVYY224 pKa = 11.1SNATSSATDD233 pKa = 3.21SVLPFVGAQLGDD245 pKa = 3.32TKK247 pKa = 10.98MNVPSEE253 pKa = 3.75ARR255 pKa = 11.84MIRR258 pKa = 11.84SSSLRR263 pKa = 11.84VPMLKK268 pKa = 9.89VQSKK272 pKa = 9.38RR273 pKa = 11.84FSSMEE278 pKa = 3.73IPSTSTAHH286 pKa = 7.04LLGTTRR292 pKa = 11.84DD293 pKa = 3.36EE294 pKa = 4.25TVIQEE299 pKa = 3.82EE300 pKa = 4.16SRR302 pKa = 11.84YY303 pKa = 9.96EE304 pKa = 3.89EE305 pKa = 4.48EE306 pKa = 5.41GDD308 pKa = 3.94DD309 pKa = 4.4GVLFPVKK316 pKa = 10.02KK317 pKa = 10.48AQGLNYY323 pKa = 10.27SHH325 pKa = 6.83VWDD328 pKa = 3.8NLGIEE333 pKa = 4.34SFVDD337 pKa = 3.65VEE339 pKa = 5.49LPEE342 pKa = 4.37NWDD345 pKa = 3.64EE346 pKa = 4.4LSVRR350 pKa = 11.84QQVAAAMIAFANKK363 pKa = 9.43GVCLVPKK370 pKa = 10.28HH371 pKa = 6.09IINRR375 pKa = 11.84DD376 pKa = 2.71KK377 pKa = 11.54HH378 pKa = 6.46NIHH381 pKa = 6.85LEE383 pKa = 4.04NITEE387 pKa = 4.06HH388 pKa = 6.6NYY390 pKa = 10.54LVILEE395 pKa = 4.52RR396 pKa = 11.84YY397 pKa = 9.15GIVNAGSLARR407 pKa = 11.84TEE409 pKa = 3.44NWYY412 pKa = 10.65NLTLAQRR419 pKa = 11.84VEE421 pKa = 3.86EE422 pKa = 5.0LIYY425 pKa = 10.83QRR427 pKa = 11.84DD428 pKa = 3.32DD429 pKa = 3.48AYY431 pKa = 11.5FMFGDD436 pKa = 3.79NTNPYY441 pKa = 9.94PPFDD445 pKa = 4.79CYY447 pKa = 11.31DD448 pKa = 3.51GLTLKK453 pKa = 10.26VRR455 pKa = 11.84SEE457 pKa = 4.22LEE459 pKa = 3.9RR460 pKa = 11.84VAKK463 pKa = 9.72EE464 pKa = 3.49QARR467 pKa = 11.84QRR469 pKa = 11.84FYY471 pKa = 11.54KK472 pKa = 10.02EE473 pKa = 3.14AARR476 pKa = 11.84AQVKK480 pKa = 10.57NKK482 pKa = 9.22VAQTSVEE489 pKa = 4.49EE490 pKa = 4.17IPSTSFATKK499 pKa = 9.94VAMEE503 pKa = 4.4SGSVDD508 pKa = 3.11SMKK511 pKa = 10.38IAIQAEE517 pKa = 4.16AANEE521 pKa = 3.76AVRR524 pKa = 11.84PNEE527 pKa = 3.56VMFEE531 pKa = 4.38FGQEE535 pKa = 4.05MNNEE539 pKa = 4.01GATEE543 pKa = 4.5LEE545 pKa = 4.48LQQPACVASNSFFNVGVFEE564 pKa = 4.31FAWKK568 pKa = 10.15KK569 pKa = 9.54SSSVAAEE576 pKa = 4.18VLSLALPAALFGKK589 pKa = 9.62SKK591 pKa = 9.86EE592 pKa = 4.05MSMGSQMLRR601 pKa = 11.84YY602 pKa = 9.29YY603 pKa = 10.75DD604 pKa = 3.95AALIMYY610 pKa = 9.11KK611 pKa = 10.38VILYY615 pKa = 9.67ISGMGAISGQLALVWDD631 pKa = 4.2EE632 pKa = 4.6CNVLNRR638 pKa = 11.84KK639 pKa = 9.54KK640 pKa = 10.71EE641 pKa = 4.27FINIASLYY649 pKa = 8.92ASKK652 pKa = 10.63HH653 pKa = 5.25RR654 pKa = 11.84LVSASEE660 pKa = 3.86QSSGEE665 pKa = 3.98FCFTPTGIGKK675 pKa = 8.15FVPLDD680 pKa = 3.94PASGAYY686 pKa = 9.86DD687 pKa = 3.65LGSIRR692 pKa = 11.84VFVTHH697 pKa = 7.12PLASATEE704 pKa = 4.29LEE706 pKa = 4.88SIPCHH711 pKa = 5.76IHH713 pKa = 5.81LQCKK717 pKa = 9.26VLSTNIMQPPRR728 pKa = 11.84LRR730 pKa = 11.84AQAQFGMKK738 pKa = 9.24PDD740 pKa = 3.44QTHH743 pKa = 6.61FPRR746 pKa = 11.84FPTNQVLLHH755 pKa = 5.74YY756 pKa = 9.13NWGVAASMGTTLVSIFSPSGIYY778 pKa = 10.36EE779 pKa = 4.06SDD781 pKa = 3.34GTLQPSLLGNIARR794 pKa = 11.84NCKK797 pKa = 7.76WWTGTCVFEE806 pKa = 4.01ICIEE810 pKa = 3.97KK811 pKa = 8.88TQFHH815 pKa = 6.65SGSLAIGLGTLNTSMSTPHH834 pKa = 7.59DD835 pKa = 4.16ILNMPHH841 pKa = 6.6VICNLEE847 pKa = 4.02MGRR850 pKa = 11.84KK851 pKa = 9.21FYY853 pKa = 10.6FRR855 pKa = 11.84CTITNWNGKK864 pKa = 8.59NLLTTGRR871 pKa = 11.84KK872 pKa = 9.24SSLPRR877 pKa = 11.84PKK879 pKa = 10.42HH880 pKa = 4.95MSHH883 pKa = 6.04MRR885 pKa = 11.84LFATVLKK892 pKa = 9.58PLVSTSIHH900 pKa = 7.0LDD902 pKa = 3.5TVGVTVQLKK911 pKa = 10.41CIEE914 pKa = 4.29DD915 pKa = 3.82LVLGGTVSVKK925 pKa = 10.22PIYY928 pKa = 10.11GHH930 pKa = 4.34WTKK933 pKa = 11.02GKK935 pKa = 9.77NAVDD939 pKa = 3.9FLFSEE944 pKa = 4.46MDD946 pKa = 3.71LSQRR950 pKa = 11.84KK951 pKa = 8.97EE952 pKa = 3.59IEE954 pKa = 3.91KK955 pKa = 10.27LRR957 pKa = 11.84KK958 pKa = 9.68EE959 pKa = 4.09NVEE962 pKa = 4.08TFDD965 pKa = 4.49EE966 pKa = 4.6KK967 pKa = 11.2GKK969 pKa = 10.12KK970 pKa = 8.62QPQVQVPLRR979 pKa = 11.84DD980 pKa = 3.47KK981 pKa = 10.73FSYY984 pKa = 10.59GAVQYY989 pKa = 10.81FVMNWKK995 pKa = 10.4DD996 pKa = 3.61EE997 pKa = 4.0EE998 pKa = 4.25RR999 pKa = 11.84LLVLPCAPWSVRR1011 pKa = 11.84FPQGALVQEE1020 pKa = 5.5AITCPFIDD1028 pKa = 3.66WCSSFCYY1035 pKa = 9.79WSGSLEE1041 pKa = 3.84YY1042 pKa = 9.98TIIVHH1047 pKa = 7.06RR1048 pKa = 11.84VQTSNNIGGVLNITLDD1064 pKa = 3.38SSGYY1068 pKa = 9.15PFPLGISKK1076 pKa = 8.4GTYY1079 pKa = 8.45VVSAGGGAKK1088 pKa = 8.96WAFTYY1093 pKa = 10.65GMSDD1097 pKa = 3.29NIFSFVVHH1105 pKa = 7.33DD1106 pKa = 4.82DD1107 pKa = 3.32EE1108 pKa = 5.35FFPRR1112 pKa = 11.84RR1113 pKa = 11.84HH1114 pKa = 4.97TKK1116 pKa = 10.04ARR1118 pKa = 11.84AIDD1121 pKa = 4.01PNASRR1126 pKa = 11.84IMTLQDD1132 pKa = 3.0RR1133 pKa = 11.84LGNLIINLPAKK1144 pKa = 10.36DD1145 pKa = 4.06VISSLEE1151 pKa = 3.85ILVKK1155 pKa = 10.41PGPDD1159 pKa = 3.26FKK1161 pKa = 11.26LQLAQAPSANHH1172 pKa = 6.07EE1173 pKa = 4.17KK1174 pKa = 10.66HH1175 pKa = 6.42LGDD1178 pKa = 4.0MQTHH1182 pKa = 6.59TYY1184 pKa = 10.56LYY1186 pKa = 9.85TPDD1189 pKa = 3.83FSEE1192 pKa = 4.91LRR1194 pKa = 11.84SFEE1197 pKa = 4.05NN1198 pKa = 3.5

Molecular weight: 133.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3543

187

2158

1181.0

131.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.79 ± 0.404

2.06 ± 0.322

4.968 ± 0.677

5.391 ± 0.627

5.222 ± 0.63

6.068 ± 0.182

2.427 ± 0.159

5.476 ± 0.123

5.504 ± 0.073

9.173 ± 0.782

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.738 ± 0.443

4.036 ± 0.267

4.516 ± 0.171

4.29 ± 0.533

4.347 ± 0.054

8.326 ± 1.1

5.504 ± 0.169

7.423 ± 0.355

1.411 ± 0.16

3.302 ± 0.552
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski