Celeribacter phage P12053L
Average proteome isoelectric point is 5.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I6S6K3|I6S6K3_9CAUD Putative phage tail fiber protein OS=Celeribacter phage P12053L OX=1197951 GN=P12053L_55 PE=4 SV=1
MM1 pKa = 7.59 AKK3 pKa = 10.07 KK4 pKa = 8.81 PTVTTLQSGFNSTEE18 pKa = 3.77 VLNSNFEE25 pKa = 4.19 KK26 pKa = 10.87 LRR28 pKa = 11.84 DD29 pKa = 3.79 AFDD32 pKa = 3.51 NTLSLDD38 pKa = 3.47 GSTPNAMEE46 pKa = 5.54 ADD48 pKa = 3.65 LDD50 pKa = 4.45 LNGNNLIGAVGLLVNGTDD68 pKa = 3.62 YY69 pKa = 11.12 LAEE72 pKa = 4.04 VEE74 pKa = 4.24 AAKK77 pKa = 10.41 AAALVAQAAAEE88 pKa = 4.08 TAEE91 pKa = 4.5 TNAEE95 pKa = 4.1 TAEE98 pKa = 4.44 TNAEE102 pKa = 4.16 TAEE105 pKa = 4.04 TAAASSASAASTSATNAATSATAASLSEE133 pKa = 3.83 TAAASSEE140 pKa = 4.29 ANTSASEE147 pKa = 4.06 TAAGLSEE154 pKa = 4.03 TAAATSEE161 pKa = 4.02 TAAALSEE168 pKa = 4.36 ANASASATAAATSATNASTSEE189 pKa = 4.07 TNAAASATEE198 pKa = 3.99 AATSEE203 pKa = 4.32 ANAATSATNASTSEE217 pKa = 4.12 TNAATSATAALASQTAAATSEE238 pKa = 4.72 SNASTSAANAATSEE252 pKa = 4.61 TNASTSASTASTKK265 pKa = 9.68 ATEE268 pKa = 4.37 AATSATNASTSEE280 pKa = 4.12 TNAATSATSAATSATSAQASKK301 pKa = 10.94 DD302 pKa = 3.5 AALAALDD309 pKa = 4.23 SFDD312 pKa = 5.84 DD313 pKa = 4.23 RR314 pKa = 11.84 YY315 pKa = 10.94 LGQKK319 pKa = 8.42 ATDD322 pKa = 3.64 PTLDD326 pKa = 3.42 NDD328 pKa = 3.56 GDD330 pKa = 4.24 ALVTGALYY338 pKa = 10.63 FNTADD343 pKa = 3.92 DD344 pKa = 3.68 VMKK347 pKa = 10.64 VYY349 pKa = 10.56 EE350 pKa = 4.21 GSVWVAAYY358 pKa = 10.26 ASLSGALLSANSLSDD373 pKa = 3.66 VLNAAAARR381 pKa = 11.84 TNLGLGTAATTASTDD396 pKa = 3.38 YY397 pKa = 9.88 ATAAQGSLADD407 pKa = 4.0 SALQSGDD414 pKa = 3.82 NISALTNDD422 pKa = 3.7 AGYY425 pKa = 6.08 TTNVGDD431 pKa = 3.89 ITNVSAGTGLSGGGASGTVTISHH454 pKa = 7.61 ADD456 pKa = 3.35 TSSQGSVNNSGATVIQDD473 pKa = 3.54 VTLDD477 pKa = 3.63 TYY479 pKa = 11.63 GHH481 pKa = 5.06 VTGLASKK488 pKa = 8.75 TLTAADD494 pKa = 3.44 VGAITGNQTITLSGDD509 pKa = 3.19 VSGSGTTSIVVTVADD524 pKa = 4.61 DD525 pKa = 3.6 SHH527 pKa = 7.82 NHH529 pKa = 5.91 VISNVDD535 pKa = 3.46 GLQTALDD542 pKa = 3.83 AKK544 pKa = 10.07 LASSSYY550 pKa = 8.92 TASDD554 pKa = 3.35 VLTKK558 pKa = 10.85 VKK560 pKa = 9.75 TVDD563 pKa = 3.49 GSGSGLDD570 pKa = 3.96 ADD572 pKa = 5.38 LLDD575 pKa = 4.65 GQHH578 pKa = 5.85 GSYY581 pKa = 10.51 YY582 pKa = 10.04 YY583 pKa = 10.89 SPSNPPAASAPSSAQVGSATAGLSVGAVGTYY614 pKa = 10.32 AYY616 pKa = 10.46 LYY618 pKa = 8.5 YY619 pKa = 10.53 YY620 pKa = 9.91 PRR622 pKa = 11.84 TTTSPGTTRR631 pKa = 11.84 AGSDD635 pKa = 2.95 LRR637 pKa = 11.84 YY638 pKa = 9.69 ANGYY642 pKa = 8.54 AAHH645 pKa = 6.57 TGYY648 pKa = 9.18 TGGGTPSGTWRR659 pKa = 11.84 CMGYY663 pKa = 9.72 IFDD666 pKa = 4.01 QAGGTGTDD674 pKa = 3.37 TTTVWLRR681 pKa = 11.84 ISS683 pKa = 3.26
Molecular weight: 66.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.872
IPC_protein 3.897
Toseland 3.668
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.719
Grimsley 3.579
Solomon 3.872
Lehninger 3.834
Nozaki 3.986
DTASelect 4.228
Thurlkill 3.719
EMBOSS 3.808
Sillero 4.012
Patrickios 1.392
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.889
Protein with the highest isoelectric point:
>tr|I6RT32|I6RT32_9CAUD Uncharacterized protein OS=Celeribacter phage P12053L OX=1197951 GN=P12053L_48 PE=4 SV=1
MM1 pKa = 7.87 AIFTAIGAALTAATGAAAFTAGVATVGAVVGVGGTIASGKK41 pKa = 8.38 AARR44 pKa = 11.84 RR45 pKa = 11.84 AASAQQKK52 pKa = 9.68 SQDD55 pKa = 3.76 LQAKK59 pKa = 6.99 RR60 pKa = 11.84 QRR62 pKa = 11.84 RR63 pKa = 11.84 AAIRR67 pKa = 11.84 SNILASARR75 pKa = 11.84 ARR77 pKa = 11.84 ASAQSAGTAQSSGLSGAIGAGRR99 pKa = 11.84 SQLGAEE105 pKa = 4.56 LGFGTQMSGLSANISKK121 pKa = 10.64 FNMQAQTYY129 pKa = 10.35 GDD131 pKa = 3.44 IAKK134 pKa = 10.27 LGFSAAGNAPALGGYY149 pKa = 10.03 AEE151 pKa = 4.58 EE152 pKa = 4.65 FGLFNKK158 pKa = 10.19
Molecular weight: 15.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.238
IPC2_protein 10.072
IPC_protein 11.199
Toseland 11.359
ProMoST 11.652
Dawson 11.389
Bjellqvist 11.272
Wikipedia 11.754
Rodwell 11.447
Grimsley 11.433
Solomon 11.754
Lehninger 11.681
Nozaki 11.345
DTASelect 11.272
Thurlkill 11.359
EMBOSS 11.828
Sillero 11.359
Patrickios 11.199
IPC_peptide 11.754
IPC2_peptide 10.628
IPC2.peptide.svr19 8.87
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
56
0
56
12540
46
1487
223.9
24.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.123 ± 0.833
0.949 ± 0.192
6.818 ± 0.212
7.073 ± 0.28
3.596 ± 0.224
7.121 ± 0.301
1.595 ± 0.167
4.777 ± 0.308
6.643 ± 0.442
8.094 ± 0.182
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.576 ± 0.167
4.609 ± 0.195
3.246 ± 0.179
3.517 ± 0.204
4.617 ± 0.321
6.89 ± 0.499
6.834 ± 0.516
6.722 ± 0.278
1.38 ± 0.18
3.82 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here