Microlunatus sagamiharensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; Microlunatus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3981 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H2N7P1|A0A1H2N7P1_9ACTN Uncharacterized protein OS=Microlunatus sagamiharensis OX=546874 GN=SAMN04488544_3436 PE=4 SV=1
MM1 pKa = 7.29TTTQDD6 pKa = 3.47YY7 pKa = 10.23VVAGMTCEE15 pKa = 4.14HH16 pKa = 6.72CTQAVSEE23 pKa = 4.5EE24 pKa = 4.27VLAVPGVRR32 pKa = 11.84EE33 pKa = 4.26VTVHH37 pKa = 6.87LDD39 pKa = 3.15GGTMTVTSEE48 pKa = 4.57DD49 pKa = 4.6PIDD52 pKa = 4.37FDD54 pKa = 4.33ALSAAVDD61 pKa = 3.54EE62 pKa = 4.99AGYY65 pKa = 8.64TLGSRR70 pKa = 4.08

Molecular weight: 7.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H2N719|A0A1H2N719_9ACTN Putative spermidine/putrescine transport system ATP-binding protein OS=Microlunatus sagamiharensis OX=546874 GN=SAMN04488544_3455 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SRR15 pKa = 11.84THH17 pKa = 6.18GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.39GRR40 pKa = 11.84AKK42 pKa = 10.68LSAA45 pKa = 3.92

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3981

0

3981

1314726

31

7955

330.3

35.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.573 ± 0.061

0.649 ± 0.012

6.259 ± 0.037

5.303 ± 0.047

2.712 ± 0.025

9.515 ± 0.038

1.971 ± 0.021

2.719 ± 0.03

1.722 ± 0.032

10.54 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.542 ± 0.016

1.659 ± 0.022

5.877 ± 0.029

2.765 ± 0.022

7.791 ± 0.049

5.501 ± 0.039

6.349 ± 0.083

10.079 ± 0.042

1.502 ± 0.019

1.974 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski