Streptococcus phage Javan345
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A8B3|A0A4D6A8B3_9CAUD Cell wall-associated murein hydrolase OS=Streptococcus phage Javan345 OX=2548115 GN=Javan345_0044 PE=4 SV=1
MM1 pKa = 6.98 NQNNDD6 pKa = 3.15 FAEE9 pKa = 4.14 VLLEE13 pKa = 4.06 YY14 pKa = 10.53 IKK16 pKa = 10.76 GIQDD20 pKa = 4.6 KK21 pKa = 10.51 IPSKK25 pKa = 10.52 HH26 pKa = 6.11 GYY28 pKa = 8.93 LAEE31 pKa = 4.35 KK32 pKa = 9.79 EE33 pKa = 4.3 GLVVFPLAGGEE44 pKa = 4.31 VVDD47 pKa = 4.74 EE48 pKa = 4.72 DD49 pKa = 3.69 MAGIQTVSLPFEE61 pKa = 3.99 IAIKK65 pKa = 10.84 SRR67 pKa = 11.84 DD68 pKa = 3.43 QEE70 pKa = 4.56 LNNNTLWQINAALSKK85 pKa = 10.22 MGLEE89 pKa = 4.39 LPSKK93 pKa = 9.38 NGSYY97 pKa = 10.54 EE98 pKa = 3.95 FLGLKK103 pKa = 9.26 VDD105 pKa = 4.17 KK106 pKa = 10.45 PYY108 pKa = 11.37 LNDD111 pKa = 3.67 LDD113 pKa = 4.0 EE114 pKa = 4.51 QGFYY118 pKa = 10.26 IYY120 pKa = 10.75 LLDD123 pKa = 3.55 VTASLEE129 pKa = 4.04 IEE131 pKa = 4.16 RR132 pKa = 11.84 NEE134 pKa = 3.88
Molecular weight: 15.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.407
IPC2_protein 4.342
IPC_protein 4.24
Toseland 4.075
ProMoST 4.304
Dawson 4.19
Bjellqvist 4.393
Wikipedia 4.062
Rodwell 4.075
Grimsley 3.986
Solomon 4.19
Lehninger 4.139
Nozaki 4.304
DTASelect 4.444
Thurlkill 4.088
EMBOSS 4.075
Sillero 4.355
Patrickios 3.719
IPC_peptide 4.19
IPC2_peptide 4.342
IPC2.peptide.svr19 4.283
Protein with the highest isoelectric point:
>tr|A0A4D6ACF3|A0A4D6ACF3_9CAUD Uncharacterized protein OS=Streptococcus phage Javan345 OX=2548115 GN=Javan345_0042 PE=4 SV=1
MM1 pKa = 7.42 GGVNVKK7 pKa = 9.78 IDD9 pKa = 3.38 LSGIEE14 pKa = 4.44 KK15 pKa = 10.3 KK16 pKa = 10.84 VSPEE20 pKa = 3.5 NLAKK24 pKa = 10.67 GKK26 pKa = 9.96 LAIANQMLMDD36 pKa = 4.12 MEE38 pKa = 4.55 RR39 pKa = 11.84 FVPKK43 pKa = 10.34 RR44 pKa = 11.84 KK45 pKa = 10.22 GEE47 pKa = 4.0 LRR49 pKa = 11.84 SSGHH53 pKa = 5.14 VRR55 pKa = 11.84 QDD57 pKa = 3.5 SIVYY61 pKa = 6.47 ATPYY65 pKa = 10.94 ARR67 pKa = 11.84 LLYY70 pKa = 10.06 YY71 pKa = 10.27 GKK73 pKa = 9.95 KK74 pKa = 9.48 RR75 pKa = 11.84 KK76 pKa = 9.93 GFFSEE81 pKa = 3.79 KK82 pKa = 8.59 QRR84 pKa = 11.84 RR85 pKa = 11.84 FFFANKK91 pKa = 9.4 EE92 pKa = 4.04 KK93 pKa = 10.74 LLSQRR98 pKa = 11.84 PTPGTGPRR106 pKa = 11.84 WDD108 pKa = 3.54 KK109 pKa = 10.92 KK110 pKa = 10.61 AAALHH115 pKa = 5.36 SKK117 pKa = 9.74 KK118 pKa = 9.92 WGDD121 pKa = 3.49 VGLKK125 pKa = 10.86 AMGLKK130 pKa = 10.15
Molecular weight: 14.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.208
IPC2_protein 9.75
IPC_protein 9.897
Toseland 10.76
ProMoST 10.292
Dawson 10.833
Bjellqvist 10.423
Wikipedia 10.95
Rodwell 11.418
Grimsley 10.862
Solomon 10.877
Lehninger 10.862
Nozaki 10.716
DTASelect 10.423
Thurlkill 10.73
EMBOSS 11.125
Sillero 10.745
Patrickios 11.14
IPC_peptide 10.891
IPC2_peptide 8.858
IPC2.peptide.svr19 8.735
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
44
0
44
10764
43
1214
244.6
27.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.395 ± 0.996
0.641 ± 0.116
6.262 ± 0.34
7.646 ± 0.586
4.218 ± 0.237
6.475 ± 0.44
1.329 ± 0.207
6.531 ± 0.318
8.417 ± 0.604
7.925 ± 0.278
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.369 ± 0.251
5.323 ± 0.411
2.573 ± 0.171
4.469 ± 0.244
3.995 ± 0.336
6.819 ± 0.514
5.946 ± 0.424
6.615 ± 0.331
1.301 ± 0.209
3.753 ± 0.395
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here